Two-stage fermentation for lactic acid production on distillery stillage
The aim of this study was to assess utilization of distillery stillage in a two-stage fermentation using Bacillus licheniformis TFUNS and Lactobacillus paracasei NRRL B-4564. In the first stage the stillage was pretreated with B. licheniformis which is an efficient producer of proteolytic enzymes, i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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National Society of Processing and Energy in Agriculture, Novi Sad
2018-01-01
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| Series: | Journal on Processing and Energy in Agriculture |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1821-4487/2018/1821-44871803133M.pdf |
| _version_ | 1811210879573688320 |
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| author | Mladenović Dragana Đukić-Vuković Aleksandra Radosavljević Miloš Pejin Jelena Kocić-Tanackov Sunčica Mojović Ljiljana |
| author_facet | Mladenović Dragana Đukić-Vuković Aleksandra Radosavljević Miloš Pejin Jelena Kocić-Tanackov Sunčica Mojović Ljiljana |
| author_sort | Mladenović Dragana |
| collection | DOAJ |
| description | The aim of this study was to assess utilization of distillery stillage in a two-stage fermentation using Bacillus licheniformis TFUNS and Lactobacillus paracasei NRRL B-4564. In the first stage the stillage was pretreated with B. licheniformis which is an efficient producer of proteolytic enzymes, in order to increase the content of free α-amino nitrogen in the waste substrate. In the subsequent stage the lactic acid (LA) fermentation by L. paracasei was performed. The results of the fermentation of proteolytically pretreated stillage (i.e. of two-stage fermentation) were compared with the untreated stillage (one-stage fermentation). The results have shown that the amount of free α-amino nitrogen in pretreated media was 107 % higher compared to the initial value. The concentration of the LA obtained in the second stage by L. paracasei was 48 % higher than in untreated stillage. In addition, the growth of L. paracasei in pretreated stillage was also better supported compared to that in untreated media. The process enabled economical and sufficient supply of easily assimilative nitrogen sources needed for lactic acid bacteria, thus avoiding addition of costly sources in the media commonly performed in LA production. |
| first_indexed | 2024-04-12T05:03:17Z |
| format | Article |
| id | doaj.art-0d700de2632844c4b377d0516b48167b |
| institution | Directory Open Access Journal |
| issn | 1821-4487 2956-0195 |
| language | English |
| last_indexed | 2024-04-12T05:03:17Z |
| publishDate | 2018-01-01 |
| publisher | National Society of Processing and Energy in Agriculture, Novi Sad |
| record_format | Article |
| series | Journal on Processing and Energy in Agriculture |
| spelling | doaj.art-0d700de2632844c4b377d0516b48167b2022-12-22T03:46:56ZengNational Society of Processing and Energy in Agriculture, Novi SadJournal on Processing and Energy in Agriculture1821-44872956-01952018-01-0122313313710.5937/JPEA1803133M1821-44871803133MTwo-stage fermentation for lactic acid production on distillery stillageMladenović Dragana0https://orcid.org/0000-0003-3218-3692Đukić-Vuković Aleksandra1https://orcid.org/0000-0002-0750-2754Radosavljević Miloš2https://orcid.org/0000-0001-6473-5282Pejin Jelena3Kocić-Tanackov Sunčica4https://orcid.org/0000-0002-9120-6033Mojović Ljiljana5https://orcid.org/0000-0001-7529-4670University of Belgrade, Faculty of Technology and Metallurgy, SerbiaUniversity of Belgrade, Faculty of Technology and Metallurgy, SerbiaUniversity of Novi Sad, Faculty of Technology, SerbiaUniversity of Novi Sad, Faculty of Technology, SerbiaUniversity of Novi Sad, Faculty of Technology, SerbiaUniversity of Belgrade, Faculty of Technology and Metallurgy, SerbiaThe aim of this study was to assess utilization of distillery stillage in a two-stage fermentation using Bacillus licheniformis TFUNS and Lactobacillus paracasei NRRL B-4564. In the first stage the stillage was pretreated with B. licheniformis which is an efficient producer of proteolytic enzymes, in order to increase the content of free α-amino nitrogen in the waste substrate. In the subsequent stage the lactic acid (LA) fermentation by L. paracasei was performed. The results of the fermentation of proteolytically pretreated stillage (i.e. of two-stage fermentation) were compared with the untreated stillage (one-stage fermentation). The results have shown that the amount of free α-amino nitrogen in pretreated media was 107 % higher compared to the initial value. The concentration of the LA obtained in the second stage by L. paracasei was 48 % higher than in untreated stillage. In addition, the growth of L. paracasei in pretreated stillage was also better supported compared to that in untreated media. The process enabled economical and sufficient supply of easily assimilative nitrogen sources needed for lactic acid bacteria, thus avoiding addition of costly sources in the media commonly performed in LA production.https://scindeks-clanci.ceon.rs/data/pdf/1821-4487/2018/1821-44871803133M.pdfdistillery stillagelactic acidtwo-stage fermentationbacillus licheniformislactobacillus paracasei |
| spellingShingle | Mladenović Dragana Đukić-Vuković Aleksandra Radosavljević Miloš Pejin Jelena Kocić-Tanackov Sunčica Mojović Ljiljana Two-stage fermentation for lactic acid production on distillery stillage Journal on Processing and Energy in Agriculture distillery stillage lactic acid two-stage fermentation bacillus licheniformis lactobacillus paracasei |
| title | Two-stage fermentation for lactic acid production on distillery stillage |
| title_full | Two-stage fermentation for lactic acid production on distillery stillage |
| title_fullStr | Two-stage fermentation for lactic acid production on distillery stillage |
| title_full_unstemmed | Two-stage fermentation for lactic acid production on distillery stillage |
| title_short | Two-stage fermentation for lactic acid production on distillery stillage |
| title_sort | two stage fermentation for lactic acid production on distillery stillage |
| topic | distillery stillage lactic acid two-stage fermentation bacillus licheniformis lactobacillus paracasei |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1821-4487/2018/1821-44871803133M.pdf |
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