CHANGES IN THE MITOCHONDRIAL APPARATUS OF CARDIOMYOCYTES UNDER THE INFLUENCE OF OPIOID IN THE EXPERIMENT
This scientific work presents theoretical conclusions and a new solution to the scientific task, which consists in determining the features of the structure and some biochemical parameters of the blood and organs of the cardiac muscle of a white rat under normal conditions and under long-term opioid...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Ukrainian Medical Stomatological Academy
2022-11-01
|
| Series: | Вісник проблем біології і медицини |
| Subjects: | |
| Online Access: | https://vpbm.com.ua/upload/2022-3 (166)/61-min.pdf |
| _version_ | 1811221448307507200 |
|---|---|
| author | Pokotylo P. B. Fedevych Yu. M. Denysenko N. V. Logash M. V. Genyk I. D. |
| author_facet | Pokotylo P. B. Fedevych Yu. M. Denysenko N. V. Logash M. V. Genyk I. D. |
| author_sort | Pokotylo P. B. |
| collection | DOAJ |
| description | This scientific work presents theoretical conclusions and a new solution to the scientific task, which consists in determining the features of the structure and some biochemical parameters of the blood and organs of the cardiac muscle of a white rat under normal conditions and under long-term opioid exposure. The experiment was conducted on 53 white male rats with a body weight of 130-210 g. Animals were divided into 3 groups – experimental (30 animals), control (18 animals) and 5 animals to check the standards before the experiment. The use of optical and electron microscopy confirmed that the heart muscle of the white rat is composed of muscle fibers that branch and intertwine to form a specific mesh. Myofibrils, on the other hand, consist of a chain of sarcomeres separated by Z-lines, each containing 2-3 mitochondria of all 3 types. There are 3 types of mitochondria in the sarcoplasm of cardiomyocytes: 1 type of elongated mitochondria, which have a relatively large volume and a well-developed complex of cristae; mitochondria of the 2nd type have a rounded shape, which, compared to the mitochondria of the I type, have a smaller volume and a smaller number of cristae; 3 type of mitochondria of very small volume, but with a large number of crystals. Cardiomyocytes are connected to each other with the help of intercalated disks. The gaps between cardiomyocytes are filled with loose connective tissue with nerves and components of the microcirculatory bed represented by arterioles, precapillary arterioles, capillaries of the somatic, unfenestrated type, postcapillary venules and venules. The first signs of damage to the microstructure of the myocardium are visible 7 days after the administration of nalbuphine to white rats. During the next 35 days of the experiment, the pathological changes manifested by the violation of the well-defined structure of the heart muscle, fragmentation of muscle fibers, pronounced perivascular edema and fibrosis increase. Small-caliber vessels are markedly hyperemic with “sludge syndrome” in the lumen of microvessels. Administration of nalbuphine causes well-defined changes in the ultrastructure of the rat heart. First of all, it is manifested in a change in the shape, size of mitochondria, structure and number of cristae. In addition, there were changes in the nuclei of parenchymal cells – they acquired a non-uniform shape due to chromatin fragmentation. This may indicate the beginning of apoptosis. In addition, there are changes in the structure of microvascular blood flow – changes in the endothelial coating of microvessels of the heart, occlusion of coronary vessels, aggregation of erythrocytes and platelets on the luminal surface of endotheliocytes. |
| first_indexed | 2024-04-12T08:00:35Z |
| format | Article |
| id | doaj.art-0d732b371d8b4e968a880d50173a09e4 |
| institution | Directory Open Access Journal |
| issn | 2077-4214 2523-4110 |
| language | English |
| last_indexed | 2024-04-12T08:00:35Z |
| publishDate | 2022-11-01 |
| publisher | Ukrainian Medical Stomatological Academy |
| record_format | Article |
| series | Вісник проблем біології і медицини |
