Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5
A popular polyherbal formulation prepared from five plants (PHF5) may have anticancer effects. However, there is a lack of adequate scientific evidence. We assessed the anticancer, antioxidant, and acute toxicity effects of PHF5. Cancer cells were treated with 0 to 300 μg/mL PHF5 extract. Establishe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2020-05-01
|
| Series: | Open Chemistry |
| Subjects: | |
| Online Access: | http://www.degruyter.com/view/j/chem.2020.18.issue-1/chem-2020-0047/chem-2020-0047.xml?format=INT |
| _version_ | 1819171476846149632 |
|---|---|
| author | Abutaha Nael Al-zharani Mohammed Al-Doaiss Amin A. Baabbad Almohannad Al-malki Ahmed Mfreh Dekhil Hafedh |
| author_facet | Abutaha Nael Al-zharani Mohammed Al-Doaiss Amin A. Baabbad Almohannad Al-malki Ahmed Mfreh Dekhil Hafedh |
| author_sort | Abutaha Nael |
| collection | DOAJ |
| description | A popular polyherbal formulation prepared from five plants (PHF5) may have anticancer effects. However, there is a lack of adequate scientific evidence. We assessed the anticancer, antioxidant, and acute toxicity effects of PHF5. Cancer cells were treated with 0 to 300 μg/mL PHF5 extract. Established assays were used to assess cytotoxicity, apoptosis, and radical scavenging activities. In the acute toxicity study, mice were administered a single oral dose (2,000 mg/kg) of PHF5, and biochemical and histopathological parameters were assessed. The IC50 values of PHF5 on LoVo, HepG2, MCF-7, and MDA-MB 231 cells were 71.8, 64.8, 45.3, and 47.3 μg/mL, respectively. Fluorescence staining demonstrated that PHF5 induced MCF-7 cell apoptosis. After 48 h, the percentage of late apoptotic cells increased significantly compared with the control cells (74.16 ± 0.64 vs 3.7 ± 2.05, P < 0.05). No mortality or behavioral alterations were observed in mice treated with a single dose (2,000 mg/kg) of PHF5, indicating that the LD50 value exceeded 2,000 mg/kg. However, histopathological changes were observed in the liver tissues. PHF5 has potential as a therapeutic agent for the treatment of human carcinoma. Further safety data will be necessary before clinical use. |
| first_indexed | 2024-12-22T19:51:54Z |
| format | Article |
| id | doaj.art-0d467062e3bc40dca938d154c312c0ba |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-12-22T19:51:54Z |
| publishDate | 2020-05-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-0d467062e3bc40dca938d154c312c0ba2022-12-21T18:14:31ZengDe GruyterOpen Chemistry2391-54202020-05-0118147248110.1515/chem-2020-0047chem-2020-0047Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5Abutaha Nael0Al-zharani Mohammed1Al-Doaiss Amin A.2Baabbad Almohannad3Al-malki Ahmed Mfreh4Dekhil Hafedh5Bioproducts Research Chair, Department of Zoology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11461, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), P. O. Box 90950, Riyadh 11461, Saudi ArabiaDepartment of Biology, College of Science, King Khalid University, Abha, Saudi ArabiaBioproducts Research Chair, Department of Zoology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11461, Saudi ArabiaBioproducts Research Chair, Department of Zoology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11461, Saudi ArabiaObesity Research Center, College of Medicine, King Saud University, P. O. Box 2925 (98), Riyadh 11461, Saudi ArabiaA popular polyherbal formulation prepared from five plants (PHF5) may have anticancer effects. However, there is a lack of adequate scientific evidence. We assessed the anticancer, antioxidant, and acute toxicity effects of PHF5. Cancer cells were treated with 0 to 300 μg/mL PHF5 extract. Established assays were used to assess cytotoxicity, apoptosis, and radical scavenging activities. In the acute toxicity study, mice were administered a single oral dose (2,000 mg/kg) of PHF5, and biochemical and histopathological parameters were assessed. The IC50 values of PHF5 on LoVo, HepG2, MCF-7, and MDA-MB 231 cells were 71.8, 64.8, 45.3, and 47.3 μg/mL, respectively. Fluorescence staining demonstrated that PHF5 induced MCF-7 cell apoptosis. After 48 h, the percentage of late apoptotic cells increased significantly compared with the control cells (74.16 ± 0.64 vs 3.7 ± 2.05, P < 0.05). No mortality or behavioral alterations were observed in mice treated with a single dose (2,000 mg/kg) of PHF5, indicating that the LD50 value exceeded 2,000 mg/kg. However, histopathological changes were observed in the liver tissues. PHF5 has potential as a therapeutic agent for the treatment of human carcinoma. Further safety data will be necessary before clinical use.http://www.degruyter.com/view/j/chem.2020.18.issue-1/chem-2020-0047/chem-2020-0047.xml?format=INTpolyherbal formulationanticancerapoptosisphytochemicalacute toxicity |
| spellingShingle | Abutaha Nael Al-zharani Mohammed Al-Doaiss Amin A. Baabbad Almohannad Al-malki Ahmed Mfreh Dekhil Hafedh Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5 Open Chemistry polyherbal formulation anticancer apoptosis phytochemical acute toxicity |
| title | Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5 |
| title_full | Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5 |
| title_fullStr | Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5 |
| title_full_unstemmed | Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5 |
| title_short | Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5 |
| title_sort | anticancer antioxidant and acute toxicity studies of a saudi polyherbal formulation phf5 |
| topic | polyherbal formulation anticancer apoptosis phytochemical acute toxicity |
| url | http://www.degruyter.com/view/j/chem.2020.18.issue-1/chem-2020-0047/chem-2020-0047.xml?format=INT |
| work_keys_str_mv | AT abutahanael anticancerantioxidantandacutetoxicitystudiesofasaudipolyherbalformulationphf5 AT alzharanimohammed anticancerantioxidantandacutetoxicitystudiesofasaudipolyherbalformulationphf5 AT aldoaissamina anticancerantioxidantandacutetoxicitystudiesofasaudipolyherbalformulationphf5 AT baabbadalmohannad anticancerantioxidantandacutetoxicitystudiesofasaudipolyherbalformulationphf5 AT almalkiahmedmfreh anticancerantioxidantandacutetoxicitystudiesofasaudipolyherbalformulationphf5 AT dekhilhafedh anticancerantioxidantandacutetoxicitystudiesofasaudipolyherbalformulationphf5 |