Upregulated ENC1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancer
A better knowledge of the molecular process behind uterine corpus endometrial carcinoma (UCEC) is important for prognosis prediction and the development of innovative targeted gene therapies. The purpose of this research is to discover critical genes associated with UCEC. We analyzed the gene expres...
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| Format: | Article |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.919637/full |
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| author | Lingling He Lingling He Lingling He Wenjing He Ji Luo Ji Luo Ji Luo Minjuan Xu Minjuan Xu Minjuan Xu |
| author_facet | Lingling He Lingling He Lingling He Wenjing He Ji Luo Ji Luo Ji Luo Minjuan Xu Minjuan Xu Minjuan Xu |
| author_sort | Lingling He |
| collection | DOAJ |
| description | A better knowledge of the molecular process behind uterine corpus endometrial carcinoma (UCEC) is important for prognosis prediction and the development of innovative targeted gene therapies. The purpose of this research is to discover critical genes associated with UCEC. We analyzed the gene expression profiles of TCGA-UCEC and GSE17025, respectively, using Weighted Gene Co-expression Network Analysis (WGCNA) and differential gene expression analysis. From four sets of findings, a total of 95 overlapping genes were retrieved. On the 95 overlapping genes, KEGG pathway and GO enrichment analysis were conducted. Then, we mapped the PPI network of 95 overlapping genes using the STRING database. Twenty hub genes were evaluated using the Cytohubba plugin, including NR3C1, ATF3, KLF15, THRA, NR4A1, FOSB, PER3, HLF, NTRK3, EGR3, MAPK13, ARNTL2, PKM2, SCD, EIF5A, ADHFE1, RERGL, TUB, and ENC1. The expression levels of NR3C1, PKM2, and ENC1 were shown to be adversely linked with the survival time of UCEC patients using univariate Cox regression analysis and Kaplan-Meier survival calculation. ENC1 were also overexpressed in UCEC tumor tissues or cell lines, as shown by quantitative real-time PCR and Western blotting. Then we looked into it further and discovered that ENC1 expression was linked to tumor microenvironment and predicted various immunological checkpoints. In conclusion, our data indicate that ENC1 may be required for the development of UCEC and may serve as a future biomarker for diagnosis and therapy. |
| first_indexed | 2024-04-11T07:56:38Z |
| format | Article |
| id | doaj.art-0d3535e131174829aa98281d211503d6 |
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| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-11T07:56:38Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-0d3535e131174829aa98281d211503d62022-12-22T04:35:55ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-12-011010.3389/fcell.2022.919637919637Upregulated ENC1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancerLingling He0Lingling He1Lingling He2Wenjing He3Ji Luo4Ji Luo5Ji Luo6Minjuan Xu7Minjuan Xu8Minjuan Xu9Department of Obstetrics and Gynecology, Ganzhou People's Hospital, Ganzhou, ChinaDepartment of Obstetrics and Gynecology, Ganzhou Hospital-Nanfang Hospital, Southern Medical University, Ganzhou, ChinaDepartment of Obstetrics and Gynecology, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, ChinaDepartment of Endocrinology, Baoji Gaoxin Hospital, Baoji, ChinaDepartment of Obstetrics and Gynecology, Ganzhou People's Hospital, Ganzhou, ChinaDepartment of Obstetrics and Gynecology, Ganzhou Hospital-Nanfang Hospital, Southern Medical University, Ganzhou, ChinaDepartment of Obstetrics and Gynecology, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, ChinaDepartment of Obstetrics and Gynecology, Ganzhou People's Hospital, Ganzhou, ChinaDepartment of Obstetrics and Gynecology, Ganzhou Hospital-Nanfang Hospital, Southern Medical University, Ganzhou, ChinaDepartment of Obstetrics and Gynecology, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, ChinaA better knowledge of the molecular process behind uterine corpus endometrial carcinoma (UCEC) is important for prognosis prediction and the development of innovative targeted gene therapies. The purpose of this research is to discover critical genes associated with UCEC. We analyzed the gene expression profiles of TCGA-UCEC and GSE17025, respectively, using Weighted Gene Co-expression Network Analysis (WGCNA) and differential gene expression analysis. From four sets of findings, a total of 95 overlapping genes were retrieved. On the 95 overlapping genes, KEGG pathway and GO enrichment analysis were conducted. Then, we mapped the PPI network of 95 overlapping genes using the STRING database. Twenty hub genes were evaluated using the Cytohubba plugin, including NR3C1, ATF3, KLF15, THRA, NR4A1, FOSB, PER3, HLF, NTRK3, EGR3, MAPK13, ARNTL2, PKM2, SCD, EIF5A, ADHFE1, RERGL, TUB, and ENC1. The expression levels of NR3C1, PKM2, and ENC1 were shown to be adversely linked with the survival time of UCEC patients using univariate Cox regression analysis and Kaplan-Meier survival calculation. ENC1 were also overexpressed in UCEC tumor tissues or cell lines, as shown by quantitative real-time PCR and Western blotting. Then we looked into it further and discovered that ENC1 expression was linked to tumor microenvironment and predicted various immunological checkpoints. In conclusion, our data indicate that ENC1 may be required for the development of UCEC and may serve as a future biomarker for diagnosis and therapy.https://www.frontiersin.org/articles/10.3389/fcell.2022.919637/fullendometrial carcinomaENC1WGCNAprognosisimmunity therapy |
| spellingShingle | Lingling He Lingling He Lingling He Wenjing He Ji Luo Ji Luo Ji Luo Minjuan Xu Minjuan Xu Minjuan Xu Upregulated ENC1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancer Frontiers in Cell and Developmental Biology endometrial carcinoma ENC1 WGCNA prognosis immunity therapy |
| title | Upregulated ENC1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancer |
| title_full | Upregulated ENC1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancer |
| title_fullStr | Upregulated ENC1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancer |
| title_full_unstemmed | Upregulated ENC1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancer |
| title_short | Upregulated ENC1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancer |
| title_sort | upregulated enc1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancer |
| topic | endometrial carcinoma ENC1 WGCNA prognosis immunity therapy |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.919637/full |
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