Phytochemical characterization and assessment of antitumor activity of the aqueous extract of Acmella caulirhiza in female Wistar rats induced by 7,12 dimethylbenz (a) anthracene
Background: Recent studies increasingly show the implication of medicinal plants in cancer management. Acmella caulirhiza, a plant belonging to the Asteraceae is traditionally used as an analgesic and anti-inflammatory agent to fight against diseases like cancer. Purpose: This study aimed to evaluat...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031324000058 |
| _version_ | 1797306843985346560 |
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| author | Huiny Miriane Tienoue Fotso Mary-Ann Mbong Angie Françoise Raïssa Ntentie Felix Nwang Ngalla Inelle Makamwe Ferdinand Lanvin Edoun Ebouel Stephane Zingue Julius Oben Enyong |
| author_facet | Huiny Miriane Tienoue Fotso Mary-Ann Mbong Angie Françoise Raïssa Ntentie Felix Nwang Ngalla Inelle Makamwe Ferdinand Lanvin Edoun Ebouel Stephane Zingue Julius Oben Enyong |
| author_sort | Huiny Miriane Tienoue Fotso |
| collection | DOAJ |
| description | Background: Recent studies increasingly show the implication of medicinal plants in cancer management. Acmella caulirhiza, a plant belonging to the Asteraceae is traditionally used as an analgesic and anti-inflammatory agent to fight against diseases like cancer. Purpose: This study aimed to evaluate the antitumoral properties of the aqueous extract of leaves and flowers of A. caulirhiza (AE-Ac). Methods: After phytochemical characterization, the cytotoxic activity of AE-Ac on MCF-7 breast cancer lines was carried out using the MTT method. For in vivo study, 36 female Wistar rats (6 weeks old) were randomly divided into 6 groups (n = 6 each) as follows: normal control receiving distilled water (NC), positive control receiving distilled water (DMBA), 3 test groups (receiving either 75, 150 or 300 mg/kg.Bw of AE-Ac (AE-Ac 75, AE-Ac 150 and AE-Ac 300 respectively)) and a reference group (Tam) receiving Tamoxifen at 3.3 mg/kg.Bw. They were fed with a high-fat diet daily. After 2 weeks all groups except the NC group were treated with a single dose of DMBA (7,12-Dimethylbenz (a) anthracene) (50 mg/kg. Bw) by intraperitoneal injection. Daily treatments were administered by intragastric intubation for 12 weeks. At the end, animals were sacrificed after 12 h of fasting and blood was collected for biochemical analysis. The mammary glands were isolated for histological analysis. Results: AE-Ac contains bioactive compounds amongst which stigmasterol, phytol, ellagic acid, phenol, sterols, alkaloids, flavonoids. AE-Ac was more active at 72 h (IC50 = 114.86 µg/mL) on MCF-7 cells. The cancer-treated groups exhibited the lowest percentage of weight gain variation, particularly the group receiving the reference molecule (Tamoxifen) (68.12 %) followed by the AE-Ac 150 group (92.45 %). AE-Ac at 150 and 300 mg/kg-Bw significantly reduced tumor volume and CA 15-3 levels (p = 0.004). Histological analysis of the mammary gland revealed more extensive ascini destruction in the AE-Ac 75 group followed by the DMBA group. Treatment with the 150 mg/kg.Bw ameliorated antioxidant enzyme (SOD and Catalase (p < 0.001)) activities, pro-oxidant levels (NO● and HO●), as well as inflammatory parameters (TNF-α and EGF; RBC, Hb, PLT) (p < 0.05). Conclusion: These results suggest that AE-Ac can be a good candidate as a complementary agent in breast cancer management. |
| first_indexed | 2024-03-08T00:47:41Z |
| format | Article |
| id | doaj.art-0d69ca2427204c9b948427294789525e |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-03-08T00:47:41Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-0d69ca2427204c9b948427294789525e2024-02-15T05:26:28ZengElsevierPhytomedicine Plus2667-03132024-05-0142100527Phytochemical characterization and assessment of antitumor activity of the aqueous extract of Acmella caulirhiza in female Wistar rats induced by 7,12 dimethylbenz (a) anthraceneHuiny Miriane Tienoue Fotso0Mary-Ann Mbong Angie1Françoise Raïssa Ntentie2Felix Nwang Ngalla3Inelle Makamwe4Ferdinand Lanvin Edoun Ebouel5Stephane Zingue6Julius Oben Enyong7Department of Biochemistry, Faculty of Sciences, The University of Yaounde 1, P.O. Box: 812, Yaounde, CameroonDepartment of Biochemistry, Faculty of Sciences, The University of Yaounde 1, P.O. Box: 812, Yaounde, Cameroon; Corresponding author at: Department of Biochemistry, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon.Department of Biology, Higher Teachers’ Training College, The University of Yaounde 1, P.O. Box: 47, Yaounde, CameroonDepartment of Biochemistry, Faculty of Sciences, The University of Yaounde 1, P.O. Box: 812, Yaounde, CameroonDepartment of Biochemistry, Faculty of Sciences, The University of Yaounde 1, P.O. Box: 812, Yaounde, CameroonDepartment of Biochemistry, Faculty of