Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus
Abstract Background Pelvic floor muscles (PFM) and rectus abdominis muscles (RAM) of pregnant diabetic rats exhibit atrophy, co-localization of fast and slow fibers and an increased collagen type I/III ratio. However, the role of similar PFM or RAM hyperglycemic-related myopathy in women with gestat...
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-02-01
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| Series: | BMC Pregnancy and Childbirth |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12884-020-2749-x |
| _version_ | 1819016305129291776 |
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| author | Marilza V. C. Rudge Fátima P. Souza Joelcio F. Abbade Raghavendra L. S. Hallur João Paulo C. Marcondes Fernanda Piculo Gabriela Marini Giovana Vesentini Lehana Thabane Steven S. Witkin Iracema M. P. Calderon Angélica M. P. Barbosa The Diamater Study Group M. V. Rudge A. M. P. Barbosa I. M. P. Calderon F. P. Souza J. F. Abbade L. S. R. Hallur F. Piculo G. Marini G. Vesentini L. Thabane M. S. Palma C. F. O. Graeff R. K. Arni R. D. Herculano D. F. Salvadori S. Mateus M. Dal Pai Silva C. G. Magalhães R. A. Costa S. A. M. Lima S. L. Felisbino W. Barbosa A. Atallah M. J. B. Girão Z. Di Bella S. M. Uchoa S. Payão A. Hijas B. Berghman R. De Bie L. Sobrevia B. Junginger F. C. B. Alves P. S. Rossignoli C. B. Prudencio M. I. G. Orlandi M. I. Gonçalves S. K. Nunes B. B. Catinelli S. Quiroz B. V. Sarmento F. A. Pinheiro C. I. Sartorão R. R. Lucas D. R. A. Reyes S. B. C. V. Quiroz E. M. A. Enriquez R. G. Oliveira J. F. Floriano J. P. C. Marcondes S. Barneze T. D. Dangió T. Pascon P. Rossignoli J. V. Freitas L. Takano F. Reis T. D. Caldeirão J. N. Fernandes A. M. Carr M. V. C. Gaitero J. E. Corrente H. R. C. Nunes A. F. Candido S. M. B. Costa T. D. Dangió T. Pascon J. V. F. Melo L. Takano F. V. D. S. Reis T. D. Caldeirão A. M. Carr G. A. Garcia G. B. Rabadan H. C. M. Bassin K. S. Suyama L. N. Damasceno M. L. S. Takemoto M. D. Menezes D. G. Bussaneli V. K. C. Nogueira P. R. Lima I. O. Lourenço J. Marostica de Sá R. A. Megid I. P. Caruso L. T. Rasmussen G. M. Prata F. Piculo G. Vesentini M. A. Arantes G. A. R. Ferraz L. P. Camargo M. R. Kron J. E. Corrente H. R. C. Nunes |
| author_facet | Marilza V. C. Rudge Fátima P. Souza Joelcio F. Abbade Raghavendra L. S. Hallur João Paulo C. Marcondes Fernanda Piculo Gabriela Marini Giovana Vesentini Lehana Thabane Steven S. Witkin Iracema M. P. Calderon Angélica M. P. Barbosa The Diamater Study Group M. V. Rudge A. M. P. Barbosa I. M. P. Calderon F. P. Souza J. F. Abbade L. S. R. Hallur F. Piculo G. Marini G. Vesentini L. Thabane M. S. Palma C. F. O. Graeff R. K. Arni R. D. Herculano D. F. Salvadori S. Mateus M. Dal Pai Silva C. G. Magalhães R. A. Costa S. A. M. Lima S. L. Felisbino W. Barbosa A. Atallah M. J. B. Girão Z. Di Bella S. M. Uchoa S. Payão A. Hijas B. Berghman R. De Bie L. Sobrevia B. Junginger F. C. B. Alves P. S. Rossignoli C. B. Prudencio M. I. G. Orlandi M. I. Gonçalves S. K. Nunes B. B. Catinelli S. Quiroz B. V. Sarmento F. A. Pinheiro C. I. Sartorão R. R. Lucas D. R. A. Reyes S. B. C. V. Quiroz E. M. A. Enriquez R. G. Oliveira J. F. Floriano J. P. C. Marcondes S. Barneze T. D. Dangió T. Pascon P. Rossignoli J. V. Freitas L. Takano F. Reis T. D. Caldeirão J. N. Fernandes A. M. Carr M. V. C. Gaitero J. E. Corrente H. R. C. Nunes A. F. Candido S. M. B. Costa T. D. Dangió T. Pascon J. V. F. Melo L. Takano F. V. D. S. Reis T. D. Caldeirão A. M. Carr G. A. Garcia G. B. Rabadan H. C. M. Bassin K. S. Suyama L. N. Damasceno M. L. S. Takemoto M. D. Menezes D. G. Bussaneli V. K. C. Nogueira P. R. Lima I. O. Lourenço J. Marostica de Sá R. A. Megid I. P. Caruso L. T. Rasmussen G. M. Prata F. Piculo G. Vesentini M. A. Arantes G. A. R. Ferraz L. P. Camargo M. R. Kron J. E. Corrente H. R. C. Nunes |
| author_sort | Marilza V. C. Rudge |
| collection | DOAJ |
