| Summary: | Natural diatomite (DA) contains over 63% silica was chemically modified by amine groups of the coupling agent - 3-amino propyl triethoxysilane before deposition of Cu2+ ions. The mixture of Cu2+/DA in chitosan stabilizer at 1% was irradiated by electron beam for reduction of Cu2+ ions to Cu0, and then aggregation into copper nanoparticles (CuNPs) fixing to the interior walls of porous DA forming nanocomposite – CuNPs/DA. Characterizations of copper nanoparticles were determined by UV-Vis spectra, Transmission Electron Micrography images, Scanning Electron Microscopy - Energy Dispersive X-ray spectroscopy, X ray diffraction patterns and Brunauer-Emmett-Teller specific surface area. The antibacterial activities of CuNPs/DA samples corresponding to the content of CuNPs on DA were confirmed by testing paper disk diffusion and MIC against Edwardsiella ictaluri bacteria, a pathogenous agent for Tra catfish (Pangasianodon hypophthalmus).
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