Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China
<p>Abstract</p> <p>Background</p> <p>Bovine adenovirus type 3 (BAV-3) belongs to the <it>Mastadenovirus </it>genus of the family <it>Adenoviridae </it>and is involved in respiratory and enteric infections of calves. The isolation of BAV-3 has not...
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| Format: | Article |
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BMC
2011-12-01
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| Series: | Virology Journal |
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| Online Access: | http://www.virologyj.com/content/8/1/557 |
| _version_ | 1818551628572131328 |
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| author | Zhu Yuan-Mao Yu Zuo Cai Hong Gao Yu-Ran Dong Xiu-Mei Li Zhao-Li Shi Hong-Fei Meng Qing-Feng Lu Chuang Xue Fei |
| author_facet | Zhu Yuan-Mao Yu Zuo Cai Hong Gao Yu-Ran Dong Xiu-Mei Li Zhao-Li Shi Hong-Fei Meng Qing-Feng Lu Chuang Xue Fei |
| author_sort | Zhu Yuan-Mao |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Bovine adenovirus type 3 (BAV-3) belongs to the <it>Mastadenovirus </it>genus of the family <it>Adenoviridae </it>and is involved in respiratory and enteric infections of calves. The isolation of BAV-3 has not been reported prior to this study in China. In 2009, there were many cases in cattle showing similar clinical signs to BAV-3 infection and a virus strain, showing cytopathic effect in Madin-Darby bovine kidney cells, was isolated from a bovine nasal swab collected from feedlot cattle in Heilongjiang Province, China. The isolate was confirmed as a bovine adenovirus type 3 by PCR and immunofluorescence assay, and named as HLJ0955. So far only the complete genome sequence of prototype of BAV-3 WBR-1 strain has been reported. In order to further characterize the Chinese isolate HLJ0955, the complete genome sequence of HLJ0955 was determined.</p> <p>Results</p> <p>The size of the genome of the Chinese isolate HLJ0955 is 34,132 nucleotides in length with a G+C content of 53.6%. The coding sequences for gene regions of HLJ0955 isolate were similar to the prototype of BAV-3 WBR-1 strain, with 80.0-98.6% nucleotide and 87.5-98.8% amino acid identities. The genome of HLJ0955 strain contains 16 regions and four deletions in inverted terminal repeats, E1B region and E4 region, respectively. The complete genome and DNA binding protein gene based phylogenetic analysis with other adenoviruses were performed and the results showed that HLJ0955 isolate belonged to BAV-3 and clustered within the <it>Mastadenovirus </it>genus of the family <it>Adenoviridae</it>.</p> <p>Conclusions</p> <p>This is the first study to report the isolation and molecular characterization of BAV-3 from cattle in China. The phylogenetic analysis performed in this study supported the use of the DNA binding protein gene of adenovirus as an appropriate subgenomic target for the classification of different genuses of the family <it>Adenoviridae </it>on the molecular basis. Meanwhile, a large-scale pathogen and serological epidemiological investigations for BVA-3 infection might be carried out in cattle in China. This report will be a good beginning for further studies on BAV-3 in China.</p> |
| first_indexed | 2024-12-12T09:02:26Z |
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| spelling | doaj.art-0d29f21959994091bd15845275c1f69e2022-12-22T00:29:47ZengBMCVirology Journal1743-422X2011-12-018155710.1186/1743-422X-8-557Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in ChinaZhu Yuan-MaoYu ZuoCai HongGao Yu-RanDong Xiu-MeiLi Zhao-LiShi Hong-FeiMeng Qing-FengLu ChuangXue Fei<p>Abstract</p> <p>Background</p> <p>Bovine adenovirus type 3 (BAV-3) belongs to the <it>Mastadenovirus </it>genus of the family <it>Adenoviridae </it>and is involved in respiratory and enteric infections of calves. The isolation of BAV-3 has not been reported prior to this study in China. In 2009, there were many cases in cattle showing similar clinical signs to BAV-3 infection and a virus strain, showing cytopathic effect in Madin-Darby bovine kidney cells, was isolated from a bovine nasal swab collected from feedlot cattle in Heilongjiang Province, China. The isolate was confirmed as a bovine adenovirus type 3 by PCR and immunofluorescence assay, and named as HLJ0955. So far only the complete genome sequence of prototype of BAV-3 WBR-1 strain has been reported. In order to further characterize the Chinese isolate HLJ0955, the complete genome sequence of HLJ0955 was determined.</p> <p>Results</p> <p>The size of the genome of the Chinese isolate HLJ0955 is 34,132 nucleotides in length with a G+C content of 53.6%. The coding sequences for gene regions of HLJ0955 isolate were similar to the prototype of BAV-3 WBR-1 strain, with 80.0-98.6% nucleotide and 87.5-98.8% amino acid identities. The genome of HLJ0955 strain contains 16 regions and four deletions in inverted terminal repeats, E1B region and E4 region, respectively. The complete genome and DNA binding protein gene based phylogenetic analysis with other adenoviruses were performed and the results showed that HLJ0955 isolate belonged to BAV-3 and clustered within the <it>Mastadenovirus </it>genus of the family <it>Adenoviridae</it>.</p> <p>Conclusions</p> <p>This is the first study to report the isolation and molecular characterization of BAV-3 from cattle in China. The phylogenetic analysis performed in this study supported the use of the DNA binding protein gene of adenovirus as an appropriate subgenomic target for the classification of different genuses of the family <it>Adenoviridae </it>on the molecular basis. Meanwhile, a large-scale pathogen and serological epidemiological investigations for BVA-3 infection might be carried out in cattle in China. This report will be a good beginning for further studies on BAV-3 in China.</p>http://www.virologyj.com/content/8/1/557Bovine adenovirus type 3CattleComplete genomeDNA binding protein |
| spellingShingle | Zhu Yuan-Mao Yu Zuo Cai Hong Gao Yu-Ran Dong Xiu-Mei Li Zhao-Li Shi Hong-Fei Meng Qing-Feng Lu Chuang Xue Fei Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China Virology Journal Bovine adenovirus type 3 Cattle Complete genome DNA binding protein |
| title | Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China |
| title_full | Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China |
| title_fullStr | Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China |
| title_full_unstemmed | Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China |
| title_short | Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China |
| title_sort | isolation identification and complete genome sequence of a bovine adenovirus type 3 from cattle in china |
| topic | Bovine adenovirus type 3 Cattle Complete genome DNA binding protein |
| url | http://www.virologyj.com/content/8/1/557 |
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