Identification of <i>JPX-RABEP1</i> Pair as an Immune-Related Biomarker and Therapeutic Target in Pulmonary Arterial Hypertension by Bioinformatics and Experimental Analyses
Pulmonary arterial hypertension (PAH) is a pulmonary vascular disease characterized by pulmonary vascular remodeling and right heart enlargement the pathogenesis of PAH is complicated; no biologic-based therapy is available for the treatment of PAH, but recent studies suggest that inflammatory respo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/24/15559 |
| _version_ | 1797457307677753344 |
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| author | Qian Gong Zhewei Hu Qiao Jin Yan Yan Yan Liu Jin He Lenan Zhuang Huanan Wang |
| author_facet | Qian Gong Zhewei Hu Qiao Jin Yan Yan Yan Liu Jin He Lenan Zhuang Huanan Wang |
| author_sort | Qian Gong |
| collection | DOAJ |
| description | Pulmonary arterial hypertension (PAH) is a pulmonary vascular disease characterized by pulmonary vascular remodeling and right heart enlargement the pathogenesis of PAH is complicated; no biologic-based therapy is available for the treatment of PAH, but recent studies suggest that inflammatory response and abnormal proliferation of pulmonary artery smooth muscle cells are the main pathogenic mechanism, while the role of immune-related long non-coding RNAs (lncRNAs) remains unclear. The aim of this study was to systematically analyze immune-related lncRNAs in PAH. Here, we downloaded a publicly available microarray data from PAH and control patients (GSE113439). A total of 243 up-regulated and 203 down-regulated differentially expressed genes (DEGs) were screened, and immune-related DEGs were further obtained from ImmPort. The immune-related lncRNAs were obtained by co-expression analysis of immune-related mRNAs. Then, immune-related lncRNAs-mRNAs network including 2 lncRNAs and 6 mRNAs was constructed which share regulatory miRNAs and have significant correlation. Among the lncRNA-mRNA pairs, one pair (<i>JPX-RABEP1</i>) was verified in the validating dataset GSE53408 and PAH mouse model. Furthermore, the immune cell infiltration analysis of the GSE113439 dataset revealed that the <i>JPX-RABEP1</i> pair may participate in the occurrence and development of PAH through immune cell infiltration. Together, our findings reveal that the lncRNA-mRNA pair <i>JPX-RABEP1</i> may be a novel biomarker and therapeutic target for PAH. |
| first_indexed | 2024-03-09T16:20:21Z |
| format | Article |
| id | doaj.art-0d2747347bce4e08b250c72acb1274ac |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:20:21Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0d2747347bce4e08b250c72acb1274ac2023-11-24T15:23:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241555910.3390/ijms232415559Identification of <i>JPX-RABEP1</i> Pair as an Immune-Related Biomarker and Therapeutic Target in Pulmonary Arterial Hypertension by Bioinformatics and Experimental AnalysesQian Gong0Zhewei Hu1Qiao Jin2Yan Yan3Yan Liu4Jin He5Lenan Zhuang6Huanan Wang7Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Genetics and Reproduction, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, ChinaDepartment of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaPulmonary arterial hypertension (PAH) is a pulmonary vascular disease characterized by pulmonary vascular remodeling and right heart enlargement the pathogenesis of PAH is complicated; no biologic-based therapy is available for the treatment of PAH, but recent studies suggest that inflammatory response and abnormal proliferation of pulmonary artery smooth muscle cells are the main pathogenic mechanism, while the role of immune-related long non-coding RNAs (lncRNAs) remains unclear. The aim of this study was to systematically analyze immune-related lncRNAs in PAH. Here, we downloaded a publicly available microarray data from PAH and control patients (GSE113439). A total of 243 up-regulated and 203 down-regulated differentially expressed genes (DEGs) were screened, and immune-related DEGs were further obtained from ImmPort. The immune-related lncRNAs were obtained by co-expression analysis of immune-related mRNAs. Then, immune-related lncRNAs-mRNAs network including 2 lncRNAs and 6 mRNAs was constructed which share regulatory miRNAs and have significant correlation. Among the lncRNA-mRNA pairs, one pair (<i>JPX-RABEP1</i>) was verified in the validating dataset GSE53408 and PAH mouse model. Furthermore, the immune cell infiltration analysis of the GSE113439 dataset revealed that the <i>JPX-RABEP1</i> pair may participate in the occurrence and development of PAH through immune cell infiltration. Together, our findings reveal that the lncRNA-mRNA pair <i>JPX-RABEP1</i> may be a novel biomarker and therapeutic target for PAH.https://www.mdpi.com/1422-0067/23/24/15559bioinformatics analysispulmonary arterial hypertensionnon-coding RNAsimmune cell infiltration |
| spellingShingle | Qian Gong Zhewei Hu Qiao Jin Yan Yan Yan Liu Jin He Lenan Zhuang Huanan Wang Identification of <i>JPX-RABEP1</i> Pair as an Immune-Related Biomarker and Therapeutic Target in Pulmonary Arterial Hypertension by Bioinformatics and Experimental Analyses International Journal of Molecular Sciences bioinformatics analysis pulmonary arterial hypertension non-coding RNAs immune cell infiltration |
| title | Identification of <i>JPX-RABEP1</i> Pair as an Immune-Related Biomarker and Therapeutic Target in Pulmonary Arterial Hypertension by Bioinformatics and Experimental Analyses |
| title_full | Identification of <i>JPX-RABEP1</i> Pair as an Immune-Related Biomarker and Therapeutic Target in Pulmonary Arterial Hypertension by Bioinformatics and Experimental Analyses |
| title_fullStr | Identification of <i>JPX-RABEP1</i> Pair as an Immune-Related Biomarker and Therapeutic Target in Pulmonary Arterial Hypertension by Bioinformatics and Experimental Analyses |
| title_full_unstemmed | Identification of <i>JPX-RABEP1</i> Pair as an Immune-Related Biomarker and Therapeutic Target in Pulmonary Arterial Hypertension by Bioinformatics and Experimental Analyses |
| title_short | Identification of <i>JPX-RABEP1</i> Pair as an Immune-Related Biomarker and Therapeutic Target in Pulmonary Arterial Hypertension by Bioinformatics and Experimental Analyses |
| title_sort | identification of i jpx rabep1 i pair as an immune related biomarker and therapeutic target in pulmonary arterial hypertension by bioinformatics and experimental analyses |
| topic | bioinformatics analysis pulmonary arterial hypertension non-coding RNAs immune cell infiltration |
| url | https://www.mdpi.com/1422-0067/23/24/15559 |
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