Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo Study
The aim of this study was to provide a beneficial treatment effect of mesenchymal stem cell products derived from the canine amniotic membrane (AM-MSC) on the complicated wound healing process in dogs. AM-MSCs were characterized in terms of morphology, phenotypic profile, and multilineage differenti...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/9/8214 |
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| author | Filip Humenik Marcela Maloveská Nikola Hudáková Patrícia Petroušková Zuzana Šufliarska Ľubica Horňáková Alexandra Valenčáková Martin Kožár Barbora Šišková Dagmar Mudroňová Martin Bartkovský Daša Čížková |
| author_facet | Filip Humenik Marcela Maloveská Nikola Hudáková Patrícia Petroušková Zuzana Šufliarska Ľubica Horňáková Alexandra Valenčáková Martin Kožár Barbora Šišková Dagmar Mudroňová Martin Bartkovský Daša Čížková |
| author_sort | Filip Humenik |
| collection | DOAJ |
| description | The aim of this study was to provide a beneficial treatment effect of mesenchymal stem cell products derived from the canine amniotic membrane (AM-MSC) on the complicated wound healing process in dogs. AM-MSCs were characterized in terms of morphology, phenotypic profile, and multilineage differentiation potential. The in vitro study of the effect of canine amniotic mesenchymal stem cell conditioned media (AMMSC-CM) on a primary skin fibroblast cell culture scratch assay showed a decrease in the measured scratch area of about 66.39% against the negative control (Dulbecco’s Modified Eagle’s Medium—32.55%) and the positive control (Dulbecco’s Modified Eagle’s Medium supplemented with FGF2, N2, B27, and EGF—82.077%) after 72 h treatment. In the experimental study, seven dogs with complicated nonhealing wounds were treated with a combination of antibiotics, NSAIDs, and local AMMSC-CM application. After 15 days of therapy, we observed a 98.47% reduction in the wound surface area as opposed to 57.135% in the control group treated by conventional therapy based on debridement of necrotic tissue, antibiotic therapy, pain management, and change of wound dressing. |
| first_indexed | 2024-03-11T04:15:49Z |
| format | Article |
| id | doaj.art-0d3556fa9af64f73972c3640ac360f70 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:15:49Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0d3556fa9af64f73972c3640ac360f702023-11-17T23:06:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-01249821410.3390/ijms24098214Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo StudyFilip Humenik0Marcela Maloveská1Nikola Hudáková2Patrícia Petroušková3Zuzana Šufliarska4Ľubica Horňáková5Alexandra Valenčáková6Martin Kožár7Barbora Šišková8Dagmar Mudroňová9Martin Bartkovský10Daša Čížková11Centre of Experimental and Clinical Regenerative Medicine, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaCentre of Experimental and Clinical Regenerative Medicine, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaCentre of Experimental and Clinical Regenerative Medicine, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaCentre of Experimental and Clinical Regenerative Medicine, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaCentre of Experimental and Clinical Regenerative Medicine, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaSmall Animal Clinic, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaSmall Animal Clinic, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaSmall Animal Clinic, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaSmall Animal Clinic, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaInstitute of Microbiology and Immunology, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaDepartment of Food Hygiene, Technology and Safety, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaCentre of Experimental and Clinical Regenerative Medicine, The University of Veterinary Medicine and Pharmacy in Kosice, 040 01 Kosice, SlovakiaThe aim of this study was to provide a beneficial treatment effect of mesenchymal stem cell products derived from the canine amniotic membrane (AM-MSC) on the complicated wound healing process in dogs. AM-MSCs were characterized in terms of morphology, phenotypic profile, and multilineage differentiation potential. The in vitro study of the effect of canine amniotic mesenchymal stem cell conditioned media (AMMSC-CM) on a primary skin fibroblast cell culture scratch assay showed a decrease in the measured scratch area of about 66.39% against the negative control (Dulbecco’s Modified Eagle’s Medium—32.55%) and the positive control (Dulbecco’s Modified Eagle’s Medium supplemented with FGF2, N2, B27, and EGF—82.077%) after 72 h treatment. In the experimental study, seven dogs with complicated nonhealing wounds were treated with a combination of antibiotics, NSAIDs, and local AMMSC-CM application. After 15 days of therapy, we observed a 98.47% reduction in the wound surface area as opposed to 57.135% in the control group treated by conventional therapy based on debridement of necrotic tissue, antibiotic therapy, pain management, and change of wound dressing.https://www.mdpi.com/1422-0067/24/9/8214mesenchymal stem cellamnionconditioned mediawound healingfibroblast |
| spellingShingle | Filip Humenik Marcela Maloveská Nikola Hudáková Patrícia Petroušková Zuzana Šufliarska Ľubica Horňáková Alexandra Valenčáková Martin Kožár Barbora Šišková Dagmar Mudroňová Martin Bartkovský Daša Čížková Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo Study International Journal of Molecular Sciences mesenchymal stem cell amnion conditioned media wound healing fibroblast |
| title | Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo Study |
| title_full | Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo Study |
| title_fullStr | Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo Study |
| title_full_unstemmed | Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo Study |
| title_short | Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo Study |
| title_sort | impact of canine amniotic mesenchymal stem cell conditioned media on the wound healing process in vitro and in vivo study |
| topic | mesenchymal stem cell amnion conditioned media wound healing fibroblast |
| url | https://www.mdpi.com/1422-0067/24/9/8214 |
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