Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain
Immune function plays a pivotal role in dictating the overall health and productivity of cattle. In a proficient immune system, the liver assumes an integral function in detoxification and metabolic processes and contributes substantially to overall production and immunity. In this study, we evaluat...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Animal Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fanim.2024.1349499/full |
| _version_ | 1797238935565369344 |
|---|---|
| author | Deborah Ologunagba Modoluwamu Idowu Godstime Taiwo Taylor Sidney Emily Treon Francisca Eichie Frederick Bebe Frederick Bebe Ibukun M. Ogunade |
| author_facet | Deborah Ologunagba Modoluwamu Idowu Godstime Taiwo Taylor Sidney Emily Treon Francisca Eichie Frederick Bebe Frederick Bebe Ibukun M. Ogunade |
| author_sort | Deborah Ologunagba |
| collection | DOAJ |
| description | Immune function plays a pivotal role in dictating the overall health and productivity of cattle. In a proficient immune system, the liver assumes an integral function in detoxification and metabolic processes and contributes substantially to overall production and immunity. In this study, we evaluated the hepatic mRNA expression of genes involved in innate and adaptive immunity in crossbred beef steers with positive or negative residual body weight gain (RADG). Positive-RADG beef steers (n = 8; RADG = 0.73 kg/d) and negative-RADG beef steers (n = 8; RADG = -0.69 kg/d) were identified from a group of 108 growing crossbred beef steers (average BW = 556 ± 38 kg) after a 56-d performance testing period. At the end of the 56-d period, liver tissue samples were collected from the beef steers for RNA extraction and cDNA synthesis. The mRNA expression of 84 genes involved in innate and adaptive immunity were analyzed using pathway-focused PCR-based arrays. The mRNA expression of genes with false discovery rate-adjusted P-values (FDR) ≤ 0.05 and absolute fold change (FC) ≥ 1.2 were determined to be differentially expressed. Out of the 84 genes analyzed, four genes (IL-2, MYD88, CD-80, NFkB-1) were differentially expressed and were all upregulated in positive compared with negative-RADG beef steers. IL-2 is a cytokine that plays a critical role in the immune response by activating and proliferating T-cells, which are important for fighting infections. MYD88 is an adaptor protein that is essential for signaling by toll-like receptors, which are involved in pathogen recognition. CD80 is a protein that is expressed on the surface of antigen-presenting cells and plays a critical role in the initiation of an immune response. The activation of NF-κB leads to the production of cytokines and chemokines that help to recruit immune cells to the site of infection. The upregulation of the aforementioned genes in positive-RADG beef steers suggests that they had a better ability than negative-RADG beef steers to quickly recognize pathogens and initiate appropriate responses to effectively fight off infections without causing inflammatory reactions, potentially contributing to their better feed efficiency. |
| first_indexed | 2024-04-24T17:43:33Z |
| format | Article |
| id | doaj.art-0d1b4a2a60fe4af5affd122ee300b35a |
| institution | Directory Open Access Journal |
| issn | 2673-6225 |
| language | English |
| last_indexed | 2024-04-24T17:43:33Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Animal Science |
| spelling | doaj.art-0d1b4a2a60fe4af5affd122ee300b35a2024-03-27T17:45:06ZengFrontiers Media S.A.Frontiers in Animal Science2673-62252024-03-01510.3389/fanim.2024.13494991349499Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gainDeborah Ologunagba0Modoluwamu Idowu1Godstime Taiwo2Taylor Sidney3Emily Treon4Francisca Eichie5Frederick Bebe6Frederick Bebe7Ibukun M. Ogunade8Division of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United StatesDivision of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United StatesDivision of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United StatesDivision of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United StatesDivision of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United StatesDivision of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United StatesDivision of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United StatesCollege of Agriculture, Health and Natural Resources, Kentucky State