Cloning, expression and characterization of an aspartate aminotransferase gene from Lactobacillus brevis CGMCC 1306

Cloning, expression and characterization of an aspartate aminotransferase gene from Lactobacillus brevis CGMCC 1306

An aspartate aminotransferase (AATase) gene from Lactobacillus brevis CGMCC 1306 was cloned, which contains a 1182-bp open reading frame coding for 393 amino acids (41.43 kDa). When expressed in Escherichia coli BL21 (DE3), the recombinant AATase was purified and subsequently characterized. The reco...

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Bibliographic Details
Main Authors: Sheng Hu, Xiang Zhang, Yi Lu, Yue-Cheng Lin, Dong-Fang Xie, Hui Fang, Jun Huang, Le-He Mei
Format: Article
Language:English
Published: Taylor & Francis Group 2017-05-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Aspartate aminotransferase
psychrophilic enzyme
phase diagram
phylogenetic analysis; Lactobacillus brevis
Online Access:http://dx.doi.org/10.1080/13102818.2017.1304181

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