Alginate/chitosan/diallyl disulfide nanoparticles: Synthesis, characterization and their anti-inflammatory efficacy on TPA-induced acute mouse ear inflammation
Diallyl disulfide (DADS) is a garlic (Allium sativum) derived potent anti-inflammatory compound. Due to poor bioavailability, its biological application has limitations. Nanoencapsulation of therapeutic drugs is a promising approach to increase their bioavailability, specificity, and efficacy. In ou...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266689392300083X |
| _version_ | 1797383633656348672 |
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| author | Abhipriya Chatterjee Sampurna Bhattacharya Runa Sur |
| author_facet | Abhipriya Chatterjee Sampurna Bhattacharya Runa Sur |
| author_sort | Abhipriya Chatterjee |
| collection | DOAJ |
| description | Diallyl disulfide (DADS) is a garlic (Allium sativum) derived potent anti-inflammatory compound. Due to poor bioavailability, its biological application has limitations. Nanoencapsulation of therapeutic drugs is a promising approach to increase their bioavailability, specificity, and efficacy. In our approach, DADS was coated with the natural polymers alginate and chitosan into a polyelectrolytic nano-formulation. Characterization of the nanoparticles showed monodispersed nanosized particles and a release study at pH 5.5 revealed sustained release of the drug. To investigate the anti-inflammatory efficacy of the DADS nanoparticle (DADS-NP), a comparative study with the free drug was conducted on a mouse model of 12-O-tetradecanoylphorbol-13-acetate (TPA) induced ear edema. The results indicated that DADS-NP significantly alleviated TPA-induced ear edema more effectively than the free drug of the same concentration. Further, mechanistic studies indicated that DADS-NP more efficiently downregulated the phosphorylation of p38 mitogen activated protein kinase which subsequently restricted the activation and nuclear translocation of downstream transcription factor nuclear factor- кB (NF-кB). This downregulated the expression of pro-inflammatory proteins cyclooxygenase-2 (COX-2) and inducible-nitric oxide synthase (i-NOS) thus mitigating ear edema. Cellular internalization of the DADS-NP was observed. Collectively, our studies indicate enhanced anti-inflammatory efficiency of DADS-NP when compared to free DADS suggesting enhanced stability and bioavailability. |
| first_indexed | 2024-03-08T21:23:53Z |
| format | Article |
| id | doaj.art-0d27d895ef7145368d016b320d365ac6 |
| institution | Directory Open Access Journal |
| issn | 2666-8939 |
| language | English |
| last_indexed | 2024-03-08T21:23:53Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj.art-0d27d895ef7145368d016b320d365ac62023-12-21T07:37:43ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392023-12-016100362Alginate/chitosan/diallyl disulfide nanoparticles: Synthesis, characterization and their anti-inflammatory efficacy on TPA-induced acute mouse ear inflammationAbhipriya Chatterjee0Sampurna Bhattacharya1Runa Sur2Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, Kolkata, IndiaDepartment of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, Kolkata, IndiaCorresponding author at: Department of Biophysics, Molecular Biology and Bioinformatics, 92 A.P.C. Road, University of Calcutta, Kolkata-700009, India.; Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, Kolkata, IndiaDiallyl disulfide (DADS) is a garlic (Allium sativum) derived potent anti-inflammatory compound. Due to poor bioavailability, its biological application has limitations. Nanoencapsulation of therapeutic drugs is a promising approach to increase their bioavailability, specificity, and efficacy. In our approach, DADS was coated with the natural polymers alginate and chitosan into a polyelectrolytic nano-formulation. Characterization of the nanoparticles showed monodispersed nanosized particles and a release study at pH 5.5 revealed sustained release of the drug. To investigate the anti-inflammatory efficacy of the DADS nanoparticle (DADS-NP), a comparative study with the free drug was conducted on a mouse model of 12-O-tetradecanoylphorbol-13-acetate (TPA) induced ear edema. The results indicated that DADS-NP significantly alleviated TPA-induced ear edema more effectively than the free drug of the same concentration. Further, mechanistic studies indicated that DADS-NP more efficiently downregulated the phosphorylation of p38 mitogen activated protein kinase which subsequently restricted the activation and nuclear translocation of downstream transcription factor nuclear factor- кB (NF-кB). This downregulated the expression of pro-inflammatory proteins cyclooxygenase-2 (COX-2) and inducible-nitric oxide synthase (i-NOS) thus mitigating ear edema. Cellular internalization of the DADS-NP was observed. Collectively, our studies indicate enhanced anti-inflammatory efficiency of DADS-NP when compared to free DADS suggesting enhanced stability and bioavailability.http://www.sciencedirect.com/science/article/pii/S266689392300083XDiallyl disulfideBiopolymer core-shell nanoparticleEar edemaNF-кBEpidermal hyperplasiaMitogen activated protein kinase |
| spellingShingle | Abhipriya Chatterjee Sampurna Bhattacharya Runa Sur Alginate/chitosan/diallyl disulfide nanoparticles: Synthesis, characterization and their anti-inflammatory efficacy on TPA-induced acute mouse ear inflammation Carbohydrate Polymer Technologies and Applications Diallyl disulfide Biopolymer core-shell nanoparticle Ear edema NF-кB Epidermal hyperplasia Mitogen activated protein kinase |
| title | Alginate/chitosan/diallyl disulfide nanoparticles: Synthesis, characterization and their anti-inflammatory efficacy on TPA-induced acute mouse ear inflammation |
| title_full | Alginate/chitosan/diallyl disulfide nanoparticles: Synthesis, characterization and their anti-inflammatory efficacy on TPA-induced acute mouse ear inflammation |
| title_fullStr | Alginate/chitosan/diallyl disulfide nanoparticles: Synthesis, characterization and their anti-inflammatory efficacy on TPA-induced acute mouse ear inflammation |
| title_full_unstemmed | Alginate/chitosan/diallyl disulfide nanoparticles: Synthesis, characterization and their anti-inflammatory efficacy on TPA-induced acute mouse ear inflammation |
| title_short | Alginate/chitosan/diallyl disulfide nanoparticles: Synthesis, characterization and their anti-inflammatory efficacy on TPA-induced acute mouse ear inflammation |
| title_sort | alginate chitosan diallyl disulfide nanoparticles synthesis characterization and their anti inflammatory efficacy on tpa induced acute mouse ear inflammation |
| topic | Diallyl disulfide Biopolymer core-shell nanoparticle Ear edema NF-кB Epidermal hyperplasia Mitogen activated protein kinase |
| url | http://www.sciencedirect.com/science/article/pii/S266689392300083X |
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