Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposes
The search for a colorless, nontoxic and efficient drug to prevent transfusion-associated Chagas' disease (TACD) has been underway unsuccessfully since 1953 when gentian violet was preconized and to date is still being used as the only in vitro trypanocidal agent. The recent findings of aminoqu...
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| Format: | Article |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2002-01-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822002000600003 |
| _version_ | 1811232950164914176 |
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| author | Moraes-Souza Helio Pianetti Gisele Marilia Barretto Orlando César de Oliveira Pereira Nonoyama Kimyo Grolg M. Chiari Egler |
| author_facet | Moraes-Souza Helio Pianetti Gisele Marilia Barretto Orlando César de Oliveira Pereira Nonoyama Kimyo Grolg M. Chiari Egler |
| author_sort | Moraes-Souza Helio |
| collection | DOAJ |
| description | The search for a colorless, nontoxic and efficient drug to prevent transfusion-associated Chagas' disease (TACD) has been underway unsuccessfully since 1953 when gentian violet was preconized and to date is still being used as the only in vitro trypanocidal agent. The recent findings of aminoquinolone "WR6026" as a trypanocidal agent, led the authors to study the metabolism of red cells stored with this compound, the main objective of which was to define its applicability in TACD control. Ten units of human whole blood collected in CPDA-1 were divided into two equal satellite bags. One had "WR6026" (final concentration 62.5µg/mL) added and the other was used as a control, both were stored at 4ºC. At baseline, day 7, 14, 21 and 28, samples were taken for the following measurements: adenosine triphosphate (ATP), hemoglobin, electrolytes (sodium and potassium), gases (pO2 and pCO2) and osmotic fragility. The results of tests and control were analyzed through parametric t-student test. The results were similar in both groups throughout the experiment except for the level of ATP on day 14, which presented significantly higher values in the tests when compared with the controls (p = 0.012). It was concluded that WR6026 does not interfere in the preservation and probably the viability of the erythrocytes also until day 28 of storage. Consequently the authors suggest that WR6026 could emerge as a colorless substitute for gentian violet in the control of TACD in endemic areas. |
| first_indexed | 2024-04-12T11:13:05Z |
| format | Article |
| id | doaj.art-0d6a61a31ad241cc9404cc42809288ef |
| institution | Directory Open Access Journal |
| issn | 0037-8682 1678-9849 |
| language | English |
| last_indexed | 2024-04-12T11:13:05Z |
| publishDate | 2002-01-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-0d6a61a31ad241cc9404cc42809288ef2022-12-22T03:35:34ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical0037-86821678-98492002-01-01356563569Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposesMoraes-Souza HelioPianetti Gisele MariliaBarretto Orlando César de Oliveira PereiraNonoyama KimyoGrolg M.Chiari EglerThe search for a colorless, nontoxic and efficient drug to prevent transfusion-associated Chagas' disease (TACD) has been underway unsuccessfully since 1953 when gentian violet was preconized and to date is still being used as the only in vitro trypanocidal agent. The recent findings of aminoquinolone "WR6026" as a trypanocidal agent, led the authors to study the metabolism of red cells stored with this compound, the main objective of which was to define its applicability in TACD control. Ten units of human whole blood collected in CPDA-1 were divided into two equal satellite bags. One had "WR6026" (final concentration 62.5µg/mL) added and the other was used as a control, both were stored at 4ºC. At baseline, day 7, 14, 21 and 28, samples were taken for the following measurements: adenosine triphosphate (ATP), hemoglobin, electrolytes (sodium and potassium), gases (pO2 and pCO2) and osmotic fragility. The results of tests and control were analyzed through parametric t-student test. The results were similar in both groups throughout the experiment except for the level of ATP on day 14, which presented significantly higher values in the tests when compared with the controls (p = 0.012). It was concluded that WR6026 does not interfere in the preservation and probably the viability of the erythrocytes also until day 28 of storage. Consequently the authors suggest that WR6026 could emerge as a colorless substitute for gentian violet in the control of TACD in endemic areas.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822002000600003Transfusion-associated Chagas' diseaseChemoprophylaxisAminoquinoloneBlood transfusion |
| spellingShingle | Moraes-Souza Helio Pianetti Gisele Marilia Barretto Orlando César de Oliveira Pereira Nonoyama Kimyo Grolg M. Chiari Egler Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposes Revista da Sociedade Brasileira de Medicina Tropical Transfusion-associated Chagas' disease Chemoprophylaxis Aminoquinolone Blood transfusion |
| title | Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposes |
| title_full | Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposes |
| title_fullStr | Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposes |
| title_full_unstemmed | Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposes |
| title_short | Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposes |
| title_sort | aminoquinolone wr6026 as a feasible substitute for gentian violet in chagas disease prophylaxis in preserved blood for transfusional purposes |
| topic | Transfusion-associated Chagas' disease Chemoprophylaxis Aminoquinolone Blood transfusion |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822002000600003 |
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