Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological features
ABSTRACTINTRODUCTION:The aim of this study was quantify annexin A1 expression in macrophages and cluster of differentiation 4 (CD4) + and cluster of differentiation 8 (CD8)+ T cells from the skin of patients with cutaneous leishmaniasis (n=55) and correlate with histopathological aspects.METHODS:Inf...
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Sociedade Brasileira de Medicina Tropical (SBMT)
2015-10-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500560&lng=en&tlng=en |
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| author | Helen Aguiar Lemes da Silva Gabriel Silva de Lima Mariana Côrtes Boité Renato Porrozzi Marcia Hueb Amilcar Sabino Damazo |
| author_facet | Helen Aguiar Lemes da Silva Gabriel Silva de Lima Mariana Côrtes Boité Renato Porrozzi Marcia Hueb Amilcar Sabino Damazo |
| author_sort | Helen Aguiar Lemes da Silva |
| collection | DOAJ |
| description | ABSTRACTINTRODUCTION:The aim of this study was quantify annexin A1 expression in macrophages and cluster of differentiation 4 (CD4) + and cluster of differentiation 8 (CD8)+ T cells from the skin of patients with cutaneous leishmaniasis (n=55) and correlate with histopathological aspects.METHODS:Infecting species were identified by polymerase chain reaction-restriction fragment length polymorphism, and expression of annexin A1 was analyzed by immunofluorescence.RESULTS:All patients (n = 55) were infected with Leishmania braziliensis . Annexin A1 was expressed more abundantly in CD163 + macrophages in infected skin (p < 0.0001) than in uninfected skin. In addition, macrophages in necrotic exudative reaction lesions expressed annexin A1 at higher levels than those observed in granulomatous (p < 0.01) and cellular lesions p < 0.05). This difference might be due to the need to clear both parasites and necrotic tissue from necrotic lesions. CD4 + cells in cellular lesions expressed annexin A1 more abundantly than did those in necrotic (p < 0.05) and granulomatous lesions (p < 0.01). Expression in CD8 + T cells followed the same trend. These differences might be due to the pervasiveness of lymphohistiocytic and plasmacytic infiltrate in cellular lesions.CONCLUSIONS:Annexin A1 is differentially expressed in CD163 + macrophages and T cells depending on the histopathological features of Leishmania -infected skin, which might affect cell activation. |
| first_indexed | 2024-04-12T11:04:06Z |
| format | Article |
| id | doaj.art-0d32e545413a4ce998cb762d02dbf723 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-04-12T11:04:06Z |
| publishDate | 2015-10-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-0d32e545413a4ce998cb762d02dbf7232022-12-22T03:35:50ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492015-10-0148556056710.1590/0037-8682-0183-2015S0037-86822015000500560Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological featuresHelen Aguiar Lemes da SilvaGabriel Silva de LimaMariana Côrtes BoitéRenato PorrozziMarcia HuebAmilcar Sabino DamazoABSTRACTINTRODUCTION:The aim of this study was quantify annexin A1 expression in macrophages and cluster of differentiation 4 (CD4) + and cluster of differentiation 8 (CD8)+ T cells from the skin of patients with cutaneous leishmaniasis (n=55) and correlate with histopathological aspects.METHODS:Infecting species were identified by polymerase chain reaction-restriction fragment length polymorphism, and expression of annexin A1 was analyzed by immunofluorescence.RESULTS:All patients (n = 55) were infected with Leishmania braziliensis . Annexin A1 was expressed more abundantly in CD163 + macrophages in infected skin (p < 0.0001) than in uninfected skin. In addition, macrophages in necrotic exudative reaction lesions expressed annexin A1 at higher levels than those observed in granulomatous (p < 0.01) and cellular lesions p < 0.05). This difference might be due to the need to clear both parasites and necrotic tissue from necrotic lesions. CD4 + cells in cellular lesions expressed annexin A1 more abundantly than did those in necrotic (p < 0.05) and granulomatous lesions (p < 0.01). Expression in CD8 + T cells followed the same trend. These differences might be due to the pervasiveness of lymphohistiocytic and plasmacytic infiltrate in cellular lesions.CONCLUSIONS:Annexin A1 is differentially expressed in CD163 + macrophages and T cells depending on the histopathological features of Leishmania -infected skin, which might affect cell activation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500560&lng=en&tlng=enCutaneous leishmaniasisAnnexin A1LymphocytesMacrophages |
| spellingShingle | Helen Aguiar Lemes da Silva Gabriel Silva de Lima Mariana Côrtes Boité Renato Porrozzi Marcia Hueb Amilcar Sabino Damazo Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological features Revista da Sociedade Brasileira de Medicina Tropical Cutaneous leishmaniasis Annexin A1 Lymphocytes Macrophages |
| title | Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological features |
| title_full | Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological features |
| title_fullStr | Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological features |
| title_full_unstemmed | Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological features |
| title_short | Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological features |
| title_sort | expression of annexin a1 in leishmania infected skin and its correlation with histopathological features |
| topic | Cutaneous leishmaniasis Annexin A1 Lymphocytes Macrophages |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500560&lng=en&tlng=en |
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