An evaluation of the microbiota of the upper reproductive tract of women with and without epithelial ovarian cancer
Objective: The microbiome of the female upper reproductive tract (URT) has not been characterized. We hypothesize that distinct bacterial species may be identified in different areas of the URT in women with or without ovarian cancers. Methods: Postmenopausal women scheduled for salpingooophorectomy...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Gynecologic Oncology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352578922000972 |
| _version_ | 1818015045053841408 |
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| author | Wendy R. Brewster Wesley C. Burkett Emily M. Ko Victoria Bae-Jump Amber Nicole McCoy Temitope O. Keku |
| author_facet | Wendy R. Brewster Wesley C. Burkett Emily M. Ko Victoria Bae-Jump Amber Nicole McCoy Temitope O. Keku |
| author_sort | Wendy R. Brewster |
| collection | DOAJ |
| description | Objective: The microbiome of the female upper reproductive tract (URT) has not been characterized. We hypothesize that distinct bacterial species may be identified in different areas of the URT in women with or without ovarian cancers. Methods: Postmenopausal women scheduled for salpingooophorectomy were prospectively identified. We excluded those who used antibiotics within three months of surgery or had a diagnosed gynecologic cancer. Bacteria were extracted from tissue samples of the proximal fallopian tube, fimbriae and ovaries of 10 women. Using molecular-phylogenetic methods based on the highly conserved 16S bacteria rRNA gene, we assessed the complexity of URT microbiota in tissue samples by high throughput sequencing of the V1-V3 region of the 16S gene. Sequences were processed through QIIME and an average of 69,625 reads per sample was obtained after quality filtering. Multivariate analyses were conducted using PRIMER VI software. Results: The initial analysis of samples suggests that bacteria exist in the URT. Analysis of similarity matrix (ANOSIM) suggests that the microbiome differs in the areas examined (ANOSIM R = 0.26, p = 0.015). The microbiome differs significantly between the fallopian tube and ovary (ANOSIM R = 0.23, p = 0.02). The proximal fallopian tube microbiome also differs from the fimbriae (ANOSIM R = 0.66, p = 0.025). There were borderline differences in the microbial profiles of the specimens with and without epithelial ovarian cancer (p = 0.06). Conclusions: We identified distinct microbiota of the ovaries and fallopian tubes with a profile unique to women with epithelial ovarian cancer. Further investigation is necessary to determine whether the microbiome is related to ovarian carcinogenesis. |
| first_indexed | 2024-04-14T06:52:03Z |
| format | Article |
| id | doaj.art-0d2d90ee4c4a4231b11eca1bd07e3de1 |
| institution | Directory Open Access Journal |
| issn | 2352-5789 |
| language | English |
| last_indexed | 2024-04-14T06:52:03Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Gynecologic Oncology Reports |
| spelling | doaj.art-0d2d90ee4c4a4231b11eca1bd07e3de12022-12-22T02:06:59ZengElsevierGynecologic Oncology Reports2352-57892022-08-0142101017An evaluation of the microbiota of the upper reproductive tract of women with and without epithelial ovarian cancerWendy R. Brewster0Wesley C. Burkett1Emily M. Ko2Victoria Bae-Jump3Amber Nicole McCoy4Temitope O. Keku5University of North Carolina at Chapel Hill, Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, United States; University of North Carolina at Chapel Hill, Lineberger Comprehensive Cancer Center, United States; Corresponding author at: University of North Carolina at Chapel Hill, Physician’s Office Building B103, 170 Manning Drive CB 7572, Chapel Hill, NC 27599, United StatesUniversity of North Carolina at Chapel Hill, Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, United StatesUniversity of Pennsylvania Health Systems, Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, United StatesUniversity of North Carolina at Chapel Hill, Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, United States; University of North Carolina at Chapel Hill, Lineberger Comprehensive Cancer Center, United StatesUniversity of North Carolina at Chapel Hill, Department of Medicine, Center for Gastrointestinal Biology and Disease, United StatesUniversity of North Carolina at Chapel Hill, Department of Medicine, Center for Gastrointestinal Biology and Disease, United StatesObjective: The microbiome of the female upper reproductive tract (URT) has not been characterized. We hypothesize that distinct bacterial species may be identified in different areas of the URT in women with or without ovarian cancers. Methods: Postmenopausal women scheduled for salpingooophorectomy were prospectively identified. We excluded those who used antibiotics within three months of surgery or had a diagnosed gynecologic cancer. Bacteria were extracted from tissue samples of the proximal fallopian tube, fimbriae and ovaries of 10 women. Using molecular-phylogenetic methods based on the highly conserved 16S bacteria rRNA gene, we assessed the complexity of URT microbiota in tissue samples by high throughput sequencing of the V1-V3 region of the 16S gene. Sequences were processed through QIIME and an average of 69,625 reads per sample was obtained after quality filtering. Multivariate analyses were conducted using PRIMER VI software. Results: The initial analysis of samples suggests that bacteria exist in the URT. Analysis of similarity matrix (ANOSIM) suggests that the microbiome differs in the areas examined (ANOSIM R = 0.26, p = 0.015). The microbiome differs significantly between the fallopian tube and ovary (ANOSIM R = 0.23, p = 0.02). The proximal fallopian tube microbiome also differs from the fimbriae (ANOSIM R = 0.66, p = 0.025). There were borderline differences in the microbial profiles of the specimens with and without epithelial ovarian cancer (p = 0.06). Conclusions: We identified distinct microbiota of the ovaries and fallopian tubes with a profile unique to women with epithelial ovarian cancer. Further investigation is necessary to determine whether the microbiome is related to ovarian carcinogenesis.http://www.sciencedirect.com/science/article/pii/S2352578922000972Ovary microbiomeFallopian tube microbiomeUpper reproductive tract microbiomeOvarian cancer |
| spellingShingle | Wendy R. Brewster Wesley C. Burkett Emily M. Ko Victoria Bae-Jump Amber Nicole McCoy Temitope O. Keku An evaluation of the microbiota of the upper reproductive tract of women with and without epithelial ovarian cancer Gynecologic Oncology Reports Ovary microbiome Fallopian tube microbiome Upper reproductive tract microbiome Ovarian cancer |
| title | An evaluation of the microbiota of the upper reproductive tract of women with and without epithelial ovarian cancer |
| title_full | An evaluation of the microbiota of the upper reproductive tract of women with and without epithelial ovarian cancer |
| title_fullStr | An evaluation of the microbiota of the upper reproductive tract of women with and without epithelial ovarian cancer |
| title_full_unstemmed | An evaluation of the microbiota of the upper reproductive tract of women with and without epithelial ovarian cancer |
| title_short | An evaluation of the microbiota of the upper reproductive tract of women with and without epithelial ovarian cancer |
| title_sort | evaluation of the microbiota of the upper reproductive tract of women with and without epithelial ovarian cancer |
| topic | Ovary microbiome Fallopian tube microbiome Upper reproductive tract microbiome Ovarian cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2352578922000972 |
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