Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gut
Background and Objectives: The use of probiotics as an alternative to antibiotics in poultry diets has attracted a lot of interest recently. In this context, the determination of probiotic characteristics was evaluated on several isolates obtained from Iranian poultry’s gut. Materials and Methods:...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-04-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4097 |
| _version_ | 1797840319534858240 |
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| author | Somaye Makzum Parinaz Ghadam Mohaddaseh Ramezani |
| author_facet | Somaye Makzum Parinaz Ghadam Mohaddaseh Ramezani |
| author_sort | Somaye Makzum |
| collection | DOAJ |
| description |
Background and Objectives: The use of probiotics as an alternative to antibiotics in poultry diets has attracted a lot of interest recently. In this context, the determination of probiotic characteristics was evaluated on several isolates obtained from Iranian poultry’s gut.
Materials and Methods: Probiotic characteristics such as hemolysis activity, acid, bile, gastric juice tolerate, in vitro adhesion assay, cell surface properties (hydrophobicity, auto-aggregation and co-aggregation) and antibiotic susceptibility test were evaluated. Finally, selected isolates identified molecularly after temperature-salt tolerance and extracellular enzyme activity (amylase, protease and cellulose).
Results: Out of 362 strains isolated from native poultry in three geographical regions of Iran, nine strains (belong to Bacillus sp., Enterococcus sp., Pediococcus sp., Lactobacillus sp., Kluyveromyces sp.) showed resistance against gastrointestinal physiological conditions, desirable surface properties, ability to adhere to epithelial intestine cell line and antibiotic susceptibility. Also, these strains were discovered to be temperature-salt tolerant but, only a small number of them were able to produce hydrolase enzymes.
Conclusion: According to the results, the selected strains can be introduced as native probiotic candidates for utilization in novel poultry feeds.
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| first_indexed | 2024-04-09T16:13:01Z |
| format | Article |
| id | doaj.art-0d342788d0c54d70bd5a14b52c898255 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-09T16:13:01Z |
| publishDate | 2023-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-0d342788d0c54d70bd5a14b52c8982552023-04-24T06:54:23ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472023-04-01152Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gutSomaye Makzum0Parinaz Ghadam1Mohaddaseh Ramezani2Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, IranDepartment of Biotechnology, Faculty of Biological Sciences, Alzahra UniversityDepartment of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, IranMicroorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR, Tehran, Iran Background and Objectives: The use of probiotics as an alternative to antibiotics in poultry diets has attracted a lot of interest recently. In this context, the determination of probiotic characteristics was evaluated on several isolates obtained from Iranian poultry’s gut. Materials and Methods: Probiotic characteristics such as hemolysis activity, acid, bile, gastric juice tolerate, in vitro adhesion assay, cell surface properties (hydrophobicity, auto-aggregation and co-aggregation) and antibiotic susceptibility test were evaluated. Finally, selected isolates identified molecularly after temperature-salt tolerance and extracellular enzyme activity (amylase, protease and cellulose). Results: Out of 362 strains isolated from native poultry in three geographical regions of Iran, nine strains (belong to Bacillus sp., Enterococcus sp., Pediococcus sp., Lactobacillus sp., Kluyveromyces sp.) showed resistance against gastrointestinal physiological conditions, desirable surface properties, ability to adhere to epithelial intestine cell line and antibiotic susceptibility. Also, these strains were discovered to be temperature-salt tolerant but, only a small number of them were able to produce hydrolase enzymes. Conclusion: According to the results, the selected strains can be introduced as native probiotic candidates for utilization in novel poultry feeds. https://ijm.tums.ac.ir/index.php/ijm/article/view/4097Antibiotic;Bacillus sp;In vitro test;Poultry;Probiotic |
| spellingShingle | Somaye Makzum Parinaz Ghadam Mohaddaseh Ramezani Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gut Iranian Journal of Microbiology Antibiotic; Bacillus sp; In vitro test; Poultry; Probiotic |
| title | Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gut |
| title_full | Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gut |
| title_fullStr | Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gut |
| title_full_unstemmed | Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gut |
| title_short | Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gut |
| title_sort | isolation functional evaluation of probiotic properties and molecular identification of strains isolated from iranian poultry s gut |
| topic | Antibiotic; Bacillus sp; In vitro test; Poultry; Probiotic |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4097 |
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