Enzyme-Instructed Aggregation/Dispersion of Fluorophores for Near-Infrared Fluorescence Imaging In Vivo
Near-infrared (NIR) fluorescence is a noninvasive, highly sensitive, and high-resolution modality with great potential for in vivo imaging. Compared with “Always-On” probes, activatable NIR fluorescent probes with “Turn-Off/On” or “Ratiometric” fluorescent signals at target sites exhibit better sign...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/14/5360 |
| _version_ | 1797587982597750784 |
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| author | Zhipeng Zhang Peiyao Chen Yao Sun |
| author_facet | Zhipeng Zhang Peiyao Chen Yao Sun |
| author_sort | Zhipeng Zhang |
| collection | DOAJ |
| description | Near-infrared (NIR) fluorescence is a noninvasive, highly sensitive, and high-resolution modality with great potential for in vivo imaging. Compared with “Always-On” probes, activatable NIR fluorescent probes with “Turn-Off/On” or “Ratiometric” fluorescent signals at target sites exhibit better signal-to-noise ratio (SNR), wherein enzymes are one of the ideal triggers for probe activation, which play vital roles in a variety of biological processes. In this review, we provide an overview of enzyme-activatable NIR fluorescent probes and concentrate on the design strategies and sensing mechanisms. We focus on the aggregation/dispersion state of fluorophores after the interaction of probes and enzymes and finally discuss the current challenges and provide some perspective ideas for the construction of enzyme-activatable NIR fluorescent probes. |
| first_indexed | 2024-03-11T00:47:34Z |
| format | Article |
| id | doaj.art-0d4fa89b3dd9437bb3c5c2e4f40b3d10 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:47:34Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0d4fa89b3dd9437bb3c5c2e4f40b3d102023-11-18T20:40:59ZengMDPI AGMolecules1420-30492023-07-012814536010.3390/molecules28145360Enzyme-Instructed Aggregation/Dispersion of Fluorophores for Near-Infrared Fluorescence Imaging In VivoZhipeng Zhang0Peiyao Chen1Yao Sun2Xianning Medical College, Hubei University of Science & Technology, Xianning 437000, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Key Laboratory of Industrial Microbiology, School of Food and Biological Engineering, Hubei University of Technology, Wuhan 430068, ChinaNational Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan 430079, ChinaNear-infrared (NIR) fluorescence is a noninvasive, highly sensitive, and high-resolution modality with great potential for in vivo imaging. Compared with “Always-On” probes, activatable NIR fluorescent probes with “Turn-Off/On” or “Ratiometric” fluorescent signals at target sites exhibit better signal-to-noise ratio (SNR), wherein enzymes are one of the ideal triggers for probe activation, which play vital roles in a variety of biological processes. In this review, we provide an overview of enzyme-activatable NIR fluorescent probes and concentrate on the design strategies and sensing mechanisms. We focus on the aggregation/dispersion state of fluorophores after the interaction of probes and enzymes and finally discuss the current challenges and provide some perspective ideas for the construction of enzyme-activatable NIR fluorescent probes.https://www.mdpi.com/1420-3049/28/14/5360near-infrared fluorescenceenzymeactivatable probe |
| spellingShingle | Zhipeng Zhang Peiyao Chen Yao Sun Enzyme-Instructed Aggregation/Dispersion of Fluorophores for Near-Infrared Fluorescence Imaging In Vivo Molecules near-infrared fluorescence enzyme activatable probe |
| title | Enzyme-Instructed Aggregation/Dispersion of Fluorophores for Near-Infrared Fluorescence Imaging In Vivo |
| title_full | Enzyme-Instructed Aggregation/Dispersion of Fluorophores for Near-Infrared Fluorescence Imaging In Vivo |
| title_fullStr | Enzyme-Instructed Aggregation/Dispersion of Fluorophores for Near-Infrared Fluorescence Imaging In Vivo |
| title_full_unstemmed | Enzyme-Instructed Aggregation/Dispersion of Fluorophores for Near-Infrared Fluorescence Imaging In Vivo |
| title_short | Enzyme-Instructed Aggregation/Dispersion of Fluorophores for Near-Infrared Fluorescence Imaging In Vivo |
| title_sort | enzyme instructed aggregation dispersion of fluorophores for near infrared fluorescence imaging in vivo |
| topic | near-infrared fluorescence enzyme activatable probe |
| url | https://www.mdpi.com/1420-3049/28/14/5360 |
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