University, Frankfort, KY, United StatesDivision of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United StatesImmune function plays a pivotal role in dictating the overall health and productivity of cattle. In a proficient immune system, the liver assumes an integral function in detoxification and metabolic processes and contributes substantially to overall production and immunity. In this study, we evaluated the hepatic mRNA expression of genes involved in innate and adaptive immunity in crossbred beef steers with positive or negative residual body weight gain (RADG). Positive-RADG beef steers (n = 8; RADG = 0.73 kg/d) and negative-RADG beef steers (n = 8; RADG = -0.69 kg/d) were identified from a group of 108 growing crossbred beef steers (average BW = 556 ± 38 kg) after a 56-d performance testing period. At the end of the 56-d period, liver tissue samples were collected from the beef steers for RNA extraction and cDNA synthesis. The mRNA expression of 84 genes involved in innate and adaptive immunity were analyzed using pathway-focused PCR-based arrays. The mRNA expression of genes with false discovery rate-adjusted P-values (FDR) ≤ 0.05 and absolute fold change (FC) ≥ 1.2 were determined to be differentially expressed. Out of the 84 genes analyzed, four genes (IL-2, MYD88, CD-80, NFkB-1) were differentially expressed and were all upregulated in positive compared with negative-RADG beef steers. IL-2 is a cytokine that plays a critical role in the immune response by activating and proliferating T-cells, which are important for fighting infections. MYD88 is an adaptor protein that is essential for signaling by toll-like receptors, which are involved in pathogen recognition. CD80 is a protein that is expressed on the surface of antigen-presenting cells and plays a critical role in the initiation of an immune response. The activation of NF-κB leads to the production of cytokines and chemokines that help to recruit immune cells to the site of infection. The upregulation of the aforementioned genes in positive-RADG beef steers suggests that they had a better ability than negative-RADG beef steers to quickly recognize pathogens and initiate appropriate responses to effectively fight off infections without causing inflammatory reactions, potentially contributing to their better feed efficiency.https://www.frontiersin.org/articles/10.3389/fanim.2024.1349499/fullfeed efficiencyimmunitygene expressionliverbeef cattle |
| spellingShingle | Deborah Ologunagba Modoluwamu Idowu Godstime Taiwo Taylor Sidney Emily Treon Francisca Eichie Frederick Bebe Frederick Bebe Ibukun M. Ogunade Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain Frontiers in Animal Science feed efficiency immunity gene expression liver beef cattle |
| title | Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain |
| title_full | Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain |
| title_fullStr | Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain |
| title_full_unstemmed | Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain |
| title_short | Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain |
| title_sort | hepatic mrna expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain |
| topic | feed efficiency immunity gene expression liver beef cattle |
| url | https://www.frontiersin.org/articles/10.3389/fanim.2024.1349499/full |
| work_keys_str_mv | AT deborahologunagba hepaticmrnaexpressionofinnateandadaptiveimmunegenesinbeefsteerswithdivergentresidualbodyweightgain AT modoluwamuidowu hepaticmrnaexpressionofinnateandadaptiveimmunegenesinbeefsteerswithdivergentresidualbodyweightgain AT godstimetaiwo hepaticmrnaexpressionofinnateandadaptiveimmunegenesinbeefsteerswithdivergentresidualbodyweightgain AT taylorsidney hepaticmrnaexpressionofinnateandadaptiveimmunegenesinbeefsteerswithdivergentresidualbodyweightgain AT emilytreon hepaticmrnaexpressionofinnateandadaptiveimmunegenesinbeefsteerswithdivergentresidualbodyweightgain AT franciscaeichie hepaticmrnaexpressionofinnateandadaptiveimmunegenesinbeefsteerswithdivergentresidualbodyweightgain AT frederickbebe hepaticmrnaexpressionofinnateandadaptiveimmunegenesinbeefsteerswithdivergentresidualbodyweightgain AT frederickbebe hepaticmrnaexpressionofinnateandadaptiveimmunegenesinbeefsteerswithdivergentresidualbodyweightgain AT ibukunmogunade hepaticmrnaexpressionofinnateandadaptiveimmunegenesinbeefsteerswithdivergentresidualbodyweightgain |