Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals’ Bone Marrow
In this work, proteins and peptides were isolated from four kinds of animal bone marrow and characterized by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and liquid chromatography-mass spectro...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-07-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/23/7/1673 |
| _version_ | 1828241780448952320 |
|---|---|
| author | Parhat Rozi Parida Maimaiti Aytursun Abuduwaili Ahmidin Wali Abulimiti Yili Haji Akber Aisa |
| author_facet | Parhat Rozi Parida Maimaiti Aytursun Abuduwaili Ahmidin Wali Abulimiti Yili Haji Akber Aisa |
| author_sort | Parhat Rozi |
| collection | DOAJ |
| description | In this work, proteins and peptides were isolated from four kinds of animal bone marrow and characterized by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and liquid chromatography-mass spectrometry (LC/MS). The antimicrobial and antioxidant activity of these proteins were investigated in vitro. The nutritional value was evaluated by analyzing their free amino acid composition. The results indicates that all of the extracts appeared two bands at SDS-PAGE, the peptide band at 4.1–10 kDa and protein band at 66 kDa, these data are consistent with LC/MS results. FT-IR analysis showed that the secondary structure of protein mainly consists of α-helix. SEM micrographs revealed that the fractions have different morphological characteristics. Horse bone marrow protein (HBMP) showed the highest antioxidant activity to DPPH free radical, IC50 value was 0.573 mg/mL. Most of the obtained fractions showed antimicrobial activities towards Escherichia coli (EC) and Candida albicans (CA). Total free amino acid content ranged between 5.15–49.60 mg/g, and among them, HBMP displayed the highest abundance, 49.7 mg/g, which amino acid composition ratio approached the Food and Agriculture Organization/World Health Organization (FAO/WHO) ideal amino acid pattern recommendation. This study provides fundamental knowledge and a basic study method for the research into and development of animal bone marrow proteins and peptides as functional food and drug resources. |
| first_indexed | 2024-04-12T22:05:59Z |
| format | Article |
| id | doaj.art-0d280c36093c4b05b17aaf0f075e5f74 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T22:05:59Z |
| publishDate | 2018-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0d280c36093c4b05b17aaf0f075e5f742022-12-22T03:14:56ZengMDPI AGMolecules1420-30492018-07-01237167310.3390/molecules23071673molecules23071673Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals’ Bone MarrowParhat Rozi0Parida Maimaiti1Aytursun Abuduwaili2Ahmidin Wali3Abulimiti Yili4Haji Akber Aisa5The Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No 40-1 Road, Urumqi 830011, ChinaDepartment of Nursing, Xinjiang Medical University, Urumqi 830011, ChinaThe Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No 40-1 Road, Urumqi 830011, ChinaThe Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No 40-1 Road, Urumqi 830011, ChinaThe Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No 40-1 Road, Urumqi 830011, ChinaThe Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No 40-1 Road, Urumqi 830011, ChinaIn this work, proteins and peptides were isolated from four kinds of animal bone marrow and characterized by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and liquid chromatography-mass spectrometry (LC/MS). The antimicrobial and antioxidant activity of these proteins were investigated in vitro. The nutritional value was evaluated by analyzing their free amino acid composition. The results indicates that all of the extracts appeared two bands at SDS-PAGE, the peptide band at 4.1–10 kDa and protein band at 66 kDa, these data are consistent with LC/MS results. FT-IR analysis showed that the secondary structure of protein mainly consists of α-helix. SEM micrographs revealed that the fractions have different morphological characteristics. Horse bone marrow protein (HBMP) showed the highest antioxidant activity to DPPH free radical, IC50 value was 0.573 mg/mL. Most of the obtained fractions showed antimicrobial activities towards Escherichia coli (EC) and Candida albicans (CA). Total free amino acid content ranged between 5.15–49.60 mg/g, and among them, HBMP displayed the highest abundance, 49.7 mg/g, which amino acid composition ratio approached the Food and Agriculture Organization/World Health Organization (FAO/WHO) ideal amino acid pattern recommendation. This study provides fundamental knowledge and a basic study method for the research into and development of animal bone marrow proteins and peptides as functional food and drug resources.http://www.mdpi.com/1420-3049/23/7/1673bone marrowprotein and peptidenutritional evaluationSDS-PAGELC/MS |
| spellingShingle | Parhat Rozi Parida Maimaiti Aytursun Abuduwaili Ahmidin Wali Abulimiti Yili Haji Akber Aisa Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals’ Bone Marrow Molecules bone marrow protein and peptide nutritional evaluation SDS-PAGE LC/MS |
| title | Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals’ Bone Marrow |
| title_full | Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals’ Bone Marrow |
| title_fullStr | Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals’ Bone Marrow |
| title_full_unstemmed | Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals’ Bone Marrow |
| title_short | Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals’ Bone Marrow |
| title_sort | isolation and evaluation of bioactive protein and peptide from domestic animals bone marrow |
| topic | bone marrow protein and peptide nutritional evaluation SDS-PAGE LC/MS |
| url | http://www.mdpi.com/1420-3049/23/7/1673 |
| work_keys_str_mv | AT parhatrozi isolationandevaluationofbioactiveproteinandpeptidefromdomesticanimalsbonemarrow AT paridamaimaiti isolationandevaluationofbioactiveproteinandpeptidefromdomesticanimalsbonemarrow AT aytursunabuduwaili isolationandevaluationofbioactiveproteinandpeptidefromdomesticanimalsbonemarrow AT ahmidinwali isolationandevaluationofbioactiveproteinandpeptidefromdomesticanimalsbonemarrow AT abulimitiyili isolationandevaluationofbioactiveproteinandpeptidefromdomesticanimalsbonemarrow AT hajiakberaisa isolationandevaluationofbioactiveproteinandpeptidefromdomesticanimalsbonemarrow |