Immune and Genomic Analysis of Boxer Dog Breed and Its Relationship with <i>Leishmania infantum</i> Infection
Leishmaniosis, one of the most important zoonoses in Europe, is caused <i>by Leishmania infantum</i>, an intracellular protozoan parasite. This disease is endemic in the Mediterranean area, where the main reservoir is the dog. Several studies indicate a possible susceptibility to <i&g...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/9/11/608 |
| _version_ | 1827645330370330624 |
|---|---|
| author | Luis Álvarez Pablo-Jesús Marín-García Pilar Rentero-Garrido Lola Llobat |
| author_facet | Luis Álvarez Pablo-Jesús Marín-García Pilar Rentero-Garrido Lola Llobat |
| author_sort | Luis Álvarez |
| collection | DOAJ |
| description | Leishmaniosis, one of the most important zoonoses in Europe, is caused <i>by Leishmania infantum</i>, an intracellular protozoan parasite. This disease is endemic in the Mediterranean area, where the main reservoir is the dog. Several studies indicate a possible susceptibility to <i>L. infantum</i> infection with clinical signs in some canine breeds. One of them is the boxer breed, which shows a high prevalence of disease. In this study, immunological and genomic characterization of serum samples from boxer dogs living in the Mediterranean area were evaluated to analyze the immune response and the possible genetic explanation for this susceptibility. Serum levels of cytokines IFN-γ, IL-2, IL-6, IL-8, and IL-18 were determined by ELISA commercial tests, while the genotyping study was performed using the CanineHD DNA Analysis BeadChip. The results show relevant differences in the serum levels of cytokines compared to published data on other canine breeds, as well as sequence changes that could explain the high susceptibility of the boxer breed to the disease. Concretely, polymorphic variants in the <i>CIITA</i>, <i>HSF2BP</i>, <i>LTBP1</i>, <i>MITF</i>, <i>NOXA1</i>, <i>PKIB</i>, <i>RAB38</i>, <i>RASEF</i>, <i>TLE1</i>, and <i>TLR4</i> genes were found, which could explain the susceptibility of this breed to <i>L. infantum</i> infection. |
| first_indexed | 2024-03-09T18:33:50Z |
| format | Article |
| id | doaj.art-0d2c8fe628964300ae133f5b0c9dd4af |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T18:33:50Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-0d2c8fe628964300ae133f5b0c9dd4af2023-11-24T07:14:57ZengMDPI AGVeterinary Sciences2306-73812022-11-0191160810.3390/vetsci9110608Immune and Genomic Analysis of Boxer Dog Breed and Its Relationship with <i>Leishmania infantum</i> InfectionLuis Álvarez0Pablo-Jesús Marín-García1Pilar Rentero-Garrido2Lola Llobat3Departamento Producción y Sanidad Animal, Salud Pública y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46010 Valencia, SpainDepartamento Producción y Sanidad Animal, Salud Pública y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46010 Valencia, SpainDepartamento Producción y Sanidad Animal, Salud Pública y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46010 Valencia, SpainDepartamento Producción y Sanidad Animal, Salud Pública y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46010 Valencia, SpainLeishmaniosis, one of the most important zoonoses in Europe, is caused <i>by Leishmania infantum</i>, an intracellular protozoan parasite. This disease is endemic in the Mediterranean area, where the main reservoir is the dog. Several studies indicate a possible susceptibility to <i>L. infantum</i> infection with clinical signs in some canine breeds. One of them is the boxer breed, which shows a high prevalence of disease. In this study, immunological and genomic characterization of serum samples from boxer dogs living in the Mediterranean area were evaluated to analyze the immune response and the possible genetic explanation for this susceptibility. Serum levels of cytokines IFN-γ, IL-2, IL-6, IL-8, and IL-18 were determined by ELISA commercial tests, while the genotyping study was performed using the CanineHD DNA Analysis BeadChip. The results show relevant differences in the serum levels of cytokines compared to published data on other canine breeds, as well as sequence changes that could explain the high susceptibility of the boxer breed to the disease. Concretely, polymorphic variants in the <i>CIITA</i>, <i>HSF2BP</i>, <i>LTBP1</i>, <i>MITF</i>, <i>NOXA1</i>, <i>PKIB</i>, <i>RAB38</i>, <i>RASEF</i>, <i>TLE1</i>, and <i>TLR4</i> genes were found, which could explain the susceptibility of this breed to <i>L. infantum</i> infection.https://www.mdpi.com/2306-7381/9/11/608canine breedleishmaniosisboxer dog<i>Leishmania</i>Mediterranean region |
| spellingShingle | Luis Álvarez Pablo-Jesús Marín-García Pilar Rentero-Garrido Lola Llobat Immune and Genomic Analysis of Boxer Dog Breed and Its Relationship with <i>Leishmania infantum</i> Infection Veterinary Sciences canine breed leishmaniosis boxer dog <i>Leishmania</i> Mediterranean region |
| title | Immune and Genomic Analysis of Boxer Dog Breed and Its Relationship with <i>Leishmania infantum</i> Infection |
| title_full | Immune and Genomic Analysis of Boxer Dog Breed and Its Relationship with <i>Leishmania infantum</i> Infection |
| title_fullStr | Immune and Genomic Analysis of Boxer Dog Breed and Its Relationship with <i>Leishmania infantum</i> Infection |
| title_full_unstemmed | Immune and Genomic Analysis of Boxer Dog Breed and Its Relationship with <i>Leishmania infantum</i> Infection |
| title_short | Immune and Genomic Analysis of Boxer Dog Breed and Its Relationship with <i>Leishmania infantum</i> Infection |
| title_sort | immune and genomic analysis of boxer dog breed and its relationship with i leishmania infantum i infection |
| topic | canine breed leishmaniosis boxer dog <i>Leishmania</i> Mediterranean region |
| url | https://www.mdpi.com/2306-7381/9/11/608 |
| work_keys_str_mv | AT luisalvarez immuneandgenomicanalysisofboxerdogbreedanditsrelationshipwithileishmaniainfantumiinfection AT pablojesusmaringarcia immuneandgenomicanalysisofboxerdogbreedanditsrelationshipwithileishmaniainfantumiinfection AT pilarrenterogarrido immuneandgenomicanalysisofboxerdogbreedanditsrelationshipwithileishmaniainfantumiinfection AT lolallobat immuneandgenomicanalysisofboxerdogbreedanditsrelationshipwithileishmaniainfantumiinfection |