BLACK AND WHITE LAHAR AS INORGANIC SUPPORT FOR THE IMMOBILIZATION OF YEAST INVERTASE
Volcanic ejecta or lahar can serve as an inorganic support for the immobilization of invertase. Pampanga and Bicol lahar samples were pretreated by ignition at 550oC for 5 hrs followed by concentrated hydrochloric acid treatment, activatedby reaction with aminopropyltriethoxysilane (APTS) and then c...
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| Format: | Article |
| Language: | English |
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Universiti Brunei Darussalam
2017-11-01
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| Series: | ASEAN Journal on Science and Technology for Development |
| Online Access: | http://www.ajstd.org/index.php/ajstd/article/view/169 |
| _version_ | 1797332160654344192 |
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| author | Veronica C. Sabularse Mayrene T. Tud Marivic S. Lacsamana Josefina L. Solivas |
| author_facet | Veronica C. Sabularse Mayrene T. Tud Marivic S. Lacsamana Josefina L. Solivas |
| author_sort | Veronica C. Sabularse |
| collection | DOAJ |
| description | Volcanic ejecta or lahar can serve as an inorganic support for the immobilization of invertase. Pampanga and Bicol lahar samples were pretreated by ignition at 550oC for 5 hrs followed by concentrated hydrochloric acid treatment, activatedby reaction with aminopropyltriethoxysilane (APTS) and then covalently bound to invertase using glutaraldehyde as linker. Chemical tests confirmed the attachment of APTS to lahar and glutaraldehyde to silanized lahar. The quantity of immobilized invertase on Pampanga white, Pampanga black and Bicol black lahar were 98.73%, 96.73% and 84.27%,respectively. Conditions for maximum activity of invertase immobilized on Pampanga white lahar were pH 3.5, 45oC and 0.3 M sucrose concentration. The Kmand Vmaxfor free invertase and immobilized invertase on Pampanga white lahar were 2.37 M and 48.75 mmol/min, and 3.88 M and 38.87 mmol/min, respectively. Invertase bound to Pampanga white lahar was most stable towards repeated and continuous use and towards storage with intermittent use as indicated by its relatively greater activity. |
| first_indexed | 2024-03-08T07:45:33Z |
| format | Article |
| id | doaj.art-0d631f5395de43afbc2ed6c045104a89 |
| institution | Directory Open Access Journal |
| issn | 0217-5460 2224-9028 |
| language | English |
| last_indexed | 2024-03-08T07:45:33Z |
| publishDate | 2017-11-01 |
| publisher | Universiti Brunei Darussalam |
| record_format | Article |
| series | ASEAN Journal on Science and Technology for Development |
| spelling | doaj.art-0d631f5395de43afbc2ed6c045104a892024-02-02T16:11:35ZengUniversiti Brunei DarussalamASEAN Journal on Science and Technology for Development0217-54602224-90282017-11-0122433134410.29037/ajstd.169164BLACK AND WHITE LAHAR AS INORGANIC SUPPORT FOR THE IMMOBILIZATION OF YEAST INVERTASEVeronica C. Sabularse0Mayrene T. Tud1Marivic S. Lacsamana2Josefina L. Solivas3Institute of Chemistry, College of Arts and Sciences, UP Los Baños, College, Laguna 4031Institute of Chemistry, College of Arts and Sciences, UP Los Baños, College, Laguna 4031Institute of Chemistry, College of Arts and Sciences, UP Los Baños, College, Laguna 4031Institute of Chemistry, College of Arts and Sciences, UP Los Baños, College, Laguna 4031Volcanic ejecta or lahar can serve as an inorganic support for the immobilization of invertase. Pampanga and Bicol lahar samples were pretreated by ignition at 550oC for 5 hrs followed by concentrated hydrochloric acid treatment, activatedby reaction with aminopropyltriethoxysilane (APTS) and then covalently bound to invertase using glutaraldehyde as linker. Chemical tests confirmed the attachment of APTS to lahar and glutaraldehyde to silanized lahar. The quantity of immobilized invertase on Pampanga white, Pampanga black and Bicol black lahar were 98.73%, 96.73% and 84.27%,respectively. Conditions for maximum activity of invertase immobilized on Pampanga white lahar were pH 3.5, 45oC and 0.3 M sucrose concentration. The Kmand Vmaxfor free invertase and immobilized invertase on Pampanga white lahar were 2.37 M and 48.75 mmol/min, and 3.88 M and 38.87 mmol/min, respectively. Invertase bound to Pampanga white lahar was most stable towards repeated and continuous use and towards storage with intermittent use as indicated by its relatively greater activity.http://www.ajstd.org/index.php/ajstd/article/view/169 |
| spellingShingle | Veronica C. Sabularse Mayrene T. Tud Marivic S. Lacsamana Josefina L. Solivas BLACK AND WHITE LAHAR AS INORGANIC SUPPORT FOR THE IMMOBILIZATION OF YEAST INVERTASE ASEAN Journal on Science and Technology for Development |
| title | BLACK AND WHITE LAHAR AS INORGANIC SUPPORT FOR THE IMMOBILIZATION OF YEAST INVERTASE |
| title_full | BLACK AND WHITE LAHAR AS INORGANIC SUPPORT FOR THE IMMOBILIZATION OF YEAST INVERTASE |
| title_fullStr | BLACK AND WHITE LAHAR AS INORGANIC SUPPORT FOR THE IMMOBILIZATION OF YEAST INVERTASE |
| title_full_unstemmed | BLACK AND WHITE LAHAR AS INORGANIC SUPPORT FOR THE IMMOBILIZATION OF YEAST INVERTASE |
| title_short | BLACK AND WHITE LAHAR AS INORGANIC SUPPORT FOR THE IMMOBILIZATION OF YEAST INVERTASE |
| title_sort | black and white lahar as inorganic support for the immobilization of yeast invertase |
| url | http://www.ajstd.org/index.php/ajstd/article/view/169 |
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