Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration
In this work, a poly(L-lactide-<i>co</i>-glycolide) (PLGA)-based composite was enriched with one of the following sol-gel bioactive glasses (SBG) at 50 wt.%: A1—40 mol% SiO<sub>2</sub>, 60 mol% CaO, CaO/SiO<sub>2</sub> ratio of 1.50; S1—80 mol% SiO<sub>2<...
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/5/2598 |
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| author | Krzysztof Łukowicz Barbara Zagrajczuk Karolina Truchan Łukasz Niedzwiedzki Katarzyna Cholewa-Kowalska Anna M. Osyczka |
| author_facet | Krzysztof Łukowicz Barbara Zagrajczuk Karolina Truchan Łukasz Niedzwiedzki Katarzyna Cholewa-Kowalska Anna M. Osyczka |
| author_sort | Krzysztof Łukowicz |
| collection | DOAJ |
| description | In this work, a poly(L-lactide-<i>co</i>-glycolide) (PLGA)-based composite was enriched with one of the following sol-gel bioactive glasses (SBG) at 50 wt.%: A1—40 mol% SiO<sub>2</sub>, 60 mol% CaO, CaO/SiO<sub>2</sub> ratio of 1.50; S1—80 mol% SiO<sub>2</sub>, 20 mol% CaO, CaO/SiO<sub>2</sub> ratio of 0.25; A2—40 mol% SiO<sub>2</sub>, 54 mol% CaO, 6 mol% P<sub>2</sub>O<sub>5</sub>, CaO/SiO<sub>2</sub> ratio of 1.35; S2—80 mol% SiO<sub>2</sub>,16 mol% CaO, 4 mol% P<sub>2</sub>O<sub>5</sub>, CaO/SiO<sub>2</sub> ratio of 0.20. The composites and PLGA control sheets were then soaked for 24 h in culture media, and the obtained condition media (CM) were used to treat human bone marrow stromal cells (hBMSCs) for 72 h. All CMs from the composites increased ERK 1/2 activity vs. the control PLGA CM. However, expressions of cell migration-related c-Fos, osteopontin, matrix metalloproteinase-2, C-X-C chemokine receptor type 4, vascular endothelial growth factor, and bone morphogenetic protein 2 were significantly increased only in cells treated with the CM from the A1/PLGA composite. This CM also significantly increased the rate of human BMSC migration but did not affect cell metabolic activity. These results indicate important biological markers that are upregulated by products released from the bioactive composites of a specific chemical composition, which may eventually prompt osteoprogenitor cells to colonize the bioactive material and accelerate the process of tissue regeneration. |
| first_indexed | 2024-03-09T20:37:33Z |
| format | Article |
| id | doaj.art-0d49780bea8040d9963cc9355f55b6c4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:37:33Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0d49780bea8040d9963cc9355f55b6c42023-11-23T23:06:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01235259810.3390/ijms23052598Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell MigrationKrzysztof Łukowicz0Barbara Zagrajczuk1Karolina Truchan2Łukasz Niedzwiedzki3Katarzyna Cholewa-Kowalska4Anna M. Osyczka5Department Biology and Cell Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, PolandDepartment of Glass Technology and Amorphous Coatings, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Mickiewicza Ave. 30, 30-059 Krakow, PolandDepartment Biology and Cell Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, PolandDepartment of Orthopedics and Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, Kopernika 19e, 31-501 Krakow, PolandDepartment of Glass Technology and Amorphous Coatings, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Mickiewicza Ave. 30, 30-059 Krakow, PolandDepartment Biology and Cell Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, PolandIn this work, a poly(L-lactide-<i>co</i>-glycolide) (PLGA)-based composite was enriched with one of the following sol-gel bioactive glasses (SBG) at 50 wt.%: A1—40 mol% SiO<sub>2</sub>, 60 mol% CaO, CaO/SiO<sub>2</sub> ratio of 1.50; S1—80 mol% SiO<sub>2</sub>, 20 mol% CaO, CaO/SiO<sub>2</sub> ratio of 0.25; A2—40 mol% SiO<sub>2</sub>, 54 mol% CaO, 6 mol% P<sub>2</sub>O<sub>5</sub>, CaO/SiO<sub>2</sub> ratio of 1.35; S2—80 mol% SiO<sub>2</sub>,16 mol% CaO, 4 mol% P<sub>2</sub>O<sub>5</sub>, CaO/SiO<sub>2</sub> ratio of 0.20. The composites and PLGA control sheets were then soaked for 24 h in culture media, and the obtained condition media (CM) were used to treat human bone marrow stromal cells (hBMSCs) for 72 h. All CMs from the composites increased ERK 1/2 activity vs. the control PLGA CM. However, expressions of cell migration-related c-Fos, osteopontin, matrix metalloproteinase-2, C-X-C chemokine receptor type 4, vascular endothelial growth factor, and bone morphogenetic protein 2 were significantly increased only in cells treated with the CM from the A1/PLGA composite. This CM also significantly increased the rate of human BMSC migration but did not affect cell metabolic activity. These results indicate important biological markers that are upregulated by products released from the bioactive composites of a specific chemical composition, which may eventually prompt osteoprogenitor cells to colonize the bioactive material and accelerate the process of tissue regeneration.https://www.mdpi.com/1422-0067/23/5/2598compositesstem cellscell migration |
| spellingShingle | Krzysztof Łukowicz Barbara Zagrajczuk Karolina Truchan Łukasz Niedzwiedzki Katarzyna Cholewa-Kowalska Anna M. Osyczka Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration International Journal of Molecular Sciences composites stem cells cell migration |
| title | Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration |
| title_full | Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration |
| title_fullStr | Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration |
| title_full_unstemmed | Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration |
| title_short | Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration |
| title_sort | chemical compounds released from specific osteoinductive bioactive materials stimulate human bone marrow mesenchymal stem cell migration |
| topic | composites stem cells cell migration |
| url | https://www.mdpi.com/1422-0067/23/5/2598 |
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