| spelling | doaj.art-0d732b371d8b4e968a880d50173a09e42022-12-22T03:41:20ZengUkrainian Medical Stomatological AcademyВісник проблем біології і медицини2077-42142523-41102022-11-01143644110.29254/2077-4214-2022-3-166-436-4412077-4214-2022-3-166-436-441CHANGES IN THE MITOCHONDRIAL APPARATUS OF CARDIOMYOCYTES UNDER THE INFLUENCE OF OPIOID IN THE EXPERIMENTPokotylo P. B.0Fedevych Yu. M.1Denysenko N. V.2Logash M. V.3Genyk I. D.4 Danylo Halytsky Lviv National Medical University Danylo Halytsky Lviv National Medical University Danylo Halytsky Lviv National Medical University Danylo Halytsky Lviv National Medical University Danylo Halytsky Lviv National Medical University This scientific work presents theoretical conclusions and a new solution to the scientific task, which consists in determining the features of the structure and some biochemical parameters of the blood and organs of the cardiac muscle of a white rat under normal conditions and under long-term opioid exposure. The experiment was conducted on 53 white male rats with a body weight of 130-210 g. Animals were divided into 3 groups – experimental (30 animals), control (18 animals) and 5 animals to check the standards before the experiment. The use of optical and electron microscopy confirmed that the heart muscle of the white rat is composed of muscle fibers that branch and intertwine to form a specific mesh. Myofibrils, on the other hand, consist of a chain of sarcomeres separated by Z-lines, each containing 2-3 mitochondria of all 3 types. There are 3 types of mitochondria in the sarcoplasm of cardiomyocytes: 1 type of elongated mitochondria, which have a relatively large volume and a well-developed complex of cristae; mitochondria of the 2nd type have a rounded shape, which, compared to the mitochondria of the I type, have a smaller volume and a smaller number of cristae; 3 type of mitochondria of very small volume, but with a large number of crystals. Cardiomyocytes are connected to each other with the help of intercalated disks. The gaps between cardiomyocytes are filled with loose connective tissue with nerves and components of the microcirculatory bed represented by arterioles, precapillary arterioles, capillaries of the somatic, unfenestrated type, postcapillary venules and venules. The first signs of damage to the microstructure of the myocardium are visible 7 days after the administration of nalbuphine to white rats. During the next 35 days of the experiment, the pathological changes manifested by the violation of the well-defined structure of the heart muscle, fragmentation of muscle fibers, pronounced perivascular edema and fibrosis increase. Small-caliber vessels are markedly hyperemic with “sludge syndrome” in the lumen of microvessels. Administration of nalbuphine causes well-defined changes in the ultrastructure of the rat heart. First of all, it is manifested in a change in the shape, size of mitochondria, structure and number of cristae. In addition, there were changes in the nuclei of parenchymal cells – they acquired a non-uniform shape due to chromatin fragmentation. This may indicate the beginning of apoptosis. In addition, there are changes in the structure of microvascular blood flow – changes in the endothelial coating of microvessels of the heart, occlusion of coronary vessels, aggregation of erythrocytes and platelets on the luminal surface of endotheliocytes. https://vpbm.com.ua/upload/2022-3 (166)/61-min.pdf myocardium,microvasculature,white rat,mitochondria,nalbuphine. |
| spellingShingle | Pokotylo P. B. Fedevych Yu. M. Denysenko N. V. Logash M. V. Genyk I. D. CHANGES IN THE MITOCHONDRIAL APPARATUS OF CARDIOMYOCYTES UNDER THE INFLUENCE OF OPIOID IN THE EXPERIMENT Вісник проблем біології і медицини myocardium,microvasculature,white rat,mitochondria,nalbuphine. |
| title | CHANGES IN THE MITOCHONDRIAL APPARATUS OF CARDIOMYOCYTES UNDER THE INFLUENCE OF OPIOID IN THE EXPERIMENT |
| title_full | CHANGES IN THE MITOCHONDRIAL APPARATUS OF CARDIOMYOCYTES UNDER THE INFLUENCE OF OPIOID IN THE EXPERIMENT |
| title_fullStr | CHANGES IN THE MITOCHONDRIAL APPARATUS OF CARDIOMYOCYTES UNDER THE INFLUENCE OF OPIOID IN THE EXPERIMENT |
| title_full_unstemmed | CHANGES IN THE MITOCHONDRIAL APPARATUS OF CARDIOMYOCYTES UNDER THE INFLUENCE OF OPIOID IN THE EXPERIMENT |
| title_short | CHANGES IN THE MITOCHONDRIAL APPARATUS OF CARDIOMYOCYTES UNDER THE INFLUENCE OF OPIOID IN THE EXPERIMENT |
| title_sort | changes in the mitochondrial apparatus of cardiomyocytes under the influence of opioid in the experiment |
| topic | myocardium,microvasculature,white rat,mitochondria,nalbuphine. |
| url | https://vpbm.com.ua/upload/2022-3 (166)/61-min.pdf |
| work_keys_str_mv | AT pokotylopb changesinthemitochondrialapparatusofcardiomyocytesundertheinfluenceofopioidintheexperiment AT fedevychyum changesinthemitochondrialapparatusofcardiomyocytesundertheinfluenceofopioidintheexperiment AT denysenkonv changesinthemitochondrialapparatusofcardiomyocytesundertheinfluenceofopioidintheexperiment AT logashmv changesinthemitochondrialapparatusofcardiomyocytesundertheinfluenceofopioidintheexperiment AT genykid changesinthemitochondrialapparatusofcardiomyocytesundertheinfluenceofopioidintheexperiment |