Bacterial Community Structure in Anaerobic Digesters of a Full Scale Municipal Wastewater Treatment Plant ‒ Case Study of Dubai, United Arab Emirates
A highly complex microbial community involved in anaerobic sludge digesters plays vital roles in sludge treatment. The data on microbial ecology is important to accomplish efficient operation of the anaerobic digesters. This study is aimed at monitoring the bacterial community of three full-scale an...
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| Format: | Article |
| Language: | English |
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SDEWES Centre
2019-09-01
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| Series: | Journal of Sustainable Development of Energy, Water and Environment Systems |
| Subjects: | |
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http://www.sdewes.org/jsdewes/pid6.0222
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| _version_ | 1811240500065206272 |
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| author | Munawwar A. Khan Noorun N. Ashar Arpitha G. Ganesh Naushad Rais Sultan M. Faheem Shams T. Khan |
| author_facet | Munawwar A. Khan Noorun N. Ashar Arpitha G. Ganesh Naushad Rais Sultan M. Faheem Shams T. Khan |
| author_sort | Munawwar A. Khan |
| collection | DOAJ |
| description | A highly complex microbial community involved in anaerobic sludge digesters plays vital roles in sludge treatment. The data on microbial ecology is important to accomplish efficient operation of the anaerobic digesters. This study is aimed at monitoring the bacterial community of three full-scale anaerobic digesters of a full-scale municipal wastewater treatment Plant in Dubai, United Arab Emirates. Fluorescent in-situ hybridization technique was applied to identify the bacterial groups and quantitative polymerase chain reaction to compare the richness of bacterial and archaeal domain. Results of the fluorescent in-situ hybridization technique analysis showed that the phylum proteobacteriawas most abundant followed by cytophage-Flavobacterium group of Bacteroides, Firmicutes and Actinobacteria. Among proteobacterial subclass Delta- and Alpha- were dominating than Gamma- and Beta-proteobacteria. The genus Desulfobacter and Desulfobacterium were the dominant groups hybridizing 70-76% of total 4’, 6’‒ diamidino – 2 phenylindole stained cells. The quantitative polymerase chain reaction results showed that Bacterial domain was dominating in all three digesters compared to the Archaeal domain. |
| first_indexed | 2024-04-12T13:21:23Z |
| format | Article |
| id | doaj.art-0d47072ac66b481f94af2d9e66700d3c |
| institution | Directory Open Access Journal |
| issn | 1848-9257 |
| language | English |
| last_indexed | 2024-04-12T13:21:23Z |
| publishDate | 2019-09-01 |
| publisher | SDEWES Centre |
| record_format | Article |
| series | Journal of Sustainable Development of Energy, Water and Environment Systems |
| spelling | doaj.art-0d47072ac66b481f94af2d9e66700d3c2022-12-22T03:31:27ZengSDEWES CentreJournal of Sustainable Development of Energy, Water and Environment Systems1848-92572019-09-017338539810.13044/j.sdewes.d6.022200222Bacterial Community Structure in Anaerobic Digesters of a Full Scale Municipal Wastewater Treatment Plant ‒ Case Study of Dubai, United Arab EmiratesMunawwar A. Khan0Noorun N. Ashar1Arpitha G. Ganesh2Naushad Rais3Sultan M. Faheem4Shams T. Khan5 Department of Life and Environmental Sciences, College of Natural and Health Sciences, Zayed University, P.O. Box: 19282, Dubai, United Arab Emirates School of Life Sciences, Manipal University, Dubai International Academic City, P.O. Box: 345050, Dubai, United Arab Emirates Department of Life and Environmental Sciences, College of Natural and Health Sciences, Zayed University, P.O. Box: 19282, Dubai, United Arab Emirates School of Life Sciences, Manipal University, Dubai International Academic City, P.O. Box: 345050, Dubai, United Arab Emirates School of Life Sciences, Manipal University, Dubai International Academic City, P.O. Box: 345050, Dubai, United Arab Emirates Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, Uttar Pradesh 2002002, India A highly complex microbial community involved in anaerobic sludge digesters plays vital roles in sludge treatment. The data on microbial ecology is important to accomplish efficient operation of the anaerobic digesters. This study is aimed at monitoring the bacterial community of three full-scale anaerobic digesters of a full-scale municipal wastewater treatment Plant in Dubai, United Arab Emirates. Fluorescent in-situ hybridization technique was applied to identify the bacterial groups and quantitative polymerase chain reaction to compare the richness of bacterial and archaeal domain. Results of the fluorescent in-situ hybridization technique analysis showed that the phylum proteobacteriawas most abundant followed by cytophage-Flavobacterium group of Bacteroides, Firmicutes and Actinobacteria. Among proteobacterial subclass Delta- and Alpha- were dominating than Gamma- and Beta-proteobacteria. The genus Desulfobacter and Desulfobacterium were the dominant groups hybridizing 70-76% of total 4’, 6’‒ diamidino – 2 phenylindole stained cells. The quantitative polymerase chain reaction results showed that Bacterial domain was dominating in all three digesters compared to the Archaeal domain. http://www.sdewes.org/jsdewes/pid6.0222 Anaerobic digesterBacterial communityFluorescence in situ hybridizationOligonucleotide probesQuantitative polymerase chain reaction. |
| spellingShingle | Munawwar A. Khan Noorun N. Ashar Arpitha G. Ganesh Naushad Rais Sultan M. Faheem Shams T. Khan Bacterial Community Structure in Anaerobic Digesters of a Full Scale Municipal Wastewater Treatment Plant ‒ Case Study of Dubai, United Arab Emirates Journal of Sustainable Development of Energy, Water and Environment Systems Anaerobic digester Bacterial community Fluorescence in situ hybridization Oligonucleotide probes Quantitative polymerase chain reaction. |
| title | Bacterial Community Structure in Anaerobic Digesters of a Full Scale Municipal Wastewater Treatment Plant ‒ Case Study of Dubai, United Arab Emirates |
| title_full | Bacterial Community Structure in Anaerobic Digesters of a Full Scale Municipal Wastewater Treatment Plant ‒ Case Study of Dubai, United Arab Emirates |
| title_fullStr | Bacterial Community Structure in Anaerobic Digesters of a Full Scale Municipal Wastewater Treatment Plant ‒ Case Study of Dubai, United Arab Emirates |
| title_full_unstemmed | Bacterial Community Structure in Anaerobic Digesters of a Full Scale Municipal Wastewater Treatment Plant ‒ Case Study of Dubai, United Arab Emirates |
| title_short | Bacterial Community Structure in Anaerobic Digesters of a Full Scale Municipal Wastewater Treatment Plant ‒ Case Study of Dubai, United Arab Emirates |
| title_sort | bacterial community structure in anaerobic digesters of a full scale municipal wastewater treatment plant case study of dubai united arab emirates |
| topic | Anaerobic digester Bacterial community Fluorescence in situ hybridization Oligonucleotide probes Quantitative polymerase chain reaction. |
| url |
http://www.sdewes.org/jsdewes/pid6.0222
|
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