A Novel Monocyte Subset as a Unique Signature of Atherosclerotic Plaque Rupture
The evaluation of monocyte subset distribution among acute coronary syndrome (ACS) patients according to culprit coronary plaque morphology has never been explored. We evaluated whether there were significant differences in frequency of circulating monocyte subsets isolated from ACS patients accordi...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.753223/full |
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| author | Ramona Vinci Daniela Pedicino Daniela Pedicino Alice Bonanni Alessia D’Aiello Anna Severino Eugenia Pisano Myriana Ponzo Francesco Canonico Pellegrino Ciampi Giulio Russo Marianna Di Sario Rocco Antonio Montone Carlo Trani Carlo Trani Cristina Conte Maria Chiara Grimaldi Francesco Cribari Massimo Massetti Massimo Massetti Filippo Crea Filippo Crea Giovanna Liuzzo Giovanna Liuzzo |
| author_facet | Ramona Vinci Daniela Pedicino Daniela Pedicino Alice Bonanni Alessia D’Aiello Anna Severino Eugenia Pisano Myriana Ponzo Francesco Canonico Pellegrino Ciampi Giulio Russo Marianna Di Sario Rocco Antonio Montone Carlo Trani Carlo Trani Cristina Conte Maria Chiara Grimaldi Francesco Cribari Massimo Massetti Massimo Massetti Filippo Crea Filippo Crea Giovanna Liuzzo Giovanna Liuzzo |
| author_sort | Ramona Vinci |
| collection | DOAJ |
| description | The evaluation of monocyte subset distribution among acute coronary syndrome (ACS) patients according to culprit coronary plaque morphology has never been explored. We evaluated whether there were significant differences in frequency of circulating monocyte subsets isolated from ACS patients according to optical coherence tomography (OCT) investigation of plaque erosion and rupture. We enrolled 74 patients with non-ST-elevation ACS (NSTE-ACS), 21 of them underwent OCT investigation of the culprit coronary plaque and local macrophage infiltration (MØI) assessment. As control, we enrolled 30 chronic coronary syndrome (CCS) patients. We assessed the frequency of monocyte subsets in the whole study population, in reliance on their CD14 and CD16 expression (classical, CM: CD14++CD16–; intermediates, IM: CD14++CD16+; non-classical, NCM: CD14+CD16++). Then, we tested the effect of lipopolysaccharide (LPS) (a CD14 ligand) on peripheral blood mononuclear cells (PBMCs) of NSTE-ACS patients, quantifying the inflammatory cytokine levels in cell-culture supernatants. Our data proved that monocyte subsets isolated from NSTE-ACS patients represent a peculiar biological signature of the pathophysiological mechanism lying beneath atherosclerotic plaque with a ruptured fibrous cap (RFC) as compared with plaque erosion. Moreover, the magnitude of LPS-mediated effects on IL-1β, IL-6, and IL-10 cytokine release in cell-culture supernatants appeared to be greater in NSTE-ACS patients with RFC. Finally, we described a fourth monocyte population never explored before in this clinical setting (pre-classical monocytes, PCM: CD14+CD16–) that was prevalent in NSTE-ACS patients as compared with CCS and, especially, in patients with RFC and culprit plaque with MØI. |
| first_indexed | 2024-12-19T22:21:25Z |
| format | Article |
| id | doaj.art-0d5f18f464734f14b8091db5e5266e73 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-19T22:21:25Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-0d5f18f464734f14b8091db5e5266e732022-12-21T20:03:37ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-10-01910.3389/fcell.2021.753223753223A Novel Monocyte Subset as a Unique Signature of Atherosclerotic Plaque RuptureRamona Vinci0Daniela Pedicino1Daniela Pedicino2Alice Bonanni3Alessia D’Aiello4Anna Severino5Eugenia Pisano6Myriana Ponzo7Francesco Canonico8Pellegrino Ciampi9Giulio Russo10Marianna Di Sario11Rocco Antonio