Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients’ Stomachs
Shadiyeh Khani, Amin Talebi Bezmin Abadi, Ashraf Mohabati Mobarez Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranCorrespondence: Amin Talebi Bezmin AbadiDepartment of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14115...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-11-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/clarithromycin-susceptible-but-virulent-helicobacter-pylori-strains-in-peer-reviewed-article-IDR |
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| author | Khani S Talebi Bezmin Abadi A Mohabati Mobarez A |
| author_facet | Khani S Talebi Bezmin Abadi A Mohabati Mobarez A |
| author_sort | Khani S |
| collection | DOAJ |
| description | Shadiyeh Khani, Amin Talebi Bezmin Abadi, Ashraf Mohabati Mobarez Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranCorrespondence: Amin Talebi Bezmin AbadiDepartment of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14115-111, IranTel +98 21 8288 4883Email Amin.talebi@modares.ac.irIntroduction: Helicobacter pylori was discovered first in the stomachs of patients with gastritis and ulcers by Marshall and Warren in 1982. This discovery majorly affected many research areas of gastroenterology. Since then, the main aim has been to eradicate this microaerophilic bacterium from the stomachs of infected subjects.Methods: We studied symptomatic cases by endoscopic surgery and examined the prevalence of cagA–vacA genotypes among the H. pylori isolates. H. pylori isolated from antral biopsies of patients with gastritis and duodenal ulcer were subjected to antimicrobial susceptibility testing and PCR genotyping by using routine bacterial cultures. Clarithromycin-susceptibility profiling was done by the E-test. DNA was extracted using standard manufacturer protocols with minor modifications and cagA and vacA genotyping was done PCR.Results: In our study, all strains identified as H. pylori in culture (61/81) were confirmed by PCR by amplifying a fragment of the glmM gene. Totally, 61 patients were confirmed to be positive for H. pylori and they were included in the genotyping and antibiotic-susceptibility testing. Thirteen H. pylori strains were determined to be resistant to clarithromycin.Discussion: Current accumulating data indicate that both clarithromycin-resistant and susceptible isolates of H. pylori need to be screened and tracked in populations.Keywords: antibiotic susceptibility, cagA, clarithromycin, E-test, Helicobacter pylori, genotypes, PCR, vacA |
| first_indexed | 2024-12-20T05:18:37Z |
| format | Article |
| id | doaj.art-0d16ccb50a7f424db2158080723646c1 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-20T05:18:37Z |
| publishDate | 2019-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-0d16ccb50a7f424db2158080723646c12022-12-21T19:52:04ZengDove Medical PressInfection and Drug Resistance1178-69732019-11-01Volume 123415342049451Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients’ StomachsKhani STalebi Bezmin Abadi AMohabati Mobarez AShadiyeh Khani, Amin Talebi Bezmin Abadi, Ashraf Mohabati Mobarez Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranCorrespondence: Amin Talebi Bezmin AbadiDepartment of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14115-111, IranTel +98 21 8288 4883Email Amin.talebi@modares.ac.irIntroduction: Helicobacter pylori was discovered first in the stomachs of patients with gastritis and ulcers by Marshall and Warren in 1982. This discovery majorly affected many research areas of gastroenterology. Since then, the main aim has been to eradicate this microaerophilic bacterium from the stomachs of infected subjects.Methods: We studied symptomatic cases by endoscopic surgery and examined the prevalence of cagA–vacA genotypes among the H. pylori isolates. H. pylori isolated from antral biopsies of patients with gastritis and duodenal ulcer were subjected to antimicrobial susceptibility testing and PCR genotyping by using routine bacterial cultures. Clarithromycin-susceptibility profiling was done by the E-test. DNA was extracted using standard manufacturer protocols with minor modifications and cagA and vacA genotyping was done PCR.Results: In our study, all strains identified as H. pylori in culture (61/81) were confirmed by PCR by amplifying a fragment of the glmM gene. Totally, 61 patients were confirmed to be positive for H. pylori and they were included in the genotyping and antibiotic-susceptibility testing. Thirteen H. pylori strains were determined to be resistant to clarithromycin.Discussion: Current accumulating data indicate that both clarithromycin-resistant and susceptible isolates of H. pylori need to be screened and tracked in populations.Keywords: antibiotic susceptibility, cagA, clarithromycin, E-test, Helicobacter pylori, genotypes, PCR, vacAhttps://www.dovepress.com/clarithromycin-susceptible-but-virulent-helicobacter-pylori-strains-in-peer-reviewed-article-IDRantibiotic susceptibilitycagaclarithromycine-testhelicobacter pylorigenotypespcrvaca |
| spellingShingle | Khani S Talebi Bezmin Abadi A Mohabati Mobarez A Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients’ Stomachs Infection and Drug Resistance antibiotic susceptibility caga clarithromycin e-test helicobacter pylori genotypes pcr vaca |
| title | Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients’ Stomachs |
| title_full | Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients’ Stomachs |
| title_fullStr | Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients’ Stomachs |
| title_full_unstemmed | Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients’ Stomachs |
| title_short | Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients’ Stomachs |
| title_sort | clarithromycin susceptible but virulent helicobacter pylori strains infecting iranian patients stomachs |
| topic | antibiotic susceptibility caga clarithromycin e-test helicobacter pylori genotypes pcr vaca |
| url | https://www.dovepress.com/clarithromycin-susceptible-but-virulent-helicobacter-pylori-strains-in-peer-reviewed-article-IDR |
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