Specific Antibodies Reacting with SV40 Large T Antigen Mimotopes in Serum Samples of Healthy Subjects.
Simian Virus 40, experimentally assayed in vitro in different animal and human cells and in vivo in rodents, was classified as a small DNA tumor virus. In previous studies, many groups identified Simian Virus 40 sequences in healthy individuals and cancer patients using PCR techniques, whereas other...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4701414?pdf=render |
| _version_ | 1818432378246266880 |
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| author | Mauro Tognon Alfredo Corallini Marco Manfrini Angelo Taronna Janet S Butel Silvia Pietrobon Lorenzo Trevisiol Ilaria Bononi Emanuela Vaccher Giuseppe Barbanti-Brodano Fernanda Martini Elisa Mazzoni |
| author_facet | Mauro Tognon Alfredo Corallini Marco Manfrini Angelo Taronna Janet S Butel Silvia Pietrobon Lorenzo Trevisiol Ilaria Bononi Emanuela Vaccher Giuseppe Barbanti-Brodano Fernanda Martini Elisa Mazzoni |
| author_sort | Mauro Tognon |
| collection | DOAJ |
| description | Simian Virus 40, experimentally assayed in vitro in different animal and human cells and in vivo in rodents, was classified as a small DNA tumor virus. In previous studies, many groups identified Simian Virus 40 sequences in healthy individuals and cancer patients using PCR techniques, whereas others failed to detect the viral sequences in human specimens. These conflicting results prompted us to develop a novel indirect ELISA with synthetic peptides, mimicking Simian Virus 40 capsid viral protein antigens, named mimotopes. This immunologic assay allowed us to investigate the presence of serum antibodies against Simian Virus 40 and to verify whether Simian Virus 40 is circulating in humans. In this investigation two mimotopes from Simian Virus 40 large T antigen, the viral replication protein and oncoprotein, were employed to analyze for specific reactions to human sera antibodies. This indirect ELISA with synthetic peptides from Simian Virus 40 large T antigen was used to assay a new collection of serum samples from healthy subjects. This novel assay revealed that serum antibodies against Simian Virus 40 large T antigen mimotopes are detectable, at low titer, in healthy subjects aged from 18-65 years old. The overall prevalence of reactivity with the two Simian Virus 40 large T antigen peptides was 20%. This new ELISA with two mimotopes of the early viral regions is able to detect in a specific manner Simian Virus 40 large T antigen-antibody responses. |
| first_indexed | 2024-12-14T16:04:15Z |
| format | Article |
| id | doaj.art-0d2c2827a1584231b581a5224413c622 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T16:04:15Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0d2c2827a1584231b581a5224413c6222022-12-21T22:55:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01111e014572010.1371/journal.pone.0145720Specific Antibodies Reacting with SV40 Large T Antigen Mimotopes in Serum Samples of Healthy Subjects.Mauro TognonAlfredo CoralliniMarco ManfriniAngelo TaronnaJanet S ButelSilvia PietrobonLorenzo TrevisiolIlaria BononiEmanuela VaccherGiuseppe Barbanti-BrodanoFernanda MartiniElisa MazzoniSimian Virus 40, experimentally assayed in vitro in different animal and human cells and in vivo in rodents, was classified as a small DNA tumor virus. In previous studies, many groups identified Simian Virus 40 sequences in healthy individuals and cancer patients using PCR techniques, whereas others failed to detect the viral sequences in human specimens. These conflicting results prompted us to develop a novel indirect ELISA with synthetic peptides, mimicking Simian Virus 40 capsid viral protein antigens, named mimotopes. This immunologic assay allowed us to investigate the presence of serum antibodies against Simian Virus 40 and to verify whether Simian Virus 40 is circulating in humans. In this investigation two mimotopes from Simian Virus 40 large T antigen, the viral replication protein and oncoprotein, were employed to analyze for specific reactions to human sera antibodies. This indirect ELISA with synthetic peptides from Simian Virus 40 large T antigen was used to assay a new collection of serum samples from healthy subjects. This novel assay revealed that serum antibodies against Simian Virus 40 large T antigen mimotopes are detectable, at low titer, in healthy subjects aged from 18-65 years old. The overall prevalence of reactivity with the two Simian Virus 40 large T antigen peptides was 20%. This new ELISA with two mimotopes of the early viral regions is able to detect in a specific manner Simian Virus 40 large T antigen-antibody responses.http://europepmc.org/articles/PMC4701414?pdf=render |
| spellingShingle | Mauro Tognon Alfredo Corallini Marco Manfrini Angelo Taronna Janet S Butel Silvia Pietrobon Lorenzo Trevisiol Ilaria Bononi Emanuela Vaccher Giuseppe Barbanti-Brodano Fernanda Martini Elisa Mazzoni Specific Antibodies Reacting with SV40 Large T Antigen Mimotopes in Serum Samples of Healthy Subjects. PLoS ONE |
| title | Specific Antibodies Reacting with SV40 Large T Antigen Mimotopes in Serum Samples of Healthy Subjects. |
| title_full | Specific Antibodies Reacting with SV40 Large T Antigen Mimotopes in Serum Samples of Healthy Subjects. |
| title_fullStr | Specific Antibodies Reacting with SV40 Large T Antigen Mimotopes in Serum Samples of Healthy Subjects. |
| title_full_unstemmed | Specific Antibodies Reacting with SV40 Large T Antigen Mimotopes in Serum Samples of Healthy Subjects. |
| title_short | Specific Antibodies Reacting with SV40 Large T Antigen Mimotopes in Serum Samples of Healthy Subjects. |
| title_sort | specific antibodies reacting with sv40 large t antigen mimotopes in serum samples of healthy subjects |
| url | http://europepmc.org/articles/PMC4701414?pdf=render |
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