Recombinant Rift Valley fever viruses encoding bluetongue virus (BTV) antigens: Immunity and efficacy studies upon a BTV-4 challenge.
<h4>Background</h4>Many ruminant diseases of viral aetiology can be effectively prevented using appropriate vaccination measures. For diseases such as Rift Valley fever (RVF) the long inter-epizootic periods make routine vaccination programs unfeasible. Coupling RVF prophylaxis with seas...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-12-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0008942 |
| _version_ | 1819140384085770240 |
|---|---|
| author | Sandra Moreno Eva Calvo-Pinilla Stephanie Devignot Friedemann Weber Javier Ortego Alejandro Brun |
| author_facet | Sandra Moreno Eva Calvo-Pinilla Stephanie Devignot Friedemann Weber Javier Ortego Alejandro Brun |
| author_sort | Sandra Moreno |
| collection | DOAJ |
| description | <h4>Background</h4>Many ruminant diseases of viral aetiology can be effectively prevented using appropriate vaccination measures. For diseases such as Rift Valley fever (RVF) the long inter-epizootic periods make routine vaccination programs unfeasible. Coupling RVF prophylaxis with seasonal vaccination programmes by means of multivalent vaccine platforms would help to reduce the risk of new RVF outbreaks.<h4>Methodology/principal findings</h4>In this work we generated recombinant attenuated Rift Valley fever viruses (RVFVs) encoding in place of the virulence factor NSs either the VP2 capsid protein or a truncated form of the non-structural NS1 protein of bluetongue virus serotype 4 (BTV-4). The recombinant viruses were able to carry and express the heterologous BTV genes upon consecutive passages in cell cultures. In murine models, a single immunization was sufficient to protect mice upon RVFV challenge and to elicit a specific immune response against BTV-4 antigens that was fully protective after a BTV-4 boost. In sheep, a natural host for RVFV and BTV, both vaccines proved immunogenic although conferred only partial protection after a virulent BTV-4 reassortant Morocco strain challenge.<h4>Conclusions/significance</h4>Though additional optimization will be needed to improve the efficacy data against BTV in sheep, our findings warrant further developments of attenuated RVFV as a dual vaccine platform carrying heterologous immune relevant antigens for ruminant diseases in RVF risk areas. |
| first_indexed | 2024-12-22T11:37:42Z |
| format | Article |
| id | doaj.art-0d28133f9c2d47e7a52506c3c3ae526c |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-22T11:37:42Z |
| publishDate | 2020-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-0d28133f9c2d47e7a52506c3c3ae526c2022-12-21T18:27:22ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352020-12-011412e000894210.1371/journal.pntd.0008942Recombinant Rift Valley fever viruses encoding bluetongue virus (BTV) antigens: Immunity and efficacy studies upon a BTV-4 challenge.Sandra MorenoEva Calvo-PinillaStephanie DevignotFriedemann WeberJavier OrtegoAlejandro Brun<h4>Background</h4>Many ruminant diseases of viral aetiology can be effectively prevented using appropriate vaccination measures. For diseases such as Rift Valley fever (RVF) the long inter-epizootic periods make routine vaccination programs unfeasible. Coupling RVF prophylaxis with seasonal vaccination programmes by means of multivalent vaccine platforms would help to reduce the risk of new RVF outbreaks.<h4>Methodology/principal findings</h4>In this work we generated recombinant attenuated Rift Valley fever viruses (RVFVs) encoding in place of the virulence factor NSs either the VP2 capsid protein or a truncated form of the non-structural NS1 protein of bluetongue virus serotype 4 (BTV-4). The recombinant viruses were able to carry and express the heterologous BTV genes upon consecutive passages in cell cultures. In murine models, a single immunization was sufficient to protect mice upon RVFV challenge and to elicit a specific immune response against BTV-4 antigens that was fully protective after a BTV-4 boost. In sheep, a natural host for RVFV and BTV, both vaccines proved immunogenic although conferred only partial protection after a virulent BTV-4 reassortant Morocco strain challenge.<h4>Conclusions/significance</h4>Though additional optimization will be needed to improve the efficacy data against BTV in sheep, our findings warrant further developments of attenuated RVFV as a dual vaccine platform carrying heterologous immune relevant antigens for ruminant diseases in RVF risk areas.https://doi.org/10.1371/journal.pntd.0008942 |
| spellingShingle | Sandra Moreno Eva Calvo-Pinilla Stephanie Devignot Friedemann Weber Javier Ortego Alejandro Brun Recombinant Rift Valley fever viruses encoding bluetongue virus (BTV) antigens: Immunity and efficacy studies upon a BTV-4 challenge. PLoS Neglected Tropical Diseases |
| title | Recombinant Rift Valley fever viruses encoding bluetongue virus (BTV) antigens: Immunity and efficacy studies upon a BTV-4 challenge. |
| title_full | Recombinant Rift Valley fever viruses encoding bluetongue virus (BTV) antigens: Immunity and efficacy studies upon a BTV-4 challenge. |
| title_fullStr | Recombinant Rift Valley fever viruses encoding bluetongue virus (BTV) antigens: Immunity and efficacy studies upon a BTV-4 challenge. |
| title_full_unstemmed | Recombinant Rift Valley fever viruses encoding bluetongue virus (BTV) antigens: Immunity and efficacy studies upon a BTV-4 challenge. |
| title_short | Recombinant Rift Valley fever viruses encoding bluetongue virus (BTV) antigens: Immunity and efficacy studies upon a BTV-4 challenge. |
| title_sort | recombinant rift valley fever viruses encoding bluetongue virus btv antigens immunity and efficacy studies upon a btv 4 challenge |
| url | https://doi.org/10.1371/journal.pntd.0008942 |
| work_keys_str_mv | AT sandramoreno recombinantriftvalleyfevervirusesencodingbluetonguevirusbtvantigensimmunityandefficacystudiesuponabtv4challenge AT evacalvopinilla recombinantriftvalleyfevervirusesencodingbluetonguevirusbtvantigensimmunityandefficacystudiesuponabtv4challenge AT stephaniedevignot recombinantriftvalleyfevervirusesencodingbluetonguevirusbtvantigensimmunityandefficacystudiesuponabtv4challenge AT friedemannweber recombinantriftvalleyfevervirusesencodingbluetonguevirusbtvantigensimmunityandefficacystudiesuponabtv4challenge AT javierortego recombinantriftvalleyfevervirusesencodingbluetonguevirusbtvantigensimmunityandefficacystudiesuponabtv4challenge AT alejandrobrun recombinantriftvalleyfevervirusesencodingbluetonguevirusbtvantigensimmunityandefficacystudiesuponabtv4challenge |