Methods for Three-Dimensional All-Optical Manipulation of Neural Circuits
Optical means for modulating and monitoring neuronal activity, have provided substantial insights to neurophysiology and toward our understanding of how the brain works. Optogenetic actuators, calcium or voltage imaging probes and other molecular tools, combined with advanced microscopies have allow...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fncel.2018.00469/full |
| _version_ | 1811268817646518272 |
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| author | Emiliano Ronzitti Valentina Emiliani Eirini Papagiakoumou |
| author_facet | Emiliano Ronzitti Valentina Emiliani Eirini Papagiakoumou |
| author_sort | Emiliano Ronzitti |
| collection | DOAJ |
| description | Optical means for modulating and monitoring neuronal activity, have provided substantial insights to neurophysiology and toward our understanding of how the brain works. Optogenetic actuators, calcium or voltage imaging probes and other molecular tools, combined with advanced microscopies have allowed an “all-optical” readout and modulation of neural circuits. Completion of this remarkable work is evolving toward a three-dimensional (3D) manipulation of neural ensembles at a high spatiotemporal resolution. Recently, original optical methods have been proposed for both activating and monitoring neurons in a 3D space, mainly through optogenetic compounds. Here, we review these methods and anticipate possible combinations among them. |
| first_indexed | 2024-04-12T21:29:43Z |
| format | Article |
| id | doaj.art-0d29a85ce7f64985988554d8dc1c87fb |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-04-12T21:29:43Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-0d29a85ce7f64985988554d8dc1c87fb2022-12-22T03:16:04ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022018-12-011210.3389/fncel.2018.00469423174Methods for Three-Dimensional All-Optical Manipulation of Neural CircuitsEmiliano RonzittiValentina EmilianiEirini PapagiakoumouOptical means for modulating and monitoring neuronal activity, have provided substantial insights to neurophysiology and toward our understanding of how the brain works. Optogenetic actuators, calcium or voltage imaging probes and other molecular tools, combined with advanced microscopies have allowed an “all-optical” readout and modulation of neural circuits. Completion of this remarkable work is evolving toward a three-dimensional (3D) manipulation of neural ensembles at a high spatiotemporal resolution. Recently, original optical methods have been proposed for both activating and monitoring neurons in a 3D space, mainly through optogenetic compounds. Here, we review these methods and anticipate possible combinations among them.https://www.frontiersin.org/article/10.3389/fncel.2018.00469/fulllight-shapingthree-dimensional photostimulationthree-dimensional functional imagingall-optical neuronal studiesoptogeneticsneural circuits |
| spellingShingle | Emiliano Ronzitti Valentina Emiliani Eirini Papagiakoumou Methods for Three-Dimensional All-Optical Manipulation of Neural Circuits Frontiers in Cellular Neuroscience light-shaping three-dimensional photostimulation three-dimensional functional imaging all-optical neuronal studies optogenetics neural circuits |
| title | Methods for Three-Dimensional All-Optical Manipulation of Neural Circuits |
| title_full | Methods for Three-Dimensional All-Optical Manipulation of Neural Circuits |
| title_fullStr | Methods for Three-Dimensional All-Optical Manipulation of Neural Circuits |
| title_full_unstemmed | Methods for Three-Dimensional All-Optical Manipulation of Neural Circuits |
| title_short | Methods for Three-Dimensional All-Optical Manipulation of Neural Circuits |
| title_sort | methods for three dimensional all optical manipulation of neural circuits |
| topic | light-shaping three-dimensional photostimulation three-dimensional functional imaging all-optical neuronal studies optogenetics neural circuits |
| url | https://www.frontiersin.org/article/10.3389/fncel.2018.00469/full |
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