HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas
Abstract Chordomas are rare tumours of the bone arising along the spine from clivus to sacrum. We compared three chordoma cell lines of the clivus region including the newly established clivus chordoma cell line, U-CH14, with nine chordoma cell lines originating from sacral primaries by morphology,...
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Nature Portfolio
2017-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-02174-5 |
| _version_ | 1818839764758953984 |
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| author | Daniela Jäger Thomas F. E. Barth Silke Brüderlein Angelika Scheuerle Beate Rinner Adrian von Witzleben André Lechel Patrick Meyer Regine Mayer-Steinacker Alexandra von Baer Markus Schultheiss Christian R. Wirtz Peter Möller Kevin Mellert |
| author_facet | Daniela Jäger Thomas F. E. Barth Silke Brüderlein Angelika Scheuerle Beate Rinner Adrian von Witzleben André Lechel Patrick Meyer Regine Mayer-Steinacker Alexandra von Baer Markus Schultheiss Christian R. Wirtz Peter Möller Kevin Mellert |
| author_sort | Daniela Jäger |
| collection | DOAJ |
| description | Abstract Chordomas are rare tumours of the bone arising along the spine from clivus to sacrum. We compared three chordoma cell lines of the clivus region including the newly established clivus chordoma cell line, U-CH14, with nine chordoma cell lines originating from sacral primaries by morphology, on genomic and expression levels and with patient samples from our chordoma tissue bank. Clinically, chordomas of the clivus were generally smaller in size at presentation and patients with sacral chordomas had more metastases and more often recurrent disease. All chordoma cell lines had a typical physaliphorous morphology and expressed brachyury, S100-protein and cytokeratin. By expression analyses we detected differentially expressed genes in the clivus derived cell lines as compared to the sacral cell lines. Among these were HOXA7, HOXA9, and HOXA10 known to be important for the development of the anterior-posterior body axis. These results were confirmed by qPCR. Immunohistologically, clivus chordomas had no or very low levels of HOXA10 protein while sacral chordomas showed a strong nuclear positivity in all samples analysed. This differential expression of HOX genes in chordomas of the clivus and sacrum suggests an oncofetal mechanism in gene regulation linked to the anatomic site. |
| first_indexed | 2024-12-19T03:59:29Z |
| format | Article |
| id | doaj.art-0d1eed0325ea49689979e4d5afd31ace |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T03:59:29Z |
| publishDate | 2017-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0d1eed0325ea49689979e4d5afd31ace2022-12-21T20:36:43ZengNature PortfolioScientific Reports2045-23222017-05-017111210.1038/s41598-017-02174-5HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomasDaniela Jäger0Thomas F. E. Barth1Silke Brüderlein2Angelika Scheuerle3Beate Rinner4Adrian von Witzleben5André Lechel6Patrick Meyer7Regine Mayer-Steinacker8Alexandra von Baer9Markus Schultheiss10Christian R. Wirtz11Peter Möller12Kevin Mellert13Institute of Pathology, University of UlmInstitute of Pathology, University of UlmInstitute of Pathology, University of UlmInstitute of Pathology, University of UlmDivision of Biomedical Research, Medical University of GrazInstitute of Pathology, University of UlmDepartment of Internal Medicine I, University of UlmDepartment of Dermatology, University of UlmDepartment of Internal Medicine III, University of UlmDepartment of Trauma Surgery, University of UlmDepartment of Trauma Surgery, University of UlmDepartment of Neurosurgery, University of UlmInstitute of Pathology, University of UlmInstitute of Pathology, University of UlmAbstract Chordomas are rare tumours of the bone arising along the spine from clivus to sacrum. We compared three chordoma cell lines of the clivus region including the newly established clivus chordoma cell line, U-CH14, with nine chordoma cell lines originating from sacral primaries by morphology, on genomic and expression levels and with patient samples from our chordoma tissue bank. Clinically, chordomas of the clivus were generally smaller in size at presentation and patients with sacral chordomas had more metastases and more often recurrent disease. All chordoma cell lines had a typical physaliphorous morphology and expressed brachyury, S100-protein and cytokeratin. By expression analyses we detected differentially expressed genes in the clivus derived cell lines as compared to the sacral cell lines. Among these were HOXA7, HOXA9, and HOXA10 known to be important for the development of the anterior-posterior body axis. These results were confirmed by qPCR. Immunohistologically, clivus chordomas had no or very low levels of HOXA10 protein while sacral chordomas showed a strong nuclear positivity in all samples analysed. This differential expression of HOX genes in chordomas of the clivus and sacrum suggests an oncofetal mechanism in gene regulation linked to the anatomic site.https://doi.org/10.1038/s41598-017-02174-5 |
| spellingShingle | Daniela Jäger Thomas F. E. Barth Silke Brüderlein Angelika Scheuerle Beate Rinner Adrian von Witzleben André Lechel Patrick Meyer Regine Mayer-Steinacker Alexandra von Baer Markus Schultheiss Christian R. Wirtz Peter Möller Kevin Mellert HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas Scientific Reports |
| title | HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas |
| title_full | HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas |
| title_fullStr | HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas |
| title_full_unstemmed | HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas |
| title_short | HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas |
| title_sort | hoxa7 hoxa9 and hoxa10 are differentially expressed in clival and sacral chordomas |
| url | https://doi.org/10.1038/s41598-017-02174-5 |
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