Protective activity of hydroalcoholic extract of zingiber officinale rosc. on doxorubicin-induced hepatic toxicity in male rats
Marzieh Niakan1 , Habib Alla Johari 2, Mehrdad Shariati1 , Davood Moghadamnia3 , Ebrahim Talebi4 Background: Research has shown that the extract of some plants has an effective protective effect on liver cells against toxins and oxidants. In this research, the effect of hydroalcoholic extract...
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2023-07-01
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| Series: | Medical Laboratory Journal |
| Subjects: | |
| Online Access: | http://mlj.goums.ac.ir/article-1-1542-en.pdf |
| _version_ | 1797301235592724480 |
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| author | Marzieh Niakan Habib Alla Johari Mehrdad Shariati Davood Moghadamnia Ebrahim Talebi |
| author_facet | Marzieh Niakan Habib Alla Johari Mehrdad Shariati Davood Moghadamnia Ebrahim Talebi |
| author_sort | Marzieh Niakan |
| collection | DOAJ |
| description | Marzieh Niakan1 , Habib Alla Johari 2, Mehrdad Shariati1 , Davood Moghadamnia3 , Ebrahim Talebi4
Background: Research has shown that the extract of some plants has an effective protective effect on liver cells against toxins and oxidants. In this research, the effect of hydroalcoholic extract of ginger against hepatotoxicity caused by doxorubicin (Dox) in adult male rats was investigated.
Methods: In this experimental study, 63 adult male rats were divided into 7 groups (n = 9 per group). The experimental treatments included the control and placebo groups without the use of medicines, experimental group I that received Dox at a dose of 20 mg/kg, experimental groups II and III with 300 and 600 mg/kg of ginger hydroalcoholic extract, and experimental groups IV and V that received 20 mg/kg Dox + 300 and 600 mg/kg of ginger hydroalcoholic extract, respectively. After the end of the test period, serum levels of total bilirubin and direct bilirubin were measured. In addition, the histological changes in the liver were examined after hematoxylin and eosin (H&E) staining.
Results: Serum levels of total bilirubin and direct bilirubin in the Dox group showed a significant increase compared to the control group. In contrast, serum levels of total bilirubin and direct bilirubin in Dox + ZIN 600 and Dox + ZIN 300 had a significant reduction compared to the Dox group (P < 0.05). Ginger extract prevented apoptosis and Dox-induced liver tissue damage in dose-dependent designs.
Conclusion: The hydroalcoholic extract of ginger improves the changes of serum bilirubin and liver tissue after receiving Dox due to its antioxidant compounds. |
| first_indexed | 2024-03-07T23:19:35Z |
| format | Article |
| id | doaj.art-0d30d6a1ea05430885155f8b6de523e1 |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-03-07T23:19:35Z |
| publishDate | 2023-07-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-0d30d6a1ea05430885155f8b6de523e12024-02-21T07:03:15ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492023-07-01174912Protective activity of hydroalcoholic extract of zingiber officinale rosc. on doxorubicin-induced hepatic toxicity in male ratsMarzieh Niakan0Habib Alla Johari1Mehrdad Shariati2Davood Moghadamnia3Ebrahim Talebi4 Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran Department of Biology, Darab Branch, Islamic Azad University, Darab, Iran Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz,Iran Department of Animal Sciences, Darab Branch, Islamic Azad University, Darab, Fars, Iran Marzieh Niakan1 , Habib Alla Johari 2, Mehrdad Shariati1 , Davood Moghadamnia3 , Ebrahim Talebi4 Background: Research has shown that the extract of some plants has an effective protective effect on liver cells against toxins and oxidants. In this research, the effect of hydroalcoholic extract of ginger against hepatotoxicity caused by doxorubicin (Dox) in adult male rats was investigated. Methods: In this experimental study, 63 adult male rats were divided into 7 groups (n = 9 per group). The experimental treatments included the control and placebo groups without the use of medicines, experimental group I that received Dox at a dose of 20 mg/kg, experimental groups II and III with 300 and 600 mg/kg of ginger hydroalcoholic extract, and experimental groups IV and V that received 20 mg/kg Dox + 300 and 600 mg/kg of ginger hydroalcoholic extract, respectively. After the end of the test period, serum levels of total bilirubin and direct bilirubin were measured. In addition, the histological changes in the liver were examined after hematoxylin and eosin (H&E) staining. Results: Serum levels of total bilirubin and direct bilirubin in the Dox group showed a significant increase compared to the control group. In contrast, serum levels of total bilirubin and direct bilirubin in Dox + ZIN 600 and Dox + ZIN 300 had a significant reduction compared to the Dox group (P < 0.05). Ginger extract prevented apoptosis and Dox-induced liver tissue damage in dose-dependent designs. Conclusion: The hydroalcoholic extract of ginger improves the changes of serum bilirubin and liver tissue after receiving Dox due to its antioxidant compounds.http://mlj.goums.ac.ir/article-1-1542-en.pdfginger extractdoxorubicinadverse effectsliver failurerat |
| spellingShingle | Marzieh Niakan Habib Alla Johari Mehrdad Shariati Davood Moghadamnia Ebrahim Talebi Protective activity of hydroalcoholic extract of zingiber officinale rosc. on doxorubicin-induced hepatic toxicity in male rats Medical Laboratory Journal ginger extract doxorubicin adverse effects liver failure rat |
| title | Protective activity of hydroalcoholic extract of zingiber officinale rosc. on doxorubicin-induced hepatic toxicity in male rats |
| title_full | Protective activity of hydroalcoholic extract of zingiber officinale rosc. on doxorubicin-induced hepatic toxicity in male rats |
| title_fullStr | Protective activity of hydroalcoholic extract of zingiber officinale rosc. on doxorubicin-induced hepatic toxicity in male rats |
| title_full_unstemmed | Protective activity of hydroalcoholic extract of zingiber officinale rosc. on doxorubicin-induced hepatic toxicity in male rats |
| title_short | Protective activity of hydroalcoholic extract of zingiber officinale rosc. on doxorubicin-induced hepatic toxicity in male rats |
| title_sort | protective activity of hydroalcoholic extract of zingiber officinale rosc on doxorubicin induced hepatic toxicity in male rats |
| topic | ginger extract doxorubicin adverse effects liver failure rat |
| url | http://mlj.goums.ac.ir/article-1-1542-en.pdf |
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