Radiotherapy for the treatment of pulmonary hydatidosis in sheep
Hydatidosis is an endemic disease causing a severe threat to public health. Drugs and surgery have been utilized for treatment, but their efficiency is not adequate. Therefore, new methods are required for treating such diseases. In this study, we attempt to evaluate the efficiency of radiotherapy f...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2021-12-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2021-0139 |
| _version_ | 1828383463943700480 |
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| author | Zhang Yuefen Lu Pengfei Qi Hongzhi Wu Ge Mao Rui Bao Yongxing |
| author_facet | Zhang Yuefen Lu Pengfei Qi Hongzhi Wu Ge Mao Rui Bao Yongxing |
| author_sort | Zhang Yuefen |
| collection | DOAJ |
| description | Hydatidosis is an endemic disease causing a severe threat to public health. Drugs and surgery have been utilized for treatment, but their efficiency is not adequate. Therefore, new methods are required for treating such diseases. In this study, we attempt to evaluate the efficiency of radiotherapy for hydatidosis in sheep. The sheep naturally infected with pulmonary hydatid were randomly divided into four groups, including the control group subjected to no irradiation and the other three groups subjected to 30, 45, and 60 Gy irradiation, respectively. Gene expression of caspase-3 and gadd45a and protein expression of BCL-2 and BAX in the lung tissues were evaluated after treatment. Our data showed that the irradiation with a dose of 30, 45, and 60 Gy significantly induced the expression of caspase-3 and gadd45a. Immunohistochemical staining showed that the BCL-2 protein was downregulated after exposure to 45 Gy of irradiation, whereas the BAX expression was downregulated after irradiation at a dose of 45 and 60 Gy, respectively. On this basis, we speculated that 45 Gy might be a safe and effective dose for treating pulmonary hydatidosis in sheep, which induced lower expression of caspase-3 and gadd45a in the cyst and a downregulation of BCL-2 and BAX in the adjacent lung tissues. |
| first_indexed | 2024-12-10T04:49:37Z |
| format | Article |
| id | doaj.art-0d4b3204f42c4b82839fc705c5d312ec |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-10T04:49:37Z |
| publishDate | 2021-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-0d4b3204f42c4b82839fc705c5d312ec2022-12-22T02:01:39ZengDe GruyterOpen Life Sciences2391-54122021-12-011611405141110.1515/biol-2021-0139Radiotherapy for the treatment of pulmonary hydatidosis in sheepZhang Yuefen0Lu Pengfei1Qi Hongzhi2Wu Ge3Mao Rui4Bao Yongxing5Department of Radiation Oncology, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi 830054, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi 830054, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi 830054, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi 830054, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi 830054, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi 830054, ChinaHydatidosis is an endemic disease causing a severe threat to public health. Drugs and surgery have been utilized for treatment, but their efficiency is not adequate. Therefore, new methods are required for treating such diseases. In this study, we attempt to evaluate the efficiency of radiotherapy for hydatidosis in sheep. The sheep naturally infected with pulmonary hydatid were randomly divided into four groups, including the control group subjected to no irradiation and the other three groups subjected to 30, 45, and 60 Gy irradiation, respectively. Gene expression of caspase-3 and gadd45a and protein expression of BCL-2 and BAX in the lung tissues were evaluated after treatment. Our data showed that the irradiation with a dose of 30, 45, and 60 Gy significantly induced the expression of caspase-3 and gadd45a. Immunohistochemical staining showed that the BCL-2 protein was downregulated after exposure to 45 Gy of irradiation, whereas the BAX expression was downregulated after irradiation at a dose of 45 and 60 Gy, respectively. On this basis, we speculated that 45 Gy might be a safe and effective dose for treating pulmonary hydatidosis in sheep, which induced lower expression of caspase-3 and gadd45a in the cyst and a downregulation of BCL-2 and BAX in the adjacent lung tissues.https://doi.org/10.1515/biol-2021-0139radiotherapypulmonary hydatidosissheepapoptosisirradiation |
| spellingShingle | Zhang Yuefen Lu Pengfei Qi Hongzhi Wu Ge Mao Rui Bao Yongxing Radiotherapy for the treatment of pulmonary hydatidosis in sheep Open Life Sciences radiotherapy pulmonary hydatidosis sheep apoptosis irradiation |
| title | Radiotherapy for the treatment of pulmonary hydatidosis in sheep |
| title_full | Radiotherapy for the treatment of pulmonary hydatidosis in sheep |
| title_fullStr | Radiotherapy for the treatment of pulmonary hydatidosis in sheep |
| title_full_unstemmed | Radiotherapy for the treatment of pulmonary hydatidosis in sheep |
| title_short | Radiotherapy for the treatment of pulmonary hydatidosis in sheep |
| title_sort | radiotherapy for the treatment of pulmonary hydatidosis in sheep |
| topic | radiotherapy pulmonary hydatidosis sheep apoptosis irradiation |
| url | https://doi.org/10.1515/biol-2021-0139 |
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