New national conversion line for Bactoscan FC in Italy: A step forward
To improve the reproducibility of flow cytometry technique for total bacterial count in milk, a conversion from instrumental results (impulses/μL) to the reference method resultes (cfu/mL) is needed. In 2008 in Italy, a project for a common conversion line for Bactoscan FC was initiated.<br />...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Chiriotti Editori
2015-06-01
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| Series: | Italian Journal of Food Science |
| Subjects: | |
| Online Access: | http://www.chiriottieditori.it/ojs/index.php/ijfs/article/view/186 |
| _version_ | 1818420191177998336 |
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| author | G. Bolzoni A. Marcolini G. Delle Donne B. Appicciafuoco A.M. Ferrini |
| author_facet | G. Bolzoni A. Marcolini G. Delle Donne B. Appicciafuoco A.M. Ferrini |
| author_sort | G. Bolzoni |
| collection | DOAJ |
| description | To improve the reproducibility of flow cytometry technique for total bacterial count in milk, a conversion from instrumental results (impulses/μL) to the reference method resultes (cfu/mL) is needed. In 2008 in Italy, a project for a common conversion line for Bactoscan FC was initiated.<br />In this paper we report on the second phase of the project focusing on the statistical procedure used to evaluate the validity of the data. The new conversion line, representative of national milk<br />(2,732 valid samples from 29 labs) obtained from both rounds of the study is: Log10 (cfu mL-1) = Log10 (IBC μL-1) x 0.939 + 2.559, with S y:x= 0.282 with an application range up to 70,000 IBC μL-1. |
| first_indexed | 2024-12-14T12:50:32Z |
| format | Article |
| id | doaj.art-0d4563b7d2334512843a7bc07469fcf1 |
| institution | Directory Open Access Journal |
| issn | 1120-1770 1120-1770 |
| language | English |
| last_indexed | 2024-12-14T12:50:32Z |
| publishDate | 2015-06-01 |
| publisher | Chiriotti Editori |
| record_format | Article |
| series | Italian Journal of Food Science |
| spelling | doaj.art-0d4563b7d2334512843a7bc07469fcf12022-12-21T23:00:41ZengChiriotti EditoriItalian Journal of Food Science1120-17701120-17702015-06-0127219119710.14674/1120-1770/ijfs.v18628New national conversion line for Bactoscan FC in Italy: A step forwardG. Bolzoni0A. Marcolini1G. Delle Donne2B. Appicciafuoco3A.M. Ferrini4IZSLERIZSLER, BresciaIZSLER, BresciaIstituto Superiore di Sanità, RomaIstituto Superiore di Sanità, RomaTo improve the reproducibility of flow cytometry technique for total bacterial count in milk, a conversion from instrumental results (impulses/μL) to the reference method resultes (cfu/mL) is needed. In 2008 in Italy, a project for a common conversion line for Bactoscan FC was initiated.<br />In this paper we report on the second phase of the project focusing on the statistical procedure used to evaluate the validity of the data. The new conversion line, representative of national milk<br />(2,732 valid samples from 29 labs) obtained from both rounds of the study is: Log10 (cfu mL-1) = Log10 (IBC μL-1) x 0.939 + 2.559, with S y:x= 0.282 with an application range up to 70,000 IBC μL-1.http://www.chiriottieditori.it/ojs/index.php/ijfs/article/view/186Bactoscan FC, conversion line, cow milk, total bacterial count |
| spellingShingle | G. Bolzoni A. Marcolini G. Delle Donne B. Appicciafuoco A.M. Ferrini New national conversion line for Bactoscan FC in Italy: A step forward Italian Journal of Food Science Bactoscan FC, conversion line, cow milk, total bacterial count |
| title | New national conversion line for Bactoscan FC in Italy: A step forward |
| title_full | New national conversion line for Bactoscan FC in Italy: A step forward |
| title_fullStr | New national conversion line for Bactoscan FC in Italy: A step forward |
| title_full_unstemmed | New national conversion line for Bactoscan FC in Italy: A step forward |
| title_short | New national conversion line for Bactoscan FC in Italy: A step forward |
| title_sort | new national conversion line for bactoscan fc in italy a step forward |
| topic | Bactoscan FC, conversion line, cow milk, total bacterial count |
| url | http://www.chiriottieditori.it/ojs/index.php/ijfs/article/view/186 |
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