Metabolic pathway prediction of core microbiome based on enterotype and orotype
IntroductionIdentification of key microbiome components has been suggested to help address the maintenance of oral and intestinal health in humans. The core microbiome is similar in all individuals, whereas the diverse microbiome varies across individuals, based on their unique lifestyles and phenot...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1173085/full |
| _version_ | 1797797509898174464 |
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| author | Song Hee Lee Han Lee Hee Sang You Hee Sang You Ho-joong Sung Sung Hee Hyun |
| author_facet | Song Hee Lee Han Lee Hee Sang You Hee Sang You Ho-joong Sung Sung Hee Hyun |
| author_sort | Song Hee Lee |
| collection | DOAJ |
| description | IntroductionIdentification of key microbiome components has been suggested to help address the maintenance of oral and intestinal health in humans. The core microbiome is similar in all individuals, whereas the diverse microbiome varies across individuals, based on their unique lifestyles and phenotypic and genotypic determinants. In this study, we aimed to predict the metabolism of core microorganisms in the gut and oral environment based on enterotyping and orotyping.Materials and methodsGut and oral samples were collected from 83 Korean women aged 50 years or older. The extracted DNA was subjected to next-generation sequencing analysis of 16S rRNA hypervariable regions V3–V4.ResultsGut bacteria were clustered into three enterotypes, while oral bacteria were clustered into three orotypes. Sixty-three of the core microbiome between the gut and oral population were correlated, and different metabolic pathways were predicted for each type. Eubacterium_g11, Actinomyces, Atopobium, and Enterococcus were significantly positively correlated between the gut and oral abundance. The four bacteria were classified as type 3 in orotype and type 2 in enterotype.ConclusionOverall, the study suggested that collapsing the human body’s multidimensional microbiome into a few categories may help characterize the microbiomes better and address health issues more deeply. |
| first_indexed | 2024-03-13T03:50:26Z |
| format | Article |
| id | doaj.art-0d22237b2694401897981145f750c207 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-13T03:50:26Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-0d22237b2694401897981145f750c2072023-06-22T14:09:45ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-06-011310.3389/fcimb.2023.11730851173085Metabolic pathway prediction of core microbiome based on enterotype and orotypeSong Hee Lee0Han Lee1Hee Sang You2Hee Sang You3Ho-joong Sung4Sung Hee Hyun5Department of Biomedical Laboratory Science, Graduate School, Eulji University, Uijeongbu, Republic of KoreaDepartment of Biomedical Laboratory Science, Graduate School, Eulji University, Uijeongbu, Republic of KoreaLaboratory of Gastrointestinal Mucosal Immunology, Chung-Ang University College of Medicine, Seoul, Republic of KoreaDepartment of Internal Medicine, Chung-Ang University College of Medicine, Seoul, Republic of KoreaDepartment of Biomedical Laboratory Science, College of Health Sciences, Eulji University, Seongnam, Republic of KoreaDepartment of Biomedical Laboratory Science, Graduate School, Eulji University, Uijeongbu, Republic of KoreaIntroductionIdentification of key microbiome components has been suggested to help address the maintenance of oral and intestinal health in humans. The core microbiome is similar in all individuals, whereas the diverse microbiome varies across individuals, based on their unique lifestyles and phenotypic and genotypic determinants. In this study, we aimed to predict the metabolism of core microorganisms in the gut and oral environment based on enterotyping and orotyping.Materials and methodsGut and oral samples were collected from 83 Korean women aged 50 years or older. The extracted DNA was subjected to next-generation sequencing analysis of 16S rRNA hypervariable regions V3–V4.ResultsGut bacteria were clustered into three enterotypes, while oral bacteria were clustered into three orotypes. Sixty-three of the core microbiome between the gut and oral population were correlated, and different metabolic pathways were predicted for each type. Eubacterium_g11, Actinomyces, Atopobium, and Enterococcus were significantly positively correlated between the gut and oral abundance. The four bacteria were classified as type 3 in orotype and type 2 in enterotype.ConclusionOverall, the study suggested that collapsing the human body’s multidimensional microbiome into a few categories may help characterize the microbiomes better and address health issues more deeply.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1173085/fulloral-gut axisoral microbiomegut microbiomecore microbiomeEubacterium_g11Actinomyces |
| spellingShingle | Song Hee Lee Han Lee Hee Sang You Hee Sang You Ho-joong Sung Sung Hee Hyun Metabolic pathway prediction of core microbiome based on enterotype and orotype Frontiers in Cellular and Infection Microbiology oral-gut axis oral microbiome gut microbiome core microbiome Eubacterium_g11 Actinomyces |
| title | Metabolic pathway prediction of core microbiome based on enterotype and orotype |
| title_full | Metabolic pathway prediction of core microbiome based on enterotype and orotype |
| title_fullStr | Metabolic pathway prediction of core microbiome based on enterotype and orotype |
| title_full_unstemmed | Metabolic pathway prediction of core microbiome based on enterotype and orotype |
| title_short | Metabolic pathway prediction of core microbiome based on enterotype and orotype |
| title_sort | metabolic pathway prediction of core microbiome based on enterotype and orotype |
| topic | oral-gut axis oral microbiome gut microbiome core microbiome Eubacterium_g11 Actinomyces |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1173085/full |
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