Analysis of Microbial Diversity and Community Structure of Rhizosphere Soil of Three <i>Astragalus</i> Species Grown in Special High-Cold Environment of Northwestern Yunnan, China
<i>Astragalus</i> is a medicinal plant with obvious rhizosphere effects. At present, there are many <i>Astragalus</i> plants with high application value but low recognition and resource reserves in the northwestern area of Yunnan province, China. In this study, metagenomics w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/3/539 |
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| author | Jia-Jie Ding Guo-Jun Zhou Xiao-Jie Chen Wei Xu Xing-Mei Gao Yong-Zeng Zhang Bei Jiang Hai-Feng Li Kai-Ling Wang |
| author_facet | Jia-Jie Ding Guo-Jun Zhou Xiao-Jie Chen Wei Xu Xing-Mei Gao Yong-Zeng Zhang Bei Jiang Hai-Feng Li Kai-Ling Wang |
| author_sort | Jia-Jie Ding |
| collection | DOAJ |
| description | <i>Astragalus</i> is a medicinal plant with obvious rhizosphere effects. At present, there are many <i>Astragalus</i> plants with high application value but low recognition and resource reserves in the northwestern area of Yunnan province, China. In this study, metagenomics was used to analyze the microbial diversity and community structure of rhizosphere soil of <i>A. forrestii</i>, <i>A. acaulis</i>, and <i>A. ernestii</i> plants grown in a special high-cold environment of northwestern Yunnan, China, at different altitudes ranging from 3225 to 4353 m. These microbes were taxonomically annotated to obtain 24 phyla and 501 genera for <i>A. forrestii</i>, 30 phyla and 504 genera for <i>A. acaulis</i>, as well as 39 phyla and 533 genera for <i>A. ernestii</i>. Overall, the dominant bacterial phyla included Proteobacteria, Actinobacteria, and Acidobacteria, while the dominant fungal ones were Ascomycota and Basidiomycota. At the genus level, <i>Bradyrhizobium</i>, <i>Afipia</i>, and <i>Paraburkholderia</i> were the most prevalent bacteria, and <i>Hyaloscypha</i>, <i>Pseudogymnoascus</i>, and <i>Russula</i> were the dominant fungal genera. Some of them are considered biocontrol microbes that could sustain the growth and health of host <i>Astragalus</i> plants. Redundancy analysis revealed that pH, TN, and SOM had a significant impact on the microbial community structures (<i>p</i> < 0.05). Finally, triterpene, flavonoid, polysaccharide, and amino acid metabolisms accounted for a high proportion of the enriched KEGG pathways, which possibly contributed to the synthesis of bioactive constituents in the <i>Astragalus</i> plants. |
| first_indexed | 2024-04-24T17:58:58Z |
| format | Article |
| id | doaj.art-0d3be9de446e4de1b1cee09976769974 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-24T17:58:58Z |
| publishDate | 2024-03-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0d3be9de446e4de1b1cee099767699742024-03-27T13:55:45ZengMDPI AGMicroorganisms2076-26072024-03-0112353910.3390/microorganisms12030539Analysis of Microbial Diversity and Community Structure of Rhizosphere Soil of Three <i>Astragalus</i> Species Grown in Special High-Cold Environment of Northwestern Yunnan, ChinaJia-Jie Ding0Guo-Jun Zhou1Xiao-Jie Chen2Wei Xu3Xing-Mei Gao4Yong-Zeng Zhang5Bei Jiang6Hai-Feng Li7Kai-Ling Wang8Yunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan, Dali 671000, ChinaYunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan, Dali 671000, ChinaYunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan, Dali 671000, ChinaYunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan, Dali 671000, ChinaYunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan, Dali 671000, ChinaYunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan, Dali 671000, ChinaYunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan, Dali 671000, ChinaYunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan, Dali 671000, ChinaYunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan, Dali 671000, China<i>Astragalus</i> is a medicinal plant with obvious rhizosphere effects. At present, there are many <i>Astragalus</i> plants with high application value but low recognition and resource reserves in the northwestern area of Yunnan province, China. In this study, metagenomics was used to analyze the microbial diversity and community structure of rhizosphere soil of <i>A. forrestii</i>, <i>A. acaulis</i>, and <i>A. ernestii</i> plants grown in a special high-cold environment of northwestern Yunnan, China, at different altitudes ranging from 3225 to 4353 m. These microbes were taxonomically annotated to obtain 24 phyla and 501 genera for <i>A. forrestii</i>, 30 phyla and 504 genera for <i>A. acaulis</i>, as well as 39 phyla and 533 genera for <i>A. ernestii</i>. Overall, the dominant bacterial phyla included Proteobacteria, Actinobacteria, and Acidobacteria, while the dominant fungal ones were Ascomycota and Basidiomycota. At the genus level, <i>Bradyrhizobium</i>, <i>Afipia</i>, and <i>Paraburkholderia</i> were the most prevalent bacteria, and <i>Hyaloscypha</i>, <i>Pseudogymnoascus</i>, and <i>Russula</i> were the dominant fungal genera. Some of them are considered biocontrol microbes that could sustain the growth and health of host <i>Astragalus</i> plants. Redundancy analysis revealed that pH, TN, and SOM had a significant impact on the microbial community structures (<i>p</i> < 0.05). Finally, triterpene, flavonoid, polysaccharide, and amino acid metabolisms accounted for a high proportion of the enriched KEGG pathways, which possibly contributed to the synthesis of bioactive constituents in the <i>Astragalus</i> plants.https://www.mdpi.com/2076-2607/12/3/539<i>Astragalus</i>rhizosphere microbiomespecial high-cold environmentnorthwestern Yunnan, Chinametagenomics |
| spellingShingle | Jia-Jie Ding Guo-Jun Zhou Xiao-Jie Chen Wei Xu Xing-Mei Gao Yong-Zeng Zhang Bei Jiang Hai-Feng Li Kai-Ling Wang Analysis of Microbial Diversity and Community Structure of Rhizosphere Soil of Three <i>Astragalus</i> Species Grown in Special High-Cold Environment of Northwestern Yunnan, China Microorganisms <i>Astragalus</i> rhizosphere microbiome special high-cold environment northwestern Yunnan, China metagenomics |
| title | Analysis of Microbial Diversity and Community Structure of Rhizosphere Soil of Three <i>Astragalus</i> Species Grown in Special High-Cold Environment of Northwestern Yunnan, China |
| title_full | Analysis of Microbial Diversity and Community Structure of Rhizosphere Soil of Three <i>Astragalus</i> Species Grown in Special High-Cold Environment of Northwestern Yunnan, China |
| title_fullStr | Analysis of Microbial Diversity and Community Structure of Rhizosphere Soil of Three <i>Astragalus</i> Species Grown in Special High-Cold Environment of Northwestern Yunnan, China |
| title_full_unstemmed | Analysis of Microbial Diversity and Community Structure of Rhizosphere Soil of Three <i>Astragalus</i> Species Grown in Special High-Cold Environment of Northwestern Yunnan, China |
| title_short | Analysis of Microbial Diversity and Community Structure of Rhizosphere Soil of Three <i>Astragalus</i> Species Grown in Special High-Cold Environment of Northwestern Yunnan, China |
| title_sort | analysis of microbial diversity and community structure of rhizosphere soil of three i astragalus i species grown in special high cold environment of northwestern yunnan china |
| topic | <i>Astragalus</i> rhizosphere microbiome special high-cold environment northwestern Yunnan, China metagenomics |
| url | https://www.mdpi.com/2076-2607/12/3/539 |
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