Molecular Basis for Erythromycin Resistance in Group A Streptococcus Isolated From Skin and Soft Tissue Infections
Background: In recent years there has been an increase in the use of erythromycin in the treatment of infections caused by bacteria other than Group A Streptococcus (GAS), which has resulted in increased resistance to this antibiotic. Erythromycin and other macrolides are alternative agents for...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/6843/14744_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
| _version_ | 1819225066349527040 |
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| author | Sunil Shivekar Thangam Menon |
| author_facet | Sunil Shivekar Thangam Menon |
| author_sort | Sunil Shivekar |
| collection | DOAJ |
| description | Background: In recent years there has been an increase in
the use of erythromycin in the treatment of infections caused by
bacteria other than Group A Streptococcus (GAS), which has
resulted in increased resistance to this antibiotic. Erythromycin and
other macrolides are alternative agents for treating GAS infections
in patients, who are allergic to penicillin and its derivatives.
Aim: The main aim of this study was to identify frequency, pattern
and genetic determinant of erythromycin resistance among the
GAS isolated from skin and soft tissue infections.
Materials and Methods: A total 100 isolates of GAS were
screened for erythromycin resistance by phenotypic and genotypic
method.
Results: The results of the present study showed that
38% isolates were resistant to erythromycin. The iMLS
(inducible macrolide-lincosamide-streptogramin) phenotype
was predominant (55.26%) followed by M phenotype (26.32%)
and cMLS (constitutive macrolide-lincosamide-streptogramin)
(18.42%).
Conclusion: Phenotypic and genotypic analysis showed
that the MLSB phenotype with ermB mediated mechanism of
resistance was found the most common (76.31%) followed by
mefA (20.51%). The ermTR genes was absent in all the isolates. |
| first_indexed | 2024-12-23T10:03:41Z |
| format | Article |
| id | doaj.art-0d6d4e7394934a09a8a73737c69a2b79 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-23T10:03:41Z |
| publishDate | 2015-11-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-0d6d4e7394934a09a8a73737c69a2b792022-12-21T17:51:08ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-11-01911DC21DC2310.7860/JCDR/2015/14744.6843Molecular Basis for Erythromycin Resistance in Group A Streptococcus Isolated From Skin and Soft Tissue InfectionsSunil Shivekar0Thangam Menon1PhD Scholar, Department of Microbiology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India.Director and HOD, Department of Microbiology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India.Background: In recent years there has been an increase in the use of erythromycin in the treatment of infections caused by bacteria other than Group A Streptococcus (GAS), which has resulted in increased resistance to this antibiotic. Erythromycin and other macrolides are alternative agents for treating GAS infections in patients, who are allergic to penicillin and its derivatives. Aim: The main aim of this study was to identify frequency, pattern and genetic determinant of erythromycin resistance among the GAS isolated from skin and soft tissue infections. Materials and Methods: A total 100 isolates of GAS were screened for erythromycin resistance by phenotypic and genotypic method. Results: The results of the present study showed that 38% isolates were resistant to erythromycin. The iMLS (inducible macrolide-lincosamide-streptogramin) phenotype was predominant (55.26%) followed by M phenotype (26.32%) and cMLS (constitutive macrolide-lincosamide-streptogramin) (18.42%). Conclusion: Phenotypic and genotypic analysis showed that the MLSB phenotype with ermB mediated mechanism of resistance was found the most common (76.31%) followed by mefA (20.51%). The ermTR genes was absent in all the isolates.https://jcdr.net/articles/PDF/6843/14744_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdferythromycin resistancegenotypesphenotypes |
| spellingShingle | Sunil Shivekar Thangam Menon Molecular Basis for Erythromycin Resistance in Group A Streptococcus Isolated From Skin and Soft Tissue Infections Journal of Clinical and Diagnostic Research erythromycin resistance genotypes phenotypes |
| title | Molecular Basis for Erythromycin Resistance in Group A Streptococcus Isolated From Skin and Soft Tissue Infections |
| title_full | Molecular Basis for Erythromycin Resistance in Group A Streptococcus Isolated From Skin and Soft Tissue Infections |
| title_fullStr | Molecular Basis for Erythromycin Resistance in Group A Streptococcus Isolated From Skin and Soft Tissue Infections |
| title_full_unstemmed | Molecular Basis for Erythromycin Resistance in Group A Streptococcus Isolated From Skin and Soft Tissue Infections |
| title_short | Molecular Basis for Erythromycin Resistance in Group A Streptococcus Isolated From Skin and Soft Tissue Infections |
| title_sort | molecular basis for erythromycin resistance in group a streptococcus isolated from skin and soft tissue infections |
| topic | erythromycin resistance genotypes phenotypes |
| url | https://jcdr.net/articles/PDF/6843/14744_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
| work_keys_str_mv | AT sunilshivekar molecularbasisforerythromycinresistanceingroupastreptococcusisolatedfromskinandsofttissueinfections AT thangammenon molecularbasisforerythromycinresistanceingroupastreptococcusisolatedfromskinandsofttissueinfections |