Montone12Carlo Trani13Carlo Trani14Cristina Conte15Maria Chiara Grimaldi16Francesco Cribari17Massimo Massetti18Massimo Massetti19Filippo Crea20Filippo Crea21Giovanna Liuzzo22Giovanna Liuzzo23Department of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, ItalyDepartment of Cardiovascular and Pneumological Sciences, Catholic University of the Sacred Heart, Rome, ItalyDepartment of Cardiovascular Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, ItalyThe evaluation of monocyte subset distribution among acute coronary syndrome (ACS) patients according to culprit coronary plaque morphology has never been explored. We evaluated whether there were significant differences in frequency of circulating monocyte subsets isolated from ACS patients according to optical coherence tomography (OCT) investigation of plaque erosion and rupture. We enrolled 74 patients with non-ST-elevation ACS (NSTE-ACS), 21 of them underwent OCT investigation of the culprit coronary plaque and local macrophage infiltration (MØI) assessment. As control, we enrolled 30 chronic coronary syndrome (CCS) patients. We assessed the frequency of monocyte subsets in the whole study population, in reliance on their CD14 and CD16 expression (classical, CM: CD14++CD16–; intermediates, IM: CD14++CD16+; non-classical, NCM: CD14+CD16++). Then, we tested the effect of lipopolysaccharide (LPS) (a CD14 ligand) on peripheral blood mononuclear cells (PBMCs) of NSTE-ACS patients, quantifying the inflammatory cytokine levels in cell-culture supernatants. Our data proved that monocyte subsets isolated from NSTE-ACS patients represent a peculiar biological signature of the pathophysiological mechanism lying beneath atherosclerotic plaque with a ruptured fibrous cap (RFC) as compared with plaque erosion. Moreover, the magnitude of LPS-mediated effects on IL-1β, IL-6, and IL-10 cytokine release in cell-culture supernatants appeared to be greater in NSTE-ACS patients with RFC. Finally, we described a fourth monocyte population never explored before in this clinical setting (pre-classical monocytes, PCM: CD14+CD16–) that was prevalent in NSTE-ACS patients as compared with CCS and, especially, in patients with RFC and culprit plaque with MØI.https://www.frontiersin.org/articles/10.3389/fcell.2021.753223/fullmonocyte subsetsacute coronary syndromesplaque ruptureinflammationinnate immunity |
| spellingShingle | Ramona Vinci Daniela Pedicino Daniela Pedicino Alice Bonanni Alessia D’Aiello Anna Severino Eugenia Pisano Myriana Ponzo Francesco Canonico Pellegrino Ciampi Giulio Russo Marianna Di Sario Rocco Antonio Montone Carlo Trani Carlo Trani Cristina Conte Maria Chiara Grimaldi Francesco Cribari Massimo Massetti Massimo Massetti Filippo Crea Filippo Crea Giovanna Liuzzo Giovanna Liuzzo A Novel Monocyte Subset as a Unique Signature of Atherosclerotic Plaque Rupture Frontiers in Cell and Developmental Biology monocyte subsets acute coronary syndromes plaque rupture inflammation innate immunity |
| title | A Novel Monocyte Subset as a Unique Signature of Atherosclerotic Plaque Rupture |
| title_full | A Novel Monocyte Subset as a Unique Signature of Atherosclerotic Plaque Rupture |
| title_fullStr | A Novel Monocyte Subset as a Unique Signature of Atherosclerotic Plaque Rupture |
| title_full_unstemmed | A Novel Monocyte Subset as a Unique Signature of Atherosclerotic Plaque Rupture |
| title_short | A Novel Monocyte Subset as a Unique Signature of Atherosclerotic Plaque Rupture |
| title_sort | novel monocyte subset as a unique signature of atherosclerotic plaque rupture |
| topic | monocyte subsets acute coronary syndromes plaque rupture inflammation innate immunity |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.753223/full |
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