Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip
Spheroid, a 3D aggregate of tumor cells in a spherical shape, has overcome the limitations of conventional 3D cell models to accurately mimic the in-vivo environment of a human body. The spheroids are cultured with other primary cells and embedded in collagen drops using hang drop plates and low-att...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/11/12/506 |
| _version_ | 1797410803487342592 |
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| author | Hyewon Roh Hwisoo Kim Je-Kyun Park |
| author_facet | Hyewon Roh Hwisoo Kim Je-Kyun Park |
| author_sort | Hyewon Roh |
| collection | DOAJ |
| description | Spheroid, a 3D aggregate of tumor cells in a spherical shape, has overcome the limitations of conventional 3D cell models to accurately mimic the in-vivo environment of a human body. The spheroids are cultured with other primary cells and embedded in collagen drops using hang drop plates and low-attachment well plates to construct a spheroid–hydrogel model that better mimics the cell–cell and cell–extracellular matrix (ECM) interactions. However, the conventional methods of culturing and embedding spheroids into ECM have several shortcomings. The procedure of transferring a single spheroid at a time by manual pipetting results in well-to-well variation and even loss or damage of the spheroid. Based on the previously introduced droplet contact-based spheroid transfer technique, we present a poly(dimethylsiloxane) and resin-based drop array chip and a pillar array chip with alignment stoppers, which enhances the alignment between the chips for uniform placement of spheroids. This method allows the facile and stable transfer of the spheroid array and even eliminates the need for a stereomicroscope while handling the cell models. The novel platform demonstrates a homogeneous and time-efficient construction and diverse analysis of an array of fibroblast-associated glioblastoma multiforme spheroids that are embedded in collagen. |
| first_indexed | 2024-03-09T04:35:31Z |
| format | Article |
| id | doaj.art-0d1ebf21952c4c65a78af10711f7e8ee |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T04:35:31Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-0d1ebf21952c4c65a78af10711f7e8ee2023-12-03T13:28:12ZengMDPI AGBiosensors2079-63742021-12-01111250610.3390/bios11120506Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array ChipHyewon Roh0Hwisoo Kim1Je-Kyun Park2Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, KoreaDepartment of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, KoreaDepartment of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, KoreaSpheroid, a 3D aggregate of tumor cells in a spherical shape, has overcome the limitations of conventional 3D cell models to accurately mimic the in-vivo environment of a human body. The spheroids are cultured with other primary cells and embedded in collagen drops using hang drop plates and low-attachment well plates to construct a spheroid–hydrogel model that better mimics the cell–cell and cell–extracellular matrix (ECM) interactions. However, the conventional methods of culturing and embedding spheroids into ECM have several shortcomings. The procedure of transferring a single spheroid at a time by manual pipetting results in well-to-well variation and even loss or damage of the spheroid. Based on the previously introduced droplet contact-based spheroid transfer technique, we present a poly(dimethylsiloxane) and resin-based drop array chip and a pillar array chip with alignment stoppers, which enhances the alignment between the chips for uniform placement of spheroids. This method allows the facile and stable transfer of the spheroid array and even eliminates the need for a stereomicroscope while handling the cell models. The novel platform demonstrates a homogeneous and time-efficient construction and diverse analysis of an array of fibroblast-associated glioblastoma multiforme spheroids that are embedded in collagen.https://www.mdpi.com/2079-6374/11/12/5063D cell culturedroplet contact-based spheroid transferdrug assay modelfibroblastglioblastoma multiformespheroid invasion assay |
| spellingShingle | Hyewon Roh Hwisoo Kim Je-Kyun Park Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip Biosensors 3D cell culture droplet contact-based spheroid transfer drug assay model fibroblast glioblastoma multiforme spheroid invasion assay |
| title | Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip |
| title_full | Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip |
| title_fullStr | Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip |
| title_full_unstemmed | Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip |
| title_short | Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip |
| title_sort | construction of a fibroblast associated tumor spheroid model based on a collagen drop array chip |
| topic | 3D cell culture droplet contact-based spheroid transfer drug assay model fibroblast glioblastoma multiforme spheroid invasion assay |
| url | https://www.mdpi.com/2079-6374/11/12/506 |
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