The Increased RNase Activity of IRE1α in PBMCs from Patients with Rheumatoid Arthritis
Purpose: Despite recent advances in the diagnosis and treatment of rheumatoid arthritis (RA), this inflammatory disease remains a challenge to patients and physicians. Recent evidence highlights the contribution of endoplasmic reticulum (ER) stress in the pathogenesis and treatment of RA. Herein, we...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2019-08-01
|
| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | https://apb.tbzmed.ac.ir/PDF/apb-27554 |
| _version_ | 1818081732366172160 |
|---|---|
| author | Mahdieh Ahmadiany Mahshid Alavi-Samani Zahra Hashemi Mohammad Amin Moosavi Marveh Rahmati |
| author_facet | Mahdieh Ahmadiany Mahshid Alavi-Samani Zahra Hashemi Mohammad Amin Moosavi Marveh Rahmati |
| author_sort | Mahdieh Ahmadiany |
| collection | DOAJ |
| description | Purpose: Despite recent advances in the diagnosis and treatment of rheumatoid arthritis (RA), this inflammatory disease remains a challenge to patients and physicians. Recent evidence highlights the contribution of endoplasmic reticulum (ER) stress in the pathogenesis and treatment of RA. Herein, we study the expression of the ER stress sensor inositol-requiring enzyme 1α (IRE1α), as well as XBP1 splicing and the regulated IRE1-dependent decay (RIDD), in peripheral blood mononuclear cells (PBMCs) from patients with RA compared with healthy controls. Methods: The PBMCs from blood samples of RA patients and healthy volunteers were isolated by a density gradient centrifugation method using Ficoll. The gene expression levels of GRP78/ Bip, IRE1, XBP1s, micro-RNAs (miRNAs) were evaluated by real-time PCR. Results: The expression of GRP78, IRE1, and XBP1s were increased in PBMCs of RA patients compared with healthy controls. We further show that the RIDD targets (miRNA-17, -34a, -96, and -125b) were downregulated in RA samples. Conclusion: This study can expand our knowledge on the importance of RNase activity of IRE1α in RA and may offer new potentials for developing novel diagnostic and/or therapeutic biomarkers. |
| first_indexed | 2024-12-10T19:10:53Z |
| format | Article |
| id | doaj.art-0d4449dd106a43a1acd136edd6883f5d |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-10T19:10:53Z |
| publishDate | 2019-08-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-0d4449dd106a43a1acd136edd6883f5d2022-12-22T01:36:45ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082019-08-019350550910.15171/apb.2019.060apb-27554The Increased RNase Activity of IRE1α in PBMCs from Patients with Rheumatoid ArthritisMahdieh Ahmadiany0Mahshid Alavi-Samani1Zahra Hashemi2Mohammad Amin Moosavi3Marveh Rahmati4Cancer Biology Research Center, Tehran University of Medical Sciences, Tehran, Iran.Cancer Biology Research Center, Tehran University of Medical Sciences, Tehran, Iran.Department of Rheumatology, Imam Hossein Teaching Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran P.O Box 14965/161, Iran.Cancer Biology Research Center, Tehran University of Medical Sciences, Tehran, Iran.Purpose: Despite recent advances in the diagnosis and treatment of rheumatoid arthritis (RA), this inflammatory disease remains a challenge to patients and physicians. Recent evidence highlights the contribution of endoplasmic reticulum (ER) stress in the pathogenesis and treatment of RA. Herein, we study the expression of the ER stress sensor inositol-requiring enzyme 1α (IRE1α), as well as XBP1 splicing and the regulated IRE1-dependent decay (RIDD), in peripheral blood mononuclear cells (PBMCs) from patients with RA compared with healthy controls. Methods: The PBMCs from blood samples of RA patients and healthy volunteers were isolated by a density gradient centrifugation method using Ficoll. The gene expression levels of GRP78/ Bip, IRE1, XBP1s, micro-RNAs (miRNAs) were evaluated by real-time PCR. Results: The expression of GRP78, IRE1, and XBP1s were increased in PBMCs of RA patients compared with healthy controls. We further show that the RIDD targets (miRNA-17, -34a, -96, and -125b) were downregulated in RA samples. Conclusion: This study can expand our knowledge on the importance of RNase activity of IRE1α in RA and may offer new potentials for developing novel diagnostic and/or therapeutic biomarkers.https://apb.tbzmed.ac.ir/PDF/apb-27554Endoplasmic reticulum stressInositol-requiring enzyme 1IRE1-dependent decaymicroRNARheumatoid arthritis |
| spellingShingle | Mahdieh Ahmadiany Mahshid Alavi-Samani Zahra Hashemi Mohammad Amin Moosavi Marveh Rahmati The Increased RNase Activity of IRE1α in PBMCs from Patients with Rheumatoid Arthritis Advanced Pharmaceutical Bulletin Endoplasmic reticulum stress Inositol-requiring enzyme 1 IRE1-dependent decay microRNA Rheumatoid arthritis |
| title | The Increased RNase Activity of IRE1α in PBMCs from Patients with Rheumatoid Arthritis |
| title_full | The Increased RNase Activity of IRE1α in PBMCs from Patients with Rheumatoid Arthritis |
| title_fullStr | The Increased RNase Activity of IRE1α in PBMCs from Patients with Rheumatoid Arthritis |
| title_full_unstemmed | The Increased RNase Activity of IRE1α in PBMCs from Patients with Rheumatoid Arthritis |
| title_short | The Increased RNase Activity of IRE1α in PBMCs from Patients with Rheumatoid Arthritis |
| title_sort | increased rnase activity of ire1α in pbmcs from patients with rheumatoid arthritis |
| topic | Endoplasmic reticulum stress Inositol-requiring enzyme 1 IRE1-dependent decay microRNA Rheumatoid arthritis |
| url | https://apb.tbzmed.ac.ir/PDF/apb-27554 |
| work_keys_str_mv | AT mahdiehahmadiany theincreasedrnaseactivityofire1ainpbmcsfrompatientswithrheumatoidarthritis AT mahshidalavisamani theincreasedrnaseactivityofire1ainpbmcsfrompatientswithrheumatoidarthritis AT zahrahashemi theincreasedrnaseactivityofire1ainpbmcsfrompatientswithrheumatoidarthritis AT mohammadaminmoosavi theincreasedrnaseactivityofire1ainpbmcsfrompatientswithrheumatoidarthritis AT marvehrahmati theincreasedrnaseactivityofire1ainpbmcsfrompatientswithrheumatoidarthritis AT mahdiehahmadiany increasedrnaseactivityofire1ainpbmcsfrompatientswithrheumatoidarthritis AT mahshidalavisamani increasedrnaseactivityofire1ainpbmcsfrompatientswithrheumatoidarthritis AT zahrahashemi increasedrnaseactivityofire1ainpbmcsfrompatientswithrheumatoidarthritis AT mohammadaminmoosavi increasedrnaseactivityofire1ainpbmcsfrompatientswithrheumatoidarthritis AT marvehrahmati increasedrnaseactivityofire1ainpbmcsfrompatientswithrheumatoidarthritis |