Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions.
Mitochondria are the primary organelles that consume oxygen and provide energy for cellular activities. To investigate the mitochondrial mechanisms underlying adaptation to extreme oxygen conditions, we generated Drosophila strains that could survive in low- or high-oxygen environments (LOF or HOF,...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3771901?pdf=render |
| _version_ | 1828771770712195072 |
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| author | Songyue Yin Jin Xue Haidan Sun Bo Wen Quanhui Wang Guy Perkins Huiwen W Zhao Mark H Ellisman Yu-hsin Hsiao Liang Yin Yingying Xie Guixue Hou Jin Zi Liang Lin Gabriel G Haddad Dan Zhou Siqi Liu |
| author_facet | Songyue Yin Jin Xue Haidan Sun Bo Wen Quanhui Wang Guy Perkins Huiwen W Zhao Mark H Ellisman Yu-hsin Hsiao Liang Yin Yingying Xie Guixue Hou Jin Zi Liang Lin Gabriel G Haddad Dan Zhou Siqi Liu |
| author_sort | Songyue Yin |
| collection | DOAJ |
| description | Mitochondria are the primary organelles that consume oxygen and provide energy for cellular activities. To investigate the mitochondrial mechanisms underlying adaptation to extreme oxygen conditions, we generated Drosophila strains that could survive in low- or high-oxygen environments (LOF or HOF, respectively), examined their mitochondria at the ultrastructural level via transmission electron microscopy, studied the activity of their respiratory chain complexes, and quantitatively analyzed the protein abundance responses of the mitochondrial proteomes using Isobaric tag for relative and absolute quantitation (iTRAQ). A total of 718 proteins were identified with high confidence, and 55 and 75 mitochondrial proteins displayed significant differences in abundance in LOF and HOF, respectively, compared with the control flies. Importantly, these differentially expressed mitochondrial proteins are primarily involved in respiration, calcium regulation, the oxidative response, and mitochondrial protein translation. A correlation analysis of the changes in the levels of the mRNAs corresponding to differentially regulated mitochondrial proteins revealed two sets of proteins with different modes of regulation (transcriptional vs. post-transcriptional) in both LOF and HOF. We believe that these findings will not only enhance our understanding of the mechanisms underlying adaptation to extreme oxygen conditions in Drosophila but also provide a clue in studying human disease induced by altered oxygen tension in tissues and cells. |
| first_indexed | 2024-12-11T14:31:26Z |
| format | Article |
| id | doaj.art-0d4cf1c38b1e434ba1a12adca2592216 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T14:31:26Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0d4cf1c38b1e434ba1a12adca25922162022-12-22T01:02:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7401110.1371/journal.pone.0074011Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions.Songyue YinJin XueHaidan SunBo WenQuanhui WangGuy PerkinsHuiwen W ZhaoMark H EllismanYu-hsin HsiaoLiang YinYingying XieGuixue HouJin ZiLiang LinGabriel G HaddadDan ZhouSiqi LiuMitochondria are the primary organelles that consume oxygen and provide energy for cellular activities. To investigate the mitochondrial mechanisms underlying adaptation to extreme oxygen conditions, we generated Drosophila strains that could survive in low- or high-oxygen environments (LOF or HOF, respectively), examined their mitochondria at the ultrastructural level via transmission electron microscopy, studied the activity of their respiratory chain complexes, and quantitatively analyzed the protein abundance responses of the mitochondrial proteomes using Isobaric tag for relative and absolute quantitation (iTRAQ). A total of 718 proteins were identified with high confidence, and 55 and 75 mitochondrial proteins displayed significant differences in abundance in LOF and HOF, respectively, compared with the control flies. Importantly, these differentially expressed mitochondrial proteins are primarily involved in respiration, calcium regulation, the oxidative response, and mitochondrial protein translation. A correlation analysis of the changes in the levels of the mRNAs corresponding to differentially regulated mitochondrial proteins revealed two sets of proteins with different modes of regulation (transcriptional vs. post-transcriptional) in both LOF and HOF. We believe that these findings will not only enhance our understanding of the mechanisms underlying adaptation to extreme oxygen conditions in Drosophila but also provide a clue in studying human disease induced by altered oxygen tension in tissues and cells.http://europepmc.org/articles/PMC3771901?pdf=render |
| spellingShingle | Songyue Yin Jin Xue Haidan Sun Bo Wen Quanhui Wang Guy Perkins Huiwen W Zhao Mark H Ellisman Yu-hsin Hsiao Liang Yin Yingying Xie Guixue Hou Jin Zi Liang Lin Gabriel G Haddad Dan Zhou Siqi Liu Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions. PLoS ONE |
| title | Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions. |
| title_full | Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions. |
| title_fullStr | Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions. |
| title_full_unstemmed | Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions. |
| title_short | Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions. |
| title_sort | quantitative evaluation of the mitochondrial proteomes of drosophila melanogaster adapted to extreme oxygen conditions |
| url | http://europepmc.org/articles/PMC3771901?pdf=render |
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