Long Noncoding RNA GAS5 Contained in Exosomes Derived from Human Adipose Stem Cells Promotes Repair and Modulates Inflammation in a Chronic Dermal Wound Healing Model
Chronic recalcitrant wounds result from delayed or slowed healing processes. Underlying inflammation is a substantial risk factor for impaired dermal wound healing and often leads to chronic wound-related sequelae. Human adipose stem cells (hASCs) have shown tremendous potential in regenerative medi...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/3/426 |
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| author | Rekha S. Patel Sabrina Impreso Ashley Lui Gitanjali Vidyarthi Paul Albear Niketa A. Patel |
| author_facet | Rekha S. Patel Sabrina Impreso Ashley Lui Gitanjali Vidyarthi Paul Albear Niketa A. Patel |
| author_sort | Rekha S. Patel |
| collection | DOAJ |
| description | Chronic recalcitrant wounds result from delayed or slowed healing processes. Underlying inflammation is a substantial risk factor for impaired dermal wound healing and often leads to chronic wound-related sequelae. Human adipose stem cells (hASCs) have shown tremendous potential in regenerative medicine. The goal of this project was to improve the outcome of chronic wounds by harvesting the exosomes from hASCs for therapeutic intervention. The results demonstrate that long noncoding RNA GAS5 is highly enriched in hASC exosomes and, further, that GAS5 is central to promoting wound repair in vitro. To evaluate the outcome of wound healing in a chronic low-grade inflammatory environment, lipopolysaccharide-treated HDF cells were evaluated for their response to hASC exosome treatment. Ingenuity pathway analysis identified inflammation pathways and genes affected by exosomes in a GAS5-dependent manner. Using siRNA to deplete GAS5 in HDF, the results demonstrated that Toll-like receptor 7 (TLR7) expression levels were regulated by GAS5. Importantly, the results demonstrate that GAS5 regulates inflammatory pathway genes in a chronic inflammation environment. The results presented here demonstrate that hASC exosomes are a viable therapeutic that accelerate the healing of chronic recalcitrant wounds. |
| first_indexed | 2024-03-09T13:49:44Z |
| format | Article |
| id | doaj.art-0d4b202db6e1462a8ac36ab94358ff24 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T13:49:44Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-0d4b202db6e1462a8ac36ab94358ff242023-11-30T20:52:02ZengMDPI AGBiology2079-77372022-03-0111342610.3390/biology11030426Long Noncoding RNA GAS5 Contained in Exosomes Derived from Human Adipose Stem Cells Promotes Repair and Modulates Inflammation in a Chronic Dermal Wound Healing ModelRekha S. Patel0Sabrina Impreso1Ashley Lui2Gitanjali Vidyarthi3Paul Albear4Niketa A. Patel5James A. Haley Veteran’s Hospital, 13000 Bruce B Downs Blvd, Tampa, FL 33612, USAJames A. Haley Veteran’s Hospital, 13000 Bruce B Downs Blvd, Tampa, FL 33612, USADepartment of Molecular Medicine, University of South Florida, Tampa, FL 33612, USAJames A. Haley Veteran’s Hospital, 13000 Bruce B Downs Blvd, Tampa, FL 33612, USAJames A. Haley Veteran’s Hospital, 13000 Bruce B Downs Blvd, Tampa, FL 33612, USAJames A. Haley Veteran’s Hospital, 13000 Bruce B Downs Blvd, Tampa, FL 33612, USAChronic recalcitrant wounds result from delayed or slowed healing processes. Underlying inflammation is a substantial risk factor for impaired dermal wound healing and often leads to chronic wound-related sequelae. Human adipose stem cells (hASCs) have shown tremendous potential in regenerative medicine. The goal of this project was to improve the outcome of chronic wounds by harvesting the exosomes from hASCs for therapeutic intervention. The results demonstrate that long noncoding RNA GAS5 is highly enriched in hASC exosomes and, further, that GAS5 is central to promoting wound repair in vitro. To evaluate the outcome of wound healing in a chronic low-grade inflammatory environment, lipopolysaccharide-treated HDF cells were evaluated for their response to hASC exosome treatment. Ingenuity pathway analysis identified inflammation pathways and genes affected by exosomes in a GAS5-dependent manner. Using siRNA to deplete GAS5 in HDF, the results demonstrated that Toll-like receptor 7 (TLR7) expression levels were regulated by GAS5. Importantly, the results demonstrate that GAS5 regulates inflammatory pathway genes in a chronic inflammation environment. The results presented here demonstrate that hASC exosomes are a viable therapeutic that accelerate the healing of chronic recalcitrant wounds.https://www.mdpi.com/2079-7737/11/3/426long noncoding RNA (lncRNA)GAS5exosomesinflammationwound healinghuman adipose stem cells |
| spellingShingle | Rekha S. Patel Sabrina Impreso Ashley Lui Gitanjali Vidyarthi Paul Albear Niketa A. Patel Long Noncoding RNA GAS5 Contained in Exosomes Derived from Human Adipose Stem Cells Promotes Repair and Modulates Inflammation in a Chronic Dermal Wound Healing Model Biology long noncoding RNA (lncRNA) GAS5 exosomes inflammation wound healing human adipose stem cells |
| title | Long Noncoding RNA GAS5 Contained in Exosomes Derived from Human Adipose Stem Cells Promotes Repair and Modulates Inflammation in a Chronic Dermal Wound Healing Model |
| title_full | Long Noncoding RNA GAS5 Contained in Exosomes Derived from Human Adipose Stem Cells Promotes Repair and Modulates Inflammation in a Chronic Dermal Wound Healing Model |
| title_fullStr | Long Noncoding RNA GAS5 Contained in Exosomes Derived from Human Adipose Stem Cells Promotes Repair and Modulates Inflammation in a Chronic Dermal Wound Healing Model |
| title_full_unstemmed | Long Noncoding RNA GAS5 Contained in Exosomes Derived from Human Adipose Stem Cells Promotes Repair and Modulates Inflammation in a Chronic Dermal Wound Healing Model |
| title_short | Long Noncoding RNA GAS5 Contained in Exosomes Derived from Human Adipose Stem Cells Promotes Repair and Modulates Inflammation in a Chronic Dermal Wound Healing Model |
| title_sort | long noncoding rna gas5 contained in exosomes derived from human adipose stem cells promotes repair and modulates inflammation in a chronic dermal wound healing model |
| topic | long noncoding RNA (lncRNA) GAS5 exosomes inflammation wound healing human adipose stem cells |
| url | https://www.mdpi.com/2079-7737/11/3/426 |
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