Prognostic value of neutrophil extracellular trap signature in clear cell renal cell carcinoma
IntroductionClear cell renal cell carcinoma (ccRCC) is the most prevalent type of urological carcinoma. Although targeted therapy and immunotherapy are usually employed, they often result in primary and acquired resistance. There is currently a lack of dependable biomarkers that can accurately antic...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1205713/full |
| _version_ | 1797781689079955456 |
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| author | Rong Li Xuewen Jiang Pin Wang Xiaoyan Liu |
| author_facet | Rong Li Xuewen Jiang Pin Wang Xiaoyan Liu |
| author_sort | Rong Li |
| collection | DOAJ |
| description | IntroductionClear cell renal cell carcinoma (ccRCC) is the most prevalent type of urological carcinoma. Although targeted therapy and immunotherapy are usually employed, they often result in primary and acquired resistance. There is currently a lack of dependable biomarkers that can accurately anticipate the prognosis of ccRCC. Recent research has indicated the critical role of neutrophil extracellular traps (NETs) in the development, metastasis, and immune evasion of cancer. The aim of this study was to explore the value of NETs in the development and prognosis of ccRCC.MethodsClinical features and genetic expression information of ccRCC patients were acquired from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC) and E-MTAB-1980 database. NETs-related gene set were obtained from previous studies. A NETs-related gene signature was constructed based on TCGA data and validated using ICGC and E-MTAB-1980 databases. Furthermore, the immune microenvironment and responsiveness to anticancer medications in ccRCC patients with varying levels of NETs risks were investigated.ResultsA total of 31 NET-related genes were differently expressed between normal kidney and ccRCC tissues. 17 out of 31 were significantly associated with overall survival. After LASSO Cox regression analysis, nine NETs-related genes were enrolled to construct the NETs prognosis signature, and all the ccRCC patients from TCGA were divided into low and high risk group. This signature demonstrated excellent performance in predicting the overall survival of TCGA patients as well as the validation ICGC and E-MTAB-1980 patients. Additionally, the NETs signature was significantly correlated with immune infiltration and drug sensitivity.ConclusionsThe NETs signature established by the current study has prognostic significance in ccRCC, and may serve as a useful biomarker for patient stratification and treatment decisions. Further validation and clinical studies are required to fully translate these findings into clinical practice. |
| first_indexed | 2024-03-13T00:00:36Z |
| format | Article |
| id | doaj.art-0d2e0475c20d4753ab64f05cf91fb0e1 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-03-13T00:00:36Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-0d2e0475c20d4753ab64f05cf91fb0e12023-07-13T10:53:39ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-07-011310.3389/fonc.2023.12057131205713Prognostic value of neutrophil extracellular trap signature in clear cell renal cell carcinomaRong Li0Xuewen Jiang1Pin Wang2Xiaoyan Liu3Phase I Clinical Trial Center, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Urology, Qilu Hospital of Shandong University, Jinan, ChinaNational Health Commission (NHC) Key Laboratory of Otorhinolaryngology, Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Shandong University, Jinan, Shandong, ChinaPhase I Clinical Trial Center, Qilu Hospital of Shandong University, Jinan, ChinaIntroductionClear cell renal cell carcinoma (ccRCC) is the most prevalent type of urological carcinoma. Although targeted therapy and immunotherapy are usually employed, they often result in primary and acquired resistance. There is currently a lack of dependable biomarkers that can accurately anticipate the prognosis of ccRCC. Recent research has indicated the critical role of neutrophil extracellular traps (NETs) in the development, metastasis, and immune evasion of cancer. The aim of this study was to explore the value of NETs in the development and prognosis of ccRCC.MethodsClinical features and genetic expression information of ccRCC patients were acquired from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC) and E-MTAB-1980 database. NETs-related gene set were obtained from previous studies. A NETs-related gene signature was constructed based on TCGA data and validated using ICGC and E-MTAB-1980 databases. Furthermore, the immune microenvironment and responsiveness to anticancer medications in ccRCC patients with varying levels of NETs risks were investigated.ResultsA total of 31 NET-related genes were differently expressed between normal kidney and ccRCC tissues. 17 out of 31 were significantly associated with overall survival. After LASSO Cox regression analysis, nine NETs-related genes were enrolled to construct the NETs prognosis signature, and all the ccRCC patients from TCGA were divided into low and high risk group. This signature demonstrated excellent performance in predicting the overall survival of TCGA patients as well as the validation ICGC and E-MTAB-1980 patients. Additionally, the NETs signature was significantly correlated with immune infiltration and drug sensitivity.ConclusionsThe NETs signature established by the current study has prognostic significance in ccRCC, and may serve as a useful biomarker for patient stratification and treatment decisions. Further validation and clinical studies are required to fully translate these findings into clinical practice.https://www.frontiersin.org/articles/10.3389/fonc.2023.1205713/fullclear cell renal cell carcinomaneutrophil extracellular trapsprognosis signaturevalidationmultiomics dataimmune microenvironment |
| spellingShingle | Rong Li Xuewen Jiang Pin Wang Xiaoyan Liu Prognostic value of neutrophil extracellular trap signature in clear cell renal cell carcinoma Frontiers in Oncology clear cell renal cell carcinoma neutrophil extracellular traps prognosis signature validation multiomics data immune microenvironment |
| title | Prognostic value of neutrophil extracellular trap signature in clear cell renal cell carcinoma |
| title_full | Prognostic value of neutrophil extracellular trap signature in clear cell renal cell carcinoma |
| title_fullStr | Prognostic value of neutrophil extracellular trap signature in clear cell renal cell carcinoma |
| title_full_unstemmed | Prognostic value of neutrophil extracellular trap signature in clear cell renal cell carcinoma |
| title_short | Prognostic value of neutrophil extracellular trap signature in clear cell renal cell carcinoma |
| title_sort | prognostic value of neutrophil extracellular trap signature in clear cell renal cell carcinoma |
| topic | clear cell renal cell carcinoma neutrophil extracellular traps prognosis signature validation multiomics data immune microenvironment |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1205713/full |
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