Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit
Background: Hemocompatibility of left ventricular assist devices is essential for preventing adverse events. In this study, we compared the hemocompatibility of an axial-flow (Sputnik) to a centrifugal-flow (HeartMate 3) pump. Methods: Both pumps were integrated into identical in vitro test circuits...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/12/3431 |
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| author | Patrick Borchers Patrick Winnersbach Sandra Kraemer Christian Beckers Eva Miriam Buhl Steffen Leonhardt Rolf Rossaint Marian Walter Thomas Breuer Christian Bleilevens |
| author_facet | Patrick Borchers Patrick Winnersbach Sandra Kraemer Christian Beckers Eva Miriam Buhl Steffen Leonhardt Rolf Rossaint Marian Walter Thomas Breuer Christian Bleilevens |
| author_sort | Patrick Borchers |
| collection | DOAJ |
| description | Background: Hemocompatibility of left ventricular assist devices is essential for preventing adverse events. In this study, we compared the hemocompatibility of an axial-flow (Sputnik) to a centrifugal-flow (HeartMate 3) pump. Methods: Both pumps were integrated into identical in vitro test circuits, each filled with 75 mL heparinized human blood of the same donor. During each experiment (n = 7), the pumps were operated with equal flow for six hours. Blood sampling and analysis were performed on a regular schedule. The analytes were indicators of hemolysis, coagulation activation, platelet count and activation, as well as extracellular vesicles. Results: Sputnik induced higher hemolysis compared to the HeartMate 3 after 360 min. Furthermore, platelet activation was higher for Sputnik after 120 min onward. In the HeartMate 3 circuit, the platelet count was reduced within the first hour. Furthermore, Sputnik triggered a more pronounced increase in extracellular vesicles, a potential trigger for adverse events in left ventricular assist device application. Activation of coagulation showed a time-dependent increase, with no differences between both groups. Conclusions: This experimental study confirms the hypothesis that axial-flow pumps may induce stronger hemolysis compared to centrifugal pumps, coming along with larger amounts of circulating extracellular vesicles and a stronger PLT activation. |
| first_indexed | 2024-03-09T23:26:58Z |
| format | Article |
| id | doaj.art-0d286ba855e6465182beee582f25b77c |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T23:26:58Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
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| series | Journal of Clinical Medicine |
| spelling | doaj.art-0d286ba855e6465182beee582f25b77c2023-11-23T17:15:55ZengMDPI AGJournal of Clinical Medicine2077-03832022-06-011112343110.3390/jcm11123431Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test CircuitPatrick Borchers0Patrick Winnersbach1Sandra Kraemer2Christian Beckers3Eva Miriam Buhl4Steffen Leonhardt5Rolf Rossaint6Marian Walter7Thomas Breuer8Christian Bleilevens9Medical Information Technology, RWTH Aachen University, Pauwelsstraße 20, 52074 Aachen, GermanyDepartment of Anesthesiology, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074 Aachen, GermanyDepartment of Intensive Care and Intermediate Care, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074 Aachen, GermanyDepartment of Intensive Care and Intermediate Care, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074 Aachen, GermanyInstitute of Pathology, Electron Microscopy Facility, RWTH Aachen University, Pauwelsstraße 30, 52074 Aachen, GermanyMedical Information Technology, RWTH Aachen University, Pauwelsstraße 20, 52074 Aachen, GermanyDepartment of Anesthesiology, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074 Aachen, GermanyMedical Information Technology, RWTH Aachen University, Pauwelsstraße 20, 52074 Aachen, GermanyDepartment of Intensive Care and Intermediate Care, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074 Aachen, GermanyDepartment of Anesthesiology, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074 Aachen, GermanyBackground: Hemocompatibility of left ventricular assist devices is essential for preventing adverse events. In this study, we compared the hemocompatibility of an axial-flow (Sputnik) to a centrifugal-flow (HeartMate 3) pump. Methods: Both pumps were integrated into identical in vitro test circuits, each filled with 75 mL heparinized human blood of the same donor. During each experiment (n = 7), the pumps were operated with equal flow for six hours. Blood sampling and analysis were performed on a regular schedule. The analytes were indicators of hemolysis, coagulation activation, platelet count and activation, as well as extracellular vesicles. Results: Sputnik induced higher hemolysis compared to the HeartMate 3 after 360 min. Furthermore, platelet activation was higher for Sputnik after 120 min onward. In the HeartMate 3 circuit, the platelet count was reduced within the first hour. Furthermore, Sputnik triggered a more pronounced increase in extracellular vesicles, a potential trigger for adverse events in left ventricular assist device application. Activation of coagulation showed a time-dependent increase, with no differences between both groups. Conclusions: This experimental study confirms the hypothesis that axial-flow pumps may induce stronger hemolysis compared to centrifugal pumps, coming along with larger amounts of circulating extracellular vesicles and a stronger PLT activation.https://www.mdpi.com/2077-0383/11/12/3431left ventricular assist devicein vitro test circuitSputnikHeartMate 3hemocompatibilityhemolysis |
| spellingShingle | Patrick Borchers Patrick Winnersbach Sandra Kraemer Christian Beckers Eva Miriam Buhl Steffen Leonhardt Rolf Rossaint Marian Walter Thomas Breuer Christian Bleilevens Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit Journal of Clinical Medicine left ventricular assist device in vitro test circuit Sputnik HeartMate 3 hemocompatibility hemolysis |
| title | Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit |
| title_full | Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit |
| title_fullStr | Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit |
| title_full_unstemmed | Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit |
| title_short | Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit |
| title_sort | comparison of the hemocompatibility of an axial and a centrifugal left ventricular assist device in an in vitro test circuit |
| topic | left ventricular assist device in vitro test circuit Sputnik HeartMate 3 hemocompatibility hemolysis |
| url | https://www.mdpi.com/2077-0383/11/12/3431 |
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