Fagraea racemosa leaf extract inhibits oxidative stress-induced liver damage in Wistar rats
<p><strong><em>Latar belakang</em></strong><em>: Kemampuan hati mengatasi stres oksidatif dapat ditingkatkan dengan konsumsi antioksidan eksogen yang berasal dari alam.</em><em> Penelitian ini ditujukan untuk mempelajari kemampuan hepatoprotektif dari...
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| Format: | Article |
| Language: | English |
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Badan Penelitian dan Pengembangan Kesehatan
2012-07-01
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| Series: | Health Science Journal of Indonesia |
| Subjects: | |
| Online Access: | http://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/63 |
| _version_ | 1819080633580781568 |
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| author | Eva Rachmi Yudanti Riastiti |
| author_facet | Eva Rachmi Yudanti Riastiti |
| author_sort | Eva Rachmi |
| collection | DOAJ |
| description | <p><strong><em>Latar belakang</em></strong><em>: Kemampuan hati mengatasi stres oksidatif dapat ditingkatkan dengan konsumsi antioksidan eksogen yang berasal dari alam.</em><em> Penelitian ini ditujukan untuk mempelajari kemampuan hepatoprotektif dari ekstrak metanol daun Fagraea racemosa, dengan menggunakan CCL<sub>4</sub> sebagai model sumber radikal bebas. </em></p> <p><strong><em>Metode</em></strong><em>: </em><em>Tiga kelompok perlakuan tikus Wistar (enam ekor per kelompok), masing-masing diberi</em><em> dosis ekstrak</em><em> berturut-turut 50,</em><em> </em><em>100, 200 mg/kg bb per oral, sekali perhari selama 30 hari. CCl<sub>4</sub> diinjeksikan intraperitoneal kepada ketiga kelompok , dua kali per minggu (1,5 ml/kg bb). Sebagai pembanding, digunakan dua kelompok kontrol, yaitu kontrol normal dan kontrol CCl<sub>4</sub>. Pada hari ke-30, tikus dibunuh dan hati diwarnai dengan hematoksilin-eosin. Perubahan histopatologi ditentukan berdasar derajat steatosis, degenerasi hidropik, dan inflamasi. Data dianalisis dengan Anova dan uji post hoc LSD (p≤0.05) menggunakan SPSS versi 13.0</em></p> <p><strong><em>Hasil:</em></strong><em> Hasil menunjukkan perbaikan derajat degenerasi hidropik dan inflamasi (p≤0,05) pada ketiga kelompok perlakuan bila dibanding dengan kelompok kontrol CCl<sub>4. </sub>Tetapi, derajat steatosis meningkat pada kelompok perlakuan dosis 50 dan 100 mg/kg bb, dan kemudian menurun secara bermakna pada perlakuan 200 mg/kg bb. </em></p> <p><strong><em>Kesimpulan :</em></strong><em> Ekstrak methanol daun </em><em>Fagraea racemosa mampu melindungi hati dari radikal bebas yang dihasilkan dari CCl<sub>4</sub>. Hasil ini mengindikasikan bahwa Fagraea racemosa menjanjikan untuk dikembangkan sebagai suplemen antioksidan. </em><strong><em>(</em></strong><strong><em>Health Science Indones 2011;2:46-51)</em></strong><em></em></p> <p> </p> <p><strong>Abstract </strong></p> <p><strong>Background</strong>: The ability of the liver in dealing with oxidative stress can be enhanced by consumption of exogenous antioxidants derived from nature. This study aimed to explore the hepatoprotective ability of <em>Fagraea racemosa</em> leaves methanolic extract against CCl<sub>4</sub> exposure as a model of free radicals source.