Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to Pathogens
In the present scenario of a looming food crisis, improving per hectare rice productivity at a greater pace is among the topmost priorities of scientists and breeders. In the past decades, conventional, mutational, and marker-assisted breeding techniques have played a significant role in developing...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/3/565 |
| _version_ | 1797473331975290880 |
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| author | Anirudha Chattopadhyay Jyotika Purohit Sahil Mehta Hemangini Parmar Sangeetha Karippadakam Afreen Rashid Alexander Balamurugan Shilpi Bansal Ganesan Prakash V. Mohan Murali Achary Malireddy K. Reddy |
| author_facet | Anirudha Chattopadhyay Jyotika Purohit Sahil Mehta Hemangini Parmar Sangeetha Karippadakam Afreen Rashid Alexander Balamurugan Shilpi Bansal Ganesan Prakash V. Mohan Murali Achary Malireddy K. Reddy |
| author_sort | Anirudha Chattopadhyay |
| collection | DOAJ |
| description | In the present scenario of a looming food crisis, improving per hectare rice productivity at a greater pace is among the topmost priorities of scientists and breeders. In the past decades, conventional, mutational, and marker-assisted breeding techniques have played a significant role in developing multiple desired rice varieties. However, due to certain limitations, these techniques cannot furnish the projected food security of the 2050 population’s aching stomachs. One of the possible options would be precise crop genome editing using various tools, <i>viz.</i>, TALENs and CRISPR/Cas9 to resolve this multifaceted crisis. Initially, the potentiality of these technologies was tested only in the rice protoplasts. Later, the techniques were employed to edit calli with help of modified vectors, CRISPR variants, cassette cloning systems, and delivery methods. With the continuous technological advancements such as base editing, multiplexing, etc., the precision, rapidness, efficiency, reliability, potency, and range of applications of these platforms have increased and even been used for gene function studies. This leads to a revolution in the field of the rice improvement program, especially the stress tolerance against various pests and pathogens in which the susceptibility factors located within the rice genome are targeted through genome editing tools. Therefore, in this current article, we have summarized the advancements in the rice genome editing tools during the last decade concerning enhanced biotic stress tolerance. Additionally, we have focused on the regulatory aspects of genome editing with associated risks and limitations, and the prospects to reshape the rice genome for durable resistance to complex biotic stress. |
| first_indexed | 2024-03-09T20:13:15Z |
| format | Article |
| id | doaj.art-0d410d52cd10408ca77c6c0395a0ae92 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T20:13:15Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-0d410d52cd10408ca77c6c0395a0ae922023-11-24T00:07:00ZengMDPI AGAgronomy2073-43952022-02-0112356510.3390/agronomy12030565Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to PathogensAnirudha Chattopadhyay0Jyotika Purohit1Sahil Mehta2Hemangini Parmar3Sangeetha Karippadakam4Afreen Rashid5Alexander Balamurugan6Shilpi Bansal7Ganesan Prakash8V. Mohan Murali Achary9Malireddy K. Reddy10Pulses Research Station, Department of Plant Pathology, SD Agricultural University, Palanpur 390003, IndiaDepartment of Plant Pathology, C. P. College of Agriculture, SD Agricultural University, Palanpur 385505, IndiaCrop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, IndiaCrop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, IndiaCrop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, IndiaCrop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, IndiaDivision of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaCrop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, IndiaCrop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, IndiaIn the present scenario of a looming food crisis, improving per hectare rice productivity at a greater pace is among the topmost priorities of scientists and breeders. In the past decades, conventional, mutational, and marker-assisted breeding techniques have played a significant role in developing multiple desired rice varieties. However, due to certain limitations, these techniques cannot furnish the projected food security of the 2050 population’s aching stomachs. One of the possible options would be precise crop genome editing using various tools, <i>viz.</i>, TALENs and CRISPR/Cas9 to resolve this multifaceted crisis. Initially, the potentiality of these technologies was tested only in the rice protoplasts. Later, the techniques were employed to edit calli with help of modified vectors, CRISPR variants, cassette cloning systems, and delivery methods. With the continuous technological advancements such as base editing, multiplexing, etc., the precision, rapidness, efficiency, reliability, potency, and range of applications of these platforms have increased and even been used for gene function studies. This leads to a revolution in the field of the rice improvement program, especially the stress tolerance against various pests and pathogens in which the susceptibility factors located within the rice genome are targeted through genome editing tools. Therefore, in this current article, we have summarized the advancements in the rice genome editing tools during the last decade concerning enhanced biotic stress tolerance. Additionally, we have focused on the regulatory aspects of genome editing with associated risks and limitations, and the prospects to reshape the rice genome for durable resistance to complex biotic stress.https://www.mdpi.com/2073-4395/12/3/565food securityricebiotic stressCRISPR/Cas systemssusceptibility gene |
| spellingShingle | Anirudha Chattopadhyay Jyotika Purohit Sahil Mehta Hemangini Parmar Sangeetha Karippadakam Afreen Rashid Alexander Balamurugan Shilpi Bansal Ganesan Prakash V. Mohan Murali Achary Malireddy K. Reddy Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to Pathogens Agronomy food security rice biotic stress CRISPR/Cas systems susceptibility gene |
| title | Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to Pathogens |
| title_full | Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to Pathogens |
| title_fullStr | Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to Pathogens |
| title_full_unstemmed | Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to Pathogens |
| title_short | Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to Pathogens |
| title_sort | precision genome editing toolbox applications and approaches for improving rice s genetic resistance to pathogens |
| topic | food security rice biotic stress CRISPR/Cas systems susceptibility gene |
| url | https://www.mdpi.com/2073-4395/12/3/565 |
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