| description | Abstract Background Pelvic floor muscles (PFM) and rectus abdominis muscles (RAM) of pregnant diabetic rats exhibit atrophy, co-localization of fast and slow fibers and an increased collagen type I/III ratio. However, the role of similar PFM or RAM hyperglycemic-related myopathy in women with gestational diabetes mellitus (GDM) remains poorly investigated. This study aims to assess the frequency of pelvic floor muscle disorders and pregnancy-specific urinary incontinence (PS-UI) 12 months after the Cesarean (C) section in women with GDM. Specifically, differences in PFM/RAM hyperglycemic myopathy will be evaluated. Methods The Diamater is an ongoing cohort study of four groups of 59 pregnant women each from the Perinatal Diabetes Research Centre (PDRC), Botucatu Medical School (FMB)-UNESP (São Paulo State University), Brazil. Diagnosis of GDM and PS-UI will be made at 24–26 weeks, with a follow-up at 34–38 weeks of gestation. Inclusion in the study will occur at the time of C-section, and patients will be followed at 24–48 h, 6 weeks and 6 and 12 months postpartum. Study groups will be classified as (1) GDM plus PS-UI; (2) GDM without PS-UI; (3) Non-GDM plus PS-UI; and (4) Non-GDM without PS-UI. We will analyze relationships between GDM, PS-UI and hyperglycemic myopathy at 12 months after C-section. The mediator variables to be evaluated include digital palpation, vaginal squeeze pressure, 3D pelvic floor ultrasound, and 3D RAM ultrasound. RAM samples obtained during C-section will be analyzed for ex-vivo contractility, morphological, molecular and OMICS profiles to further characterize the hyperglycemic myopathy. Additional variables to be evaluated include maternal age, socioeconomic status, educational level, ethnicity, body mass index, weight gain during pregnancy, quality of glycemic control and insulin therapy. Discussion To our knowledge, this will be the first study to provide data on the prevalence of PS-UI and RAM and PFM physical and biomolecular muscle profiles after C-section in mothers with GDM. The longitudinal design allows for the assessment of cause-effect relationships between GDM, PS-UI, and PFMs and RAMs myopathy. The findings may reveal previously undetermined consequences of GDM. |
| first_indexed | 2024-12-21T02:45:31Z |
| format | Article |
| id | doaj.art-0d3f99d86d9a433a8d562ed3ae0e852a |
| institution | Directory Open Access Journal |
| issn | 1471-2393 |
| language | English |
| last_indexed | 2024-12-21T02:45:31Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pregnancy and Childbirth |
| spelling | doaj.art-0d3f99d86d9a433a8d562ed3ae0e852a2022-12-21T19:18:34ZengBMCBMC Pregnancy and Childbirth1471-23932020-02-0120111410.1186/s12884-020-2749-xStudy protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitusMarilza V. C. Rudge0Fátima P. Souza1Joelcio F. Abbade2Raghavendra L. S. Hallur3João Paulo C. Marcondes4Fernanda Piculo5Gabriela Marini6Giovana Vesentini7Lehana Thabane8Steven S. Witkin9Iracema M. P. Calderon10Angélica M. P. Barbosa11The Diamater Study Group12M. V. RudgeA. M. P. BarbosaI. M. P. CalderonF. P. SouzaJ. F. AbbadeL. S. R. HallurF. PiculoG. MariniG. VesentiniL. ThabaneM. S. PalmaC. F. O. GraeffR. K. ArniR. D. HerculanoD. F. SalvadoriS. MateusM. Dal Pai SilvaC. G. MagalhãesR. A. CostaS. A. M. LimaS. L. FelisbinoW. BarbosaA. AtallahM. J. B. GirãoZ. Di BellaS. M. UchoaS. PayãoA. HijasB. BerghmanR. De BieL. SobreviaB. JungingerF. C. B. AlvesP. S. RossignoliC. B. PrudencioM. I. G. OrlandiM. I. GonçalvesS. K. NunesB. B. CatinelliS. QuirozB. V. SarmentoF. A. PinheiroC. I. SartorãoR. R. LucasD. R. A. ReyesS. B. C. V. QuirozE. M. A. EnriquezR. G. OliveiraJ. F. FlorianoJ. P. C. MarcondesS. BarnezeT. D. DangióT. PasconP. RossignoliJ. V. FreitasL. TakanoF. ReisT. D. CaldeirãoJ. N. FernandesA. M. CarrM. V. C. GaiteroJ. E. CorrenteH. R. C. NunesA. F. CandidoS. M. B. CostaT. D. DangióT. PasconJ. V. F. MeloL. TakanoF. V. D. S. ReisT. D. CaldeirãoA. M. CarrG. A. GarciaG. B. RabadanH. C. M. BassinK. S. SuyamaL. N. DamascenoM. L. S. TakemotoM. D. MenezesD. G. BussaneliV. K. C. NogueiraP. R. LimaI. O. LourençoJ. Marostica de SáR. A. MegidI. P. CarusoL. T. RasmussenG. M. PrataF. PiculoG. VesentiniM. A. ArantesG. A. R. FerrazL. P. CamargoM. R. KronJ. E. CorrenteH. R. C. NunesDepartment of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP)Physics Department, Institute of Biosciences, Letters and Exact Sciences, Multiuser