</p> <p><strong>Methods</strong>: Three different doses (50, 100, 200 mg/kg bw) were administered orally to three treatment groups of Wistar rats (six Wistar rats each), respectively, once per day for 30 days. CCl<sub>4</sub> injected intraperitoneally to those three groups, twice a week (1,5 ml/kg bw). Two control groups were provided that were one normal control group and one CCl<sub>4</sub> control group. On the 30<sup>th</sup> day, the rats were killed and its liver examined with Haematoxyllin eosin staining. Histopathological changes were graded based on the degree of steatosis, hydropic degeneration, and inflammation. Data were analyzed with ANOVA and LSD post hoc (p≤0.05) using SPSS version 13.0</p> <p><strong>Results:</strong> The results showed improvement between the three treatment groups and the CCl<sub>4 </sub>control group about the degree of hydropic degeneration and inflammation (P ≤ 0.05). However, there were significant increased of steatosis 50 and 100 mg/kg bw treatment groups, before its significantly decrease at 200 mg/kg bw treatment group (2.5 g/kg).</p> <p><strong>Conclusions</strong>: <em>Fagraea racemosa</em> leaves methanolic extract could protect liver from free radicals generated by CCl4. The result indicated that <em>Fagraea racemosa</em> has promising quality to be explored as antioxidant supplement. <strong><em>(</em></strong><strong><em>Health Science Indones 2011;2:46-51)</em></strong></p> |
| first_indexed | 2024-12-21T19:47:59Z |
| format | Article |
| id | doaj.art-0d46d31585294c968de96caa4b3b2f68 |
| institution | Directory Open Access Journal |
| issn | 2087-7021 2338-3437 |
| language | English |
| last_indexed | 2024-12-21T19:47:59Z |
| publishDate | 2012-07-01 |
| publisher | Badan Penelitian dan Pengembangan Kesehatan |
| record_format | Article |
| series | Health Science Journal of Indonesia |
| spelling | doaj.art-0d46d31585294c968de96caa4b3b2f682022-12-21T18:52:16ZengBadan Penelitian dan Pengembangan KesehatanHealth Science Journal of Indonesia2087-70212338-34372012-07-0121 Apr465166Fagraea racemosa leaf extract inhibits oxidative stress-induced liver damage in Wistar ratsEva Rachmi0Yudanti Riastiti1Laboratory of Anatomy and Histology, Faculty of Medicine, Universitas MulawarmanLaboratory of Anatomy and Histology, Faculty of Medicine, Universitas Mulawarman<p><strong><em>Latar belakang</em></strong><em>: Kemampuan hati mengatasi stres oksidatif dapat ditingkatkan dengan konsumsi antioksidan eksogen yang berasal dari alam.</em><em> Penelitian ini ditujukan untuk mempelajari kemampuan hepatoprotektif dari ekstrak metanol daun Fagraea racemosa, dengan menggunakan CCL<sub>4</sub> sebagai model sumber radikal bebas. </em></p> <p><strong><em>Metode</em></strong><em>: </em><em>Tiga kelompok perlakuan tikus Wistar (enam ekor per kelompok), masing-masing diberi</em><em> dosis ekstrak</em><em> berturut-turut 50,</em><em> </em><em>100, 200 mg/kg bb per oral, sekali perhari selama 30 hari. CCl<sub>4</sub> diinjeksikan intraperitoneal kepada ketiga kelompok , dua kali per minggu (1,5 ml/kg bb). Sebagai pembanding, digunakan dua kelompok kontrol, yaitu kontrol normal dan kontrol CCl<sub>4</sub>. Pada hari ke-30, tikus dibunuh dan hati diwarnai dengan hematoksilin-eosin. Perubahan histopatologi ditentukan berdasar derajat steatosis, degenerasi hidropik, dan inflamasi. Data dianalisis dengan Anova dan uji post hoc LSD (p≤0.05) menggunakan SPSS versi 13.0</em></p> <p><strong><em>Hasil:</em></strong><em> Hasil menunjukkan perbaikan derajat degenerasi hidropik dan inflamasi (p≤0,05) pada ketiga kelompok perlakuan bila dibanding dengan kelompok kontrol CCl<sub>4. </sub>Tetapi, derajat steatosis meningkat pada kelompok perlakuan dosis 50 dan 100 mg/kg bb, dan kemudian menurun secara bermakna pada perlakuan 200 mg/kg bb. </em></p> <p><strong><em>Kesimpulan :</em></strong><em> Ekstrak methanol daun </em><em>Fagraea racemosa mampu melindungi hati dari radikal bebas yang dihasilkan dari CCl<sub>4</sub>. Hasil ini mengindikasikan bahwa Fagraea racemosa menjanjikan untuk dikembangkan sebagai suplemen antioksidan. </em><strong><em>(</em></strong><strong><em>Health Science Indones 2011;2:46-51)</em></strong><em></em></p> <p> </p> <p><strong>Abstract </strong></p> <p><strong>Background</strong>: The ability of the liver in dealing with oxidative stress can be enhanced by consumption of exogenous antioxidants derived from nature. This study aimed to explore the hepatoprotective ability of <em>Fagraea racemosa</em> leaves methanolic extract against CCl<sub>4</sub> exposure as a model of free radicals source.</p> <p><strong>Methods</strong>: Three different doses (50, 100, 200 mg/kg bw) were administered orally to three treatment groups of Wistar rats (six Wistar rats each), respectively, once per day for 30 days. CCl<sub>4</sub> injected intraperitoneally to those three groups, twice a week (1,5 ml/kg bw). Two control groups were provided that were one normal control group and one CCl<sub>4</sub> control group. On the 30<sup>th</sup> day, the rats were killed and its liver examined with Haematoxyllin eosin staining. Histopathological changes were graded based on the degree of steatosis, hydropic degeneration, and inflammation. Data were analyzed with ANOVA and LSD post hoc (p≤0.05) using SPSS version 13.0</p> <p><strong>Results:</strong> The results showed improvement between the three treatment groups and the CCl<sub>4 </sub>control group about the degree of hydropic degeneration and inflammation (P ≤ 0.05). However, there were significant increased of steatosis 50 and 100 mg/kg bw treatment groups, before its significantly decrease at 200 mg/kg bw treatment group (2.5 g/kg).</p> <p><strong>Conclusions</strong>: <em>Fagraea racemosa</em> leaves methanolic extract could protect liver from free radicals generated by CCl4. The result indicated that <em>Fagraea racemosa</em> has promising quality to be explored as antioxidant supplement. <strong><em>(</em></strong><strong><em>Health Science Indones 2011;2:46-51)</em></strong></p>http://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/63methanolic extract, Fagraea racemosa, hepatoprotective |
| spellingShingle | Eva Rachmi Yudanti Riastiti Fagraea racemosa leaf extract inhibits oxidative stress-induced liver damage in Wistar rats Health Science Journal of Indonesia methanolic extract, Fagraea racemosa, hepatoprotective |
| title | Fagraea racemosa leaf extract inhibits oxidative stress-induced liver damage in Wistar rats |
| title_full | Fagraea racemosa leaf extract inhibits oxidative stress-induced liver damage in Wistar rats |
| title_fullStr | Fagraea racemosa leaf extract inhibits oxidative stress-induced liver damage in Wistar rats |
| title_full_unstemmed | Fagraea racemosa leaf extract inhibits oxidative stress-induced liver damage in Wistar rats |
| title_short | Fagraea racemosa leaf extract inhibits oxidative stress-induced liver damage in Wistar rats |
| title_sort | fagraea racemosa leaf extract inhibits oxidative stress induced liver damage in wistar rats |
| topic | methanolic extract, Fagraea racemosa, hepatoprotective |
| url | http://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/63 |
| work_keys_str_mv | AT evarachmi fagraearacemosaleafextractinhibitsoxidativestressinducedliverdamageinwistarrats AT yudantiriastiti fagraearacemosaleafextractinhibitsoxidativestressinducedliverdamageinwistarrats |