Evaluation of Genetic Distances of Rice Varieties using RAPD Markers

In rice breeding programs through hybridization, information on genetic distances and similarities between varieties is necessary for development of new lines. This study conducted with the aim of evaluating genetic distances between sixteen rice varieties using Random Amplified Polymorphic DNA (RAP...

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Main Author: Ghaffar Kiyani
Format: Article
Language:fas
Published: Shahid Bahonar University of Kerman 2014-02-01
Series:مجله بیوتکنولوژی کشاورزی
Subjects:
Online Access:https://jab.uk.ac.ir/article_1229_f5a5a0c6a1d497295d7135994d1a5c7c.pdf
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author Ghaffar Kiyani
author_facet Ghaffar Kiyani
author_sort Ghaffar Kiyani
collection DOAJ
description In rice breeding programs through hybridization, information on genetic distances and similarities between varieties is necessary for development of new lines. This study conducted with the aim of evaluating genetic distances between sixteen rice varieties using Random Amplified Polymorphic DNA (RAPD) analysis. Eight out of fifteen primers generated polymorphism and the number of amplification products generated by each primer varied from 4 (OPB-04) to 11 (OPD-11) with an average of 7.75 bands per primer. Out of 62 bands, 43 (69.35 percent) were found to be polymorphic between varieties. Cluster analysis grouped genotypes into 5 clusters. Results of the study showed that varieties Hashemi and Pouya had more genetic distance from each other and from the rest varieties. The information obtained in this study will be helpful in selecting genetically diverse parents for designing rice breeding program in Iran.
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spelling doaj.art-0d711dd60d8f449e9b545cbf9c3b49772022-12-22T01:26:37ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002014-02-015416517310.22103/jab.2014.12291229Evaluation of Genetic Distances of Rice Varieties using RAPD MarkersGhaffar KiyaniIn rice breeding programs through hybridization, information on genetic distances and similarities between varieties is necessary for development of new lines. This study conducted with the aim of evaluating genetic distances between sixteen rice varieties using Random Amplified Polymorphic DNA (RAPD) analysis. Eight out of fifteen primers generated polymorphism and the number of amplification products generated by each primer varied from 4 (OPB-04) to 11 (OPD-11) with an average of 7.75 bands per primer. Out of 62 bands, 43 (69.35 percent) were found to be polymorphic between varieties. Cluster analysis grouped genotypes into 5 clusters. Results of the study showed that varieties Hashemi and Pouya had more genetic distance from each other and from the rest varieties. The information obtained in this study will be helpful in selecting genetically diverse parents for designing rice breeding program in Iran.https://jab.uk.ac.ir/article_1229_f5a5a0c6a1d497295d7135994d1a5c7c.pdf: ricegenetic distancesrapd marker
spellingShingle Ghaffar Kiyani
Evaluation of Genetic Distances of Rice Varieties using RAPD Markers
مجله بیوتکنولوژی کشاورزی
: rice
genetic distances
rapd marker
title Evaluation of Genetic Distances of Rice Varieties using RAPD Markers
title_full Evaluation of Genetic Distances of Rice Varieties using RAPD Markers
title_fullStr Evaluation of Genetic Distances of Rice Varieties using RAPD Markers
title_full_unstemmed Evaluation of Genetic Distances of Rice Varieties using RAPD Markers
title_short Evaluation of Genetic Distances of Rice Varieties using RAPD Markers
title_sort evaluation of genetic distances of rice varieties using rapd markers
topic : rice
genetic distances
rapd marker
url https://jab.uk.ac.ir/article_1229_f5a5a0c6a1d497295d7135994d1a5c7c.pdf
work_keys_str_mv AT ghaffarkiyani evaluationofgeneticdistancesofricevarietiesusingrapdmarkers