Beneficial Proapoptotic Effect of Heterobasidion Annosum Extract in Colorectal Cancer Xenograft Mouse Model
Fungal extracts possess potential anticancer activity against many malignant neoplastic diseases. In this research, we focused on the evaluation of <i>Heterobasidion annosum</i> (HA) extract in colorectal cancer in an in vivo model. The mice with implanted DLD-1 human cancer cells were g...
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MDPI AG
2023-01-01
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| author | Anna Sadowska Diana Sawicka Katarzyna Godlewska Katarzyna Guzińska-Ustymowicz Ewa Zapora Emilia Sokołowska Halina Car |
| author_facet | Anna Sadowska Diana Sawicka Katarzyna Godlewska Katarzyna Guzińska-Ustymowicz Ewa Zapora Emilia Sokołowska Halina Car |
| author_sort | Anna Sadowska |
| collection | DOAJ |
| description | Fungal extracts possess potential anticancer activity against many malignant neoplastic diseases. In this research, we focused on the evaluation of <i>Heterobasidion annosum</i> (HA) extract in colorectal cancer in an in vivo model. The mice with implanted DLD-1 human cancer cells were given HA extract, the referential drug—5-fluorouracil (5FU), or were treated with its combination. Thereafter, tumor volume was measured and apoptotic proteins such as caspase-8, caspase-3, p53, Bcl-2, and survivin were analyzed in mice serum with an ELISA assay. The Ki-67 protein was assessed in tumor cells by immunohistochemical examination. The biggest volumes of tumors were confirmed in the DLD-1 group, while the lowest were observed in the population treated with 5FU and/or HA extract. The assessment of apoptosis showed increased concentrations of caspase 8 and p53 protein after the combined administration of 5FU and HA extract. The levels of survivin and Bcl-2 were decreased in all tested groups compared to the DLD-1 group. Moreover, we observed a positive reaction for Ki-67 protein in all tested groups. Our findings confirm the apoptotic effect of extract given alone or with 5FU. The obtained results are innovative and provide a basis for further research concerning the antitumor activity of the HA extract, especially in the range of its interaction with an anticancer chemotherapeutic agent. |
| first_indexed | 2024-03-11T09:32:00Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T09:32:00Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0d1f02d3cce541b8a06fb4f4cfa200492023-11-16T17:31:15ZengMDPI AGMolecules1420-30492023-01-01283135210.3390/molecules28031352Beneficial Proapoptotic Effect of Heterobasidion Annosum Extract in Colorectal Cancer Xenograft Mouse ModelAnna Sadowska0Diana Sawicka1Katarzyna Godlewska2Katarzyna Guzińska-Ustymowicz3Ewa Zapora4Emilia Sokołowska5Halina Car6Department of Experimental Pharmacology, Medical University of Bialystok, Szpitalna 37, 15-295 Bialystok, PolandDepartment of Experimental Pharmacology, Medical University of Bialystok, Szpitalna 37, 15-295 Bialystok, PolandDepartment of Experimental Pharmacology, Medical University of Bialystok, Szpitalna 37, 15-295 Bialystok, PolandDepartment of General Pathomorphology, Medical University of Bialystok, Waszyngtona 13, 15-269 Bialystok, PolandDepartment of Silviculture and Forest Use, Institute of Forest Sciences, Bialystok University of Technology, Wiejska 45E, 15-351 Bialystok, PolandDepartment of Experimental Pharmacology, Medical University of Bialystok, Szpitalna 37, 15-295 Bialystok, PolandDepartment of Experimental Pharmacology, Medical University of Bialystok, Szpitalna 37, 15-295 Bialystok, PolandFungal extracts possess potential anticancer activity against many malignant neoplastic diseases. In this research, we focused on the evaluation of <i>Heterobasidion annosum</i> (HA) extract in colorectal cancer in an in vivo model. The mice with implanted DLD-1 human cancer cells were given HA extract, the referential drug—5-fluorouracil (5FU), or were treated with its combination. Thereafter, tumor volume was measured and apoptotic proteins such as caspase-8, caspase-3, p53, Bcl-2, and survivin were analyzed in mice serum with an ELISA assay. The Ki-67 protein was assessed in tumor cells by immunohistochemical examination. The biggest volumes of tumors were confirmed in the DLD-1 group, while the lowest were observed in the population treated with 5FU and/or HA extract. The assessment of apoptosis showed increased concentrations of caspase 8 and p53 protein after the combined administration of 5FU and HA extract. The levels of survivin and Bcl-2 were decreased in all tested groups compared to the DLD-1 group. Moreover, we observed a positive reaction for Ki-67 protein in all tested groups. Our findings confirm the apoptotic effect of extract given alone or with 5FU. The obtained results are innovative and provide a basis for further research concerning the antitumor activity of the HA extract, especially in the range of its interaction with an anticancer chemotherapeutic agent.https://www.mdpi.com/1420-3049/28/3/1352apoptosis<i>Heterobasidion annosum</i>colon cancer |
| spellingShingle | Anna Sadowska Diana Sawicka Katarzyna Godlewska Katarzyna Guzińska-Ustymowicz Ewa Zapora Emilia Sokołowska Halina Car Beneficial Proapoptotic Effect of Heterobasidion Annosum Extract in Colorectal Cancer Xenograft Mouse Model Molecules apoptosis <i>Heterobasidion annosum</i> colon cancer |
| title | Beneficial Proapoptotic Effect of Heterobasidion Annosum Extract in Colorectal Cancer Xenograft Mouse Model |
| title_full | Beneficial Proapoptotic Effect of Heterobasidion Annosum Extract in Colorectal Cancer Xenograft Mouse Model |
| title_fullStr | Beneficial Proapoptotic Effect of Heterobasidion Annosum Extract in Colorectal Cancer Xenograft Mouse Model |
| title_full_unstemmed | Beneficial Proapoptotic Effect of Heterobasidion Annosum Extract in Colorectal Cancer Xenograft Mouse Model |
| title_short | Beneficial Proapoptotic Effect of Heterobasidion Annosum Extract in Colorectal Cancer Xenograft Mouse Model |
| title_sort | beneficial proapoptotic effect of heterobasidion annosum extract in colorectal cancer xenograft mouse model |
| topic | apoptosis <i>Heterobasidion annosum</i> colon cancer |
| url | https://www.mdpi.com/1420-3049/28/3/1352 |
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