Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato
High temperature stress is one of the major environmental factors that affect the growth and development of plants. Although WRKY transcription factors play a critical role in stress responses, there are few studies on the regulation of heat stress by WRKY transcription factors, especially in tomato...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-03-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014122001443 |
| _version_ | 1797271299015311360 |
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| author | Ying Wang Wenxian Gai Liangdan Yuan Lele Shang Fangman Li Zhao Gong Pingfei Ge Yaru Wang Jinbao Tao Xingyu Zhang Haiqiang Dong Yuyang Zhang |
| author_facet | Ying Wang Wenxian Gai Liangdan Yuan Lele Shang Fangman Li Zhao Gong Pingfei Ge Yaru Wang Jinbao Tao Xingyu Zhang Haiqiang Dong Yuyang Zhang |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | High temperature stress is one of the major environmental factors that affect the growth and development of plants. Although WRKY transcription factors play a critical role in stress responses, there are few studies on the regulation of heat stress by WRKY transcription factors, especially in tomato. Here, we identified a group I WRKY transcription factor, SlWRKY3, involved in thermotolerance in tomato. First, SlWRKY3 was induced and upregulated under heat stress. Accordingly, overexpression of SlWRKY3 led to an increase, whereas knock-out of SlWRKY3 resulted in decreased tolerance to heat stress. Overexpression of SlWRKY3 accumulated less reactive oxygen species (ROS), whereas knock-out of SlWRKY3 accumulated more ROS under heat stress. This indicated that SlWRKY3 positively regulates heat stress in tomato. In addition, SlWRKY3 activated the expression of a range of abiotic stress-responsive genes involved in ROS scavenging, such as a SlGRXS1 gene cluster. Further analysis showed that SlWRKY3 can bind to the promoters of the SlGRXS1 gene cluster and activate their expression. Collectively, these results imply that SlWRKY3 is a positive regulator of thermotolerance through direct binding to the promoters of the SlGRXS1 gene cluster and activating their expression and ROS scavenging. |
| first_indexed | 2024-03-07T14:00:43Z |
| format | Article |
| id | doaj.art-0d47009e7d2740ae94ee5485d02bfd2d |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-03-07T14:00:43Z |
| publishDate | 2024-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-0d47009e7d2740ae94ee5485d02bfd2d2024-03-07T05:28:44ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412024-03-01102515531Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomatoYing Wang0Wenxian Gai1Liangdan Yuan2Lele Shang3Fangman Li4Zhao Gong5Pingfei Ge6Yaru Wang7Jinbao Tao8Xingyu Zhang9Haiqiang Dong10Yuyang Zhang11National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China; Corresponding author. Tel.: +86 27 87282010.High temperature stress is one of the major environmental factors that affect the growth and development of plants. Although WRKY transcription factors play a critical role in stress responses, there are few studies on the regulation of heat stress by WRKY transcription factors, especially in tomato. Here, we identified a group I WRKY transcription factor, SlWRKY3, involved in thermotolerance in tomato. First, SlWRKY3 was induced and upregulated under heat stress. Accordingly, overexpression of SlWRKY3 led to an increase, whereas knock-out of SlWRKY3 resulted in decreased tolerance to heat stress. Overexpression of SlWRKY3 accumulated less reactive oxygen species (ROS), whereas knock-out of SlWRKY3 accumulated more ROS under heat stress. This indicated that SlWRKY3 positively regulates heat stress in tomato. In addition, SlWRKY3 activated the expression of a range of abiotic stress-responsive genes involved in ROS scavenging, such as a SlGRXS1 gene cluster. Further analysis showed that SlWRKY3 can bind to the promoters of the SlGRXS1 gene cluster and activate their expression. Collectively, these results imply that SlWRKY3 is a positive regulator of thermotolerance through direct binding to the promoters of the SlGRXS1 gene cluster and activating their expression and ROS scavenging.http://www.sciencedirect.com/science/article/pii/S2468014122001443TomatoWRKY transcription factorSlWRKY3ThermotoleranceSlGRXS1Gene cluster |
| spellingShingle | Ying Wang Wenxian Gai Liangdan Yuan Lele Shang Fangman Li Zhao Gong Pingfei Ge Yaru Wang Jinbao Tao Xingyu Zhang Haiqiang Dong Yuyang Zhang Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato Horticultural Plant Journal Tomato WRKY transcription factor SlWRKY3 Thermotolerance SlGRXS1 Gene cluster |
| title | Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato |
| title_full | Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato |
| title_fullStr | Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato |
| title_full_unstemmed | Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato |
| title_short | Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato |
| title_sort | heat inducible slwrky3 confers thermotolerance by activating the slgrxs1 gene cluster in tomato |
| topic | Tomato WRKY transcription factor SlWRKY3 Thermotolerance SlGRXS1 Gene cluster |
| url | http://www.sciencedirect.com/science/article/pii/S2468014122001443 |
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