HIF-1α Negatively Regulates Irisin Expression Which Involves in Muscle Atrophy Induced by Hypoxia
Exposure to high altitude environment leads to skeletal muscle atrophy. As a hormone secreted by skeletal muscles after exercise, irisin contributes to promoting muscle regeneration and ameliorating skeletal muscle atrophy, but its role in hypoxia-induced skeletal muscle atrophy is still unclear. Ou...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/2/887 |
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| author | Shiqiang Liu Pengyu Fu Kaiting Ning Rui Wang Baoqiang Yang Jiahui Chen Huiyun Xu |
| author_facet | Shiqiang Liu Pengyu Fu Kaiting Ning Rui Wang Baoqiang Yang Jiahui Chen Huiyun Xu |
| author_sort | Shiqiang Liu |
| collection | DOAJ |
| description | Exposure to high altitude environment leads to skeletal muscle atrophy. As a hormone secreted by skeletal muscles after exercise, irisin contributes to promoting muscle regeneration and ameliorating skeletal muscle atrophy, but its role in hypoxia-induced skeletal muscle atrophy is still unclear. Our results showed that 4 w of hypoxia exposure significantly reduced body weight and gastrocnemius muscle mass of mice, as well as grip strength and the duration time of treadmill exercise. Hypoxic treatment increased HIF-1α expression and decreased both the circulation level of irisin and its precursor protein FNDC5 expression in skeletal muscle. In in vitro, CoCl<sub>2</sub>-induced chemical hypoxia and 1% O<sub>2</sub> ambient hypoxia both reduced FNDC5, along with the increase in HIF-1α. Moreover, the decline in the area and diameter of myotubes caused by hypoxia were rescued by inhibiting HIF-1α via YC-1. Collectively, our research indicated that FNDC5/irisin was negatively regulated by HIF-1α and could participate in the regulation of muscle atrophy caused by hypoxia. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:17:42Z |
| publishDate | 2022-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0d4e6c6034bb41eb8f76a63161df886c2023-11-23T14:06:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-0123288710.3390/ijms23020887HIF-1α Negatively Regulates Irisin Expression Which Involves in Muscle Atrophy Induced by HypoxiaShiqiang Liu0Pengyu Fu1Kaiting Ning2Rui Wang3Baoqiang Yang4Jiahui Chen5Huiyun Xu6Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaDepartment of Physical Education, Northwestern Polytechnical University, Xi’an 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaExposure to high altitude environment leads to skeletal muscle atrophy. As a hormone secreted by skeletal muscles after exercise, irisin contributes to promoting muscle regeneration and ameliorating skeletal muscle atrophy, but its role in hypoxia-induced skeletal muscle atrophy is still unclear. Our results showed that 4 w of hypoxia exposure significantly reduced body weight and gastrocnemius muscle mass of mice, as well as grip strength and the duration time of treadmill exercise. Hypoxic treatment increased HIF-1α expression and decreased both the circulation level of irisin and its precursor protein FNDC5 expression in skeletal muscle. In in vitro, CoCl<sub>2</sub>-induced chemical hypoxia and 1% O<sub>2</sub> ambient hypoxia both reduced FNDC5, along with the increase in HIF-1α. Moreover, the decline in the area and diameter of myotubes caused by hypoxia were rescued by inhibiting HIF-1α via YC-1. Collectively, our research indicated that FNDC5/irisin was negatively regulated by HIF-1α and could participate in the regulation of muscle atrophy caused by hypoxia.https://www.mdpi.com/1422-0067/23/2/887hypoxiamuscle atrophyHIF-1αirisinFNDC5 |
| spellingShingle | Shiqiang Liu Pengyu Fu Kaiting Ning Rui Wang Baoqiang Yang Jiahui Chen Huiyun Xu HIF-1α Negatively Regulates Irisin Expression Which Involves in Muscle Atrophy Induced by Hypoxia International Journal of Molecular Sciences hypoxia muscle atrophy HIF-1α irisin FNDC5 |
| title | HIF-1α Negatively Regulates Irisin Expression Which Involves in Muscle Atrophy Induced by Hypoxia |
| title_full | HIF-1α Negatively Regulates Irisin Expression Which Involves in Muscle Atrophy Induced by Hypoxia |
| title_fullStr | HIF-1α Negatively Regulates Irisin Expression Which Involves in Muscle Atrophy Induced by Hypoxia |
| title_full_unstemmed | HIF-1α Negatively Regulates Irisin Expression Which Involves in Muscle Atrophy Induced by Hypoxia |
| title_short | HIF-1α Negatively Regulates Irisin Expression Which Involves in Muscle Atrophy Induced by Hypoxia |
| title_sort | hif 1α negatively regulates irisin expression which involves in muscle atrophy induced by hypoxia |
| topic | hypoxia muscle atrophy HIF-1α irisin FNDC5 |
| url | https://www.mdpi.com/1422-0067/23/2/887 |
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