Center for Biomolecular Innovation, UNESP-São Paulo State UniversityDepartment of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP)Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP)Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP)Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP)Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP)Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP)Department of Health Research Methods, Evidence, and Impact, McMaster UniversityDepartment of Obstetrics and Gynecology, Weill Cornell MedicineDepartment of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP)Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP)Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP)Abstract Background Pelvic floor muscles (PFM) and rectus abdominis muscles (RAM) of pregnant diabetic rats exhibit atrophy, co-localization of fast and slow fibers and an increased collagen type I/III ratio. However, the role of similar PFM or RAM hyperglycemic-related myopathy in women with gestational diabetes mellitus (GDM) remains poorly investigated. This study aims to assess the frequency of pelvic floor muscle disorders and pregnancy-specific urinary incontinence (PS-UI) 12 months after the Cesarean (C) section in women with GDM. Specifically, differences in PFM/RAM hyperglycemic myopathy will be evaluated. Methods The Diamater is an ongoing cohort study of four groups of 59 pregnant women each from the Perinatal Diabetes Research Centre (PDRC), Botucatu Medical School (FMB)-UNESP (São Paulo State University), Brazil. Diagnosis of GDM and PS-UI will be made at 24–26 weeks, with a follow-up at 34–38 weeks of gestation. Inclusion in the study will occur at the time of C-section, and patients will be followed at 24–48 h, 6 weeks and 6 and 12 months postpartum. Study groups will be classified as (1) GDM plus PS-UI; (2) GDM without PS-UI; (3) Non-GDM plus PS-UI; and (4) Non-GDM without PS-UI. We will analyze relationships between GDM, PS-UI and hyperglycemic myopathy at 12 months after C-section. The mediator variables to be evaluated include digital palpation, vaginal squeeze pressure, 3D pelvic floor ultrasound, and 3D RAM ultrasound. RAM samples obtained during C-section will be analyzed for ex-vivo contractility, morphological, molecular and OMICS profiles to further characterize the hyperglycemic myopathy. Additional variables to be evaluated include maternal age, socioeconomic status, educational level, ethnicity, body mass index, weight gain during pregnancy, quality of glycemic control and insulin therapy. Discussion To our knowledge, this will be the first study to provide data on the prevalence of PS-UI and RAM and PFM physical and biomolecular muscle profiles after C-section in mothers with GDM. The longitudinal design allows for the assessment of cause-effect relationships between GDM, PS-UI, and PFMs and RAMs myopathy. The findings may reveal previously undetermined consequences of GDM.http://link.springer.com/article/10.1186/s12884-020-2749-xGestational diabetes mellitusHyperglycemic myopathyPelvic floor musclesRectus abdominis musclesUrinary incontinenceProteomics |
| spellingShingle | Marilza V. C. Rudge Fátima P. Souza Joelcio F. Abbade Raghavendra L. S. Hallur João Paulo C. Marcondes Fernanda Piculo Gabriela Marini Giovana Vesentini Lehana Thabane Steven S. Witkin Iracema M. P. Calderon Angélica M. P. Barbosa The Diamater Study Group M. V. Rudge A. M. P. Barbosa I. M. P. Calderon F. P. Souza J. F. Abbade L. S. R. Hallur F. Piculo G. Marini G. Vesentini L. Thabane M. S. Palma C. F. O. Graeff R. K. Arni R. D. Herculano D. F. Salvadori S. Mateus M. Dal Pai Silva C. G. Magalhães R. A. Costa S. A. M. Lima S. L. Felisbino W. Barbosa A. Atallah M. J. B. Girão Z. Di Bella S. M. Uchoa S. Payão A. Hijas B. Berghman R. De Bie L. Sobrevia B. Junginger F. C. B. Alves P. S. Rossignoli C. B. Prudencio M. I. G. Orlandi M. I. Gonçalves S. K. Nunes B. B. Catinelli S. Quiroz B. V. Sarmento F. A. Pinheiro C. I. Sartorão R. R. Lucas D. R. A. Reyes S. B. C. V. Quiroz E. M. A. Enriquez R. G. Oliveira J. F. Floriano J. P. C. Marcondes S. Barneze T. D. Dangió T. Pascon P. Rossignoli J. V. Freitas