Sciences, The University of Yaounde 1, P.O. Box: 812, Yaounde, Cameroon; Centre for Food and Nutrition Research, Institute of Medical Research and Medicinal Plant Studies, MINRESI, P.O. Box: 13033, Yaounde, CameroonResearch department of the Faculty of Medicine and Biomedical Sciences, The University of Yaounde 1, P.O. Box: 1364, Yaounde, CameroonDepartment of Biochemistry, Faculty of Sciences, The University of Yaounde 1, P.O. Box: 812, Yaounde, CameroonBackground: Recent studies increasingly show the implication of medicinal plants in cancer management. Acmella caulirhiza, a plant belonging to the Asteraceae is traditionally used as an analgesic and anti-inflammatory agent to fight against diseases like cancer. Purpose: This study aimed to evaluate the antitumoral properties of the aqueous extract of leaves and flowers of A. caulirhiza (AE-Ac). Methods: After phytochemical characterization, the cytotoxic activity of AE-Ac on MCF-7 breast cancer lines was carried out using the MTT method. For in vivo study, 36 female Wistar rats (6 weeks old) were randomly divided into 6 groups (n = 6 each) as follows: normal control receiving distilled water (NC), positive control receiving distilled water (DMBA), 3 test groups (receiving either 75, 150 or 300 mg/kg.Bw of AE-Ac (AE-Ac 75, AE-Ac 150 and AE-Ac 300 respectively)) and a reference group (Tam) receiving Tamoxifen at 3.3 mg/kg.Bw. They were fed with a high-fat diet daily. After 2 weeks all groups except the NC group were treated with a single dose of DMBA (7,12-Dimethylbenz (a) anthracene) (50 mg/kg. Bw) by intraperitoneal injection. Daily treatments were administered by intragastric intubation for 12 weeks. At the end, animals were sacrificed after 12 h of fasting and blood was collected for biochemical analysis. The mammary glands were isolated for histological analysis. Results: AE-Ac contains bioactive compounds amongst which stigmasterol, phytol, ellagic acid, phenol, sterols, alkaloids, flavonoids. AE-Ac was more active at 72 h (IC50 = 114.86 µg/mL) on MCF-7 cells. The cancer-treated groups exhibited the lowest percentage of weight gain variation, particularly the group receiving the reference molecule (Tamoxifen) (68.12 %) followed by the AE-Ac 150 group (92.45 %). AE-Ac at 150 and 300 mg/kg-Bw significantly reduced tumor volume and CA 15-3 levels (p = 0.004). Histological analysis of the mammary gland revealed more extensive ascini destruction in the AE-Ac 75 group followed by the DMBA group. Treatment with the 150 mg/kg.Bw ameliorated antioxidant enzyme (SOD and Catalase (p < 0.001)) activities, pro-oxidant levels (NO● and HO●), as well as inflammatory parameters (TNF-α and EGF; RBC, Hb, PLT) (p < 0.05). Conclusion: These results suggest that AE-Ac can be a good candidate as a complementary agent in breast cancer management.http://www.sciencedirect.com/science/article/pii/S2667031324000058Acmella caulirhizaAntioxidant activityAnti-inflammatory activityLC/GC–MSTumor size |
| spellingShingle | Huiny Miriane Tienoue Fotso Mary-Ann Mbong Angie Françoise Raïssa Ntentie Felix Nwang Ngalla Inelle Makamwe Ferdinand Lanvin Edoun Ebouel Stephane Zingue Julius Oben Enyong Phytochemical characterization and assessment of antitumor activity of the aqueous extract of Acmella caulirhiza in female Wistar rats induced by 7,12 dimethylbenz (a) anthracene Phytomedicine Plus Acmella caulirhiza Antioxidant activity Anti-inflammatory activity LC/GC–MS Tumor size |
| title | Phytochemical characterization and assessment of antitumor activity of the aqueous extract of Acmella caulirhiza in female Wistar rats induced by 7,12 dimethylbenz (a) anthracene |
| title_full | Phytochemical characterization and assessment of antitumor activity of the aqueous extract of Acmella caulirhiza in female Wistar rats induced by 7,12 dimethylbenz (a) anthracene |
| title_fullStr | Phytochemical characterization and assessment of antitumor activity of the aqueous extract of Acmella caulirhiza in female Wistar rats induced by 7,12 dimethylbenz (a) anthracene |
| title_full_unstemmed | Phytochemical characterization and assessment of antitumor activity of the aqueous extract of Acmella caulirhiza in female Wistar rats induced by 7,12 dimethylbenz (a) anthracene |
| title_short | Phytochemical characterization and assessment of antitumor activity of the aqueous extract of Acmella caulirhiza in female Wistar rats induced by 7,12 dimethylbenz (a) anthracene |
| title_sort | phytochemical characterization and assessment of antitumor activity of the aqueous extract of acmella caulirhiza in female wistar rats induced by 7 12 dimethylbenz a anthracene |
| topic | Acmella caulirhiza Antioxidant activity Anti-inflammatory activity LC/GC–MS Tumor size |
| url | http://www.sciencedirect.com/science/article/pii/S2667031324000058 |
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