Introgression of tsv1 improves tungro disease resistance of a rice variety BRRI dhan71
Abstract Rice Tungro disease poses a threat to rice production in Asia. Marker assisted backcross breeding is the most feasible approach to address the tungro disease. We targeted to introgress tungro resistance locus tsv1 from Matatag 1 into a popular but tungro susceptible rice variety of Banglade...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-23413-4 |
| _version_ | 1797984734234542080 |
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| author | Tapas Kumer Hore Mary Ann Inabangan-asilo Ratna Wulandari Mohammad Abdul Latif Sheikh Arafat Islam Nihad Jose E. Hernandez Glenn B. Gregorio Teresita U. Dalisay Maria Genaleen Q. Diaz Balachiranjeevi Ch. B. P. Mallikarjuna Swamy |
| author_facet | Tapas Kumer Hore Mary Ann Inabangan-asilo Ratna Wulandari Mohammad Abdul Latif Sheikh Arafat Islam Nihad Jose E. Hernandez Glenn B. Gregorio Teresita U. Dalisay Maria Genaleen Q. Diaz Balachiranjeevi Ch. B. P. Mallikarjuna Swamy |
| author_sort | Tapas Kumer Hore |
| collection | DOAJ |
| description | Abstract Rice Tungro disease poses a threat to rice production in Asia. Marker assisted backcross breeding is the most feasible approach to address the tungro disease. We targeted to introgress tungro resistance locus tsv1 from Matatag 1 into a popular but tungro susceptible rice variety of Bangladesh, BRRI dhan71. The tsv1 locus was traced using two tightly linked markers RM336 and RM21801, and background genotyping was carried out using 7 K SNPs. A series of three back crosses followed by selfing resulted in identification of plants similar to BRRI dhan71. The background recovery varied at 91–95% with most of the lines having 95%. The disease screening of the lines showed moderate to high level of tungro resistance with a disease index score of ≤ 5. Introgression Lines (ILs) had medium slender grain type, and head rice recovery (59.2%), amylose content (20.1%), gel consistency (40.1 mm) and gelatinization temperature were within the acceptable range. AMMI and Kang’s stability analysis based on multi-location data revealed that multiple selected ILs outperformed BRRI dhan71 across the locations. IR144480-2-2-5, IR144483-1-2-4, IR144484-1-2-2 and IR144484-1-2-5 are the most promising lines. These lines will be further evaluated and nominated for varietal testing in Bangladesh. |
| first_indexed | 2024-04-11T07:06:22Z |
| format | Article |
| id | doaj.art-0d35bca47812425698e844e7585aca32 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T07:06:22Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0d35bca47812425698e844e7585aca322022-12-22T04:38:22ZengNature PortfolioScientific Reports2045-23222022-11-0112111410.1038/s41598-022-23413-4Introgression of tsv1 improves tungro disease resistance of a rice variety BRRI dhan71Tapas Kumer Hore0Mary Ann Inabangan-asilo1Ratna Wulandari2Mohammad Abdul Latif3Sheikh Arafat Islam Nihad4Jose E. Hernandez5Glenn B. Gregorio6Teresita U. Dalisay7Maria Genaleen Q. Diaz8Balachiranjeevi Ch.9B. P. Mallikarjuna Swamy10International Rice Research Institute (IRRI)International Rice Research Institute (IRRI)International Rice Research Institute (IRRI)Bangladesh Rice Research Institute (BRRI)Bangladesh Rice Research Institute (BRRI)University of the Philippines Los Banos (UPLB), CollegeUniversity of the Philippines Los Banos (UPLB), CollegeUniversity of the Philippines Los Banos (UPLB), CollegeUniversity of the Philippines Los Banos (UPLB), CollegeInternational Rice Research Institute (IRRI)International Rice Research Institute (IRRI)Abstract Rice Tungro disease poses a threat to rice production in Asia. Marker assisted backcross breeding is the most feasible approach to address the tungro disease. We targeted to introgress tungro resistance locus tsv1 from Matatag 1 into a popular but tungro susceptible rice variety of Bangladesh, BRRI dhan71. The tsv1 locus was traced using two tightly linked markers RM336 and RM21801, and background genotyping was carried out using 7 K SNPs. A series of three back crosses followed by selfing resulted in identification of plants similar to BRRI dhan71. The background recovery varied at 91–95% with most of the lines having 95%. The disease screening of the lines showed moderate to high level of tungro resistance with a disease index score of ≤ 5. Introgression Lines (ILs) had medium slender grain type, and head rice recovery (59.2%), amylose content (20.1%), gel consistency (40.1 mm) and gelatinization temperature were within the acceptable range. AMMI and Kang’s stability analysis based on multi-location data revealed that multiple selected ILs outperformed BRRI dhan71 across the locations. IR144480-2-2-5, IR144483-1-2-4, IR144484-1-2-2 and IR144484-1-2-5 are the most promising lines. These lines will be further evaluated and nominated for varietal testing in Bangladesh.https://doi.org/10.1038/s41598-022-23413-4 |
| spellingShingle | Tapas Kumer Hore Mary Ann Inabangan-asilo Ratna Wulandari Mohammad Abdul Latif Sheikh Arafat Islam Nihad Jose E. Hernandez Glenn B. Gregorio Teresita U. Dalisay Maria Genaleen Q. Diaz Balachiranjeevi Ch. B. P. Mallikarjuna Swamy Introgression of tsv1 improves tungro disease resistance of a rice variety BRRI dhan71 Scientific Reports |
| title | Introgression of tsv1 improves tungro disease resistance of a rice variety BRRI dhan71 |
| title_full | Introgression of tsv1 improves tungro disease resistance of a rice variety BRRI dhan71 |
| title_fullStr | Introgression of tsv1 improves tungro disease resistance of a rice variety BRRI dhan71 |
| title_full_unstemmed | Introgression of tsv1 improves tungro disease resistance of a rice variety BRRI dhan71 |
| title_short | Introgression of tsv1 improves tungro disease resistance of a rice variety BRRI dhan71 |
| title_sort | introgression of tsv1 improves tungro disease resistance of a rice variety brri dhan71 |
| url | https://doi.org/10.1038/s41598-022-23413-4 |
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