L. Takano F. Reis T. D. Caldeirão J. N. Fernandes A. M. Carr M. V. C. Gaitero J. E. Corrente H. R. C. Nunes A. F. Candido S. M. B. Costa T. D. Dangió T. Pascon J. V. F. Melo L. Takano F. V. D. S. Reis T. D. Caldeirão A. M. Carr G. A. Garcia G. B. Rabadan H. C. M. Bassin K. S. Suyama L. N. Damasceno M. L. S. Takemoto M. D. Menezes D. G. Bussaneli V. K. C. Nogueira P. R. Lima I. O. Lourenço J. Marostica de Sá R. A. Megid I. P. Caruso L. T. Rasmussen G. M. Prata F. Piculo G. Vesentini M. A. Arantes G. A. R. Ferraz L. P. Camargo M. R. Kron J. E. Corrente H. R. C. Nunes Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus BMC Pregnancy and Childbirth Gestational diabetes mellitus Hyperglycemic myopathy Pelvic floor muscles Rectus abdominis muscles Urinary incontinence Proteomics |
| title | Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
| title_full | Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
| title_fullStr | Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
| title_full_unstemmed | Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
| title_short | Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
| title_sort | study protocol to investigate biomolecular muscle profile as predictors of long term urinary incontinence in women with gestational diabetes mellitus |
| topic | Gestational diabetes mellitus Hyperglycemic myopathy Pelvic floor muscles Rectus abdominis muscles Urinary incontinence Proteomics |
| url | http://link.springer.com/article/10.1186/s12884-020-2749-x |
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amcarr studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT mvcgaitero studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT jecorrente studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT hrcnunes studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT afcandido studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT smbcosta studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT tddangio studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT tpascon studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT jvfmelo studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT ltakano studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT fvdsreis studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT tdcaldeirao studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT amcarr studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT gagarcia studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT gbrabadan studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT hcmbassin studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT kssuyama studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT lndamasceno studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT mlstakemoto studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT mdmenezes studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT dgbussaneli studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT vkcnogueira studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT prlima studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT iolourenco studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT jmarosticadesa studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT ramegid studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT ipcaruso studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT ltrasmussen studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT gmprata studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT fpiculo studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT gvesentini studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT maarantes studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT garferraz studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT lpcamargo studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT mrkron studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT jecorrente studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus AT hrcnunes studyprotocoltoinvestigatebiomolecularmuscleprofileaspredictorsoflongtermurinaryincontinenceinwomenwithgestationaldiabetesmellitus |