Enhancing bile tolerance improves survival and persistence of <it>Bifidobacterium </it>and <it>Lactococcus </it>in the murine gastrointestinal tract
<p>Abstract</p> <p>Background</p> <p>The majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. However, unlike pathogenic bacteria; they are often inadequately equipped to endure the phys...
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| Format: | Article |
| Language: | English |
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BMC
2008-10-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/8/176 |
| _version_ | 1818347802541948928 |
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| author | Hill Colin Sleator Roy D Watson Debbie Gahan Cormac GM |
| author_facet | Hill Colin Sleator Roy D Watson Debbie Gahan Cormac GM |
| author_sort | Hill Colin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. However, unlike pathogenic bacteria; they are often inadequately equipped to endure the physicochemical stresses of gastrointestinal (GI) delivery in the host. Herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain <it>Bifidobacterium breve </it>UCC2003 and the generally regarded as safe (GRAS) organism <it>Lactococcus lactis </it>NZ9000.</p> <p>Results</p> <p><it>In vitro </it>bile tolerance of both strains was significantly enhanced (<it>P </it>< 0.001), following heterologous expression of the <it>Listeria monocytogenes </it>bile resistance mechanism BilE. Strains harbouring <it>bilE </it>were also recovered at significantly higher levels (<it>P </it>< 0.001), than control strains from the faeces and intestines of mice (<it>n </it>= 5), following oral inoculation. Furthermore, a <it>B. breve </it>strain expressing <it>bilE</it> demonstrated increased efficacy relative to the wild-type strain in reducing oral <it>L. monocytogenes </it>infection in mice.</p> <p>Conclusion</p> <p>Collectively the data indicates that bile tolerance can be enhanced in <it>Bifidobacterium </it>and <it>Lactococcus </it>species through rational genetic manipulation and that this can significantly improve delivery to and colonisation of the GI tract.</p> |
| first_indexed | 2024-12-13T17:39:57Z |
| format | Article |
| id | doaj.art-0d5cfb0b33574e1797285f905f33a956 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-13T17:39:57Z |
| publishDate | 2008-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-0d5cfb0b33574e1797285f905f33a9562022-12-21T23:36:48ZengBMCBMC Microbiology1471-21802008-10-018117610.1186/1471-2180-8-176Enhancing bile tolerance improves survival and persistence of <it>Bifidobacterium </it>and <it>Lactococcus </it>in the murine gastrointestinal tractHill ColinSleator Roy DWatson DebbieGahan Cormac GM<p>Abstract</p> <p>Background</p> <p>The majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. However, unlike pathogenic bacteria; they are often inadequately equipped to endure the physicochemical stresses of gastrointestinal (GI) delivery in the host. Herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain <it>Bifidobacterium breve </it>UCC2003 and the generally regarded as safe (GRAS) organism <it>Lactococcus lactis </it>NZ9000.</p> <p>Results</p> <p><it>In vitro </it>bile tolerance of both strains was significantly enhanced (<it>P </it>< 0.001), following heterologous expression of the <it>Listeria monocytogenes </it>bile resistance mechanism BilE. Strains harbouring <it>bilE </it>were also recovered at significantly higher levels (<it>P </it>< 0.001), than control strains from the faeces and intestines of mice (<it>n </it>= 5), following oral inoculation. Furthermore, a <it>B. breve </it>strain expressing <it>bilE</it> demonstrated increased efficacy relative to the wild-type strain in reducing oral <it>L. monocytogenes </it>infection in mice.</p> <p>Conclusion</p> <p>Collectively the data indicates that bile tolerance can be enhanced in <it>Bifidobacterium </it>and <it>Lactococcus </it>species through rational genetic manipulation and that this can significantly improve delivery to and colonisation of the GI tract.</p>http://www.biomedcentral.com/1471-2180/8/176 |
| spellingShingle | Hill Colin Sleator Roy D Watson Debbie Gahan Cormac GM Enhancing bile tolerance improves survival and persistence of <it>Bifidobacterium </it>and <it>Lactococcus </it>in the murine gastrointestinal tract BMC Microbiology |
| title | Enhancing bile tolerance improves survival and persistence of <it>Bifidobacterium </it>and <it>Lactococcus </it>in the murine gastrointestinal tract |
| title_full | Enhancing bile tolerance improves survival and persistence of <it>Bifidobacterium </it>and <it>Lactococcus </it>in the murine gastrointestinal tract |
| title_fullStr | Enhancing bile tolerance improves survival and persistence of <it>Bifidobacterium </it>and <it>Lactococcus </it>in the murine gastrointestinal tract |
| title_full_unstemmed | Enhancing bile tolerance improves survival and persistence of <it>Bifidobacterium </it>and <it>Lactococcus </it>in the murine gastrointestinal tract |
| title_short | Enhancing bile tolerance improves survival and persistence of <it>Bifidobacterium </it>and <it>Lactococcus </it>in the murine gastrointestinal tract |
| title_sort | enhancing bile tolerance improves survival and persistence of it bifidobacterium it and it lactococcus it in the murine gastrointestinal tract |
| url | http://www.biomedcentral.com/1471-2180/8/176 |
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