DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks
Abstract Two stochastic microdisks were designed and validated for the DNA mismatch repair assessment in gastric and colon cancers, through determining simultaneously the concentrations of MLH1, MSH2, MSH6, PMS2, and of KRAS in whole blood, urine, saliva, and tumoral tissues. The active surface of t...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-11-01
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| Series: | ChemElectroChem |
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| Online Access: | https://doi.org/10.1002/celc.202300273 |
| _version_ | 1797635948567068672 |
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| author | Prof. Dr. Raluca‐Ioana Stefan‐van Staden Alexandru Adrian Bratei Dr. Ruxandra‐Maria Ilie‐Mihai Damaris‐Cristina Gheorghe Bianca Maria Tuchiu |
| author_facet | Prof. Dr. Raluca‐Ioana Stefan‐van Staden Alexandru Adrian Bratei Dr. Ruxandra‐Maria Ilie‐Mihai Damaris‐Cristina Gheorghe Bianca Maria Tuchiu |
| author_sort | Prof. Dr. Raluca‐Ioana Stefan‐van Staden |
| collection | DOAJ |
| description | Abstract Two stochastic microdisks were designed and validated for the DNA mismatch repair assessment in gastric and colon cancers, through determining simultaneously the concentrations of MLH1, MSH2, MSH6, PMS2, and of KRAS in whole blood, urine, saliva, and tumoral tissues. The active surface of the stochastic microdisks was composed of a thin layer of graphene paste decorated with nitrogen, boron, and sulfur and modified with inutec (SP) and with frutafit (FT), respectively. High sensitivities were recorded when the SP was used as modifier. All limits of determination were of fg mL−1 magnitude order. The paired student‐t‐test done at 99.00 % confidence level revealed that there are no significative differences between the results obtained using the two stochastic microdisks. Validation of the screening tests of whole blood, urine, saliva, and tumoral tissues was also done using the recovery tests when % recovery was higher than 87.00 %, with %RSD values lower than 1.00 %. |
| first_indexed | 2024-03-11T12:28:17Z |
| format | Article |
| id | doaj.art-0d73c4f75e29452bb1faa2d254cab69f |
| institution | Directory Open Access Journal |
| issn | 2196-0216 |
| language | English |
| last_indexed | 2024-03-11T12:28:17Z |
| publishDate | 2023-11-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemElectroChem |
| spelling | doaj.art-0d73c4f75e29452bb1faa2d254cab69f2023-11-06T04:46:43ZengWiley-VCHChemElectroChem2196-02162023-11-011021n/an/a10.1002/celc.202300273DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic MicrodisksProf. Dr. Raluca‐Ioana Stefan‐van Staden0Alexandru Adrian Bratei1Dr. Ruxandra‐Maria Ilie‐Mihai2Damaris‐Cristina Gheorghe3Bianca Maria Tuchiu4Laboratory of Electrochemistry and PATLAB National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 RomaniaLaboratory of Electrochemistry and PATLAB National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 RomaniaLaboratory of Electrochemistry and PATLAB National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 RomaniaLaboratory of Electrochemistry and PATLAB National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 RomaniaLaboratory of Electrochemistry and PATLAB National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 RomaniaAbstract Two stochastic microdisks were designed and validated for the DNA mismatch repair assessment in gastric and colon cancers, through determining simultaneously the concentrations of MLH1, MSH2, MSH6, PMS2, and of KRAS in whole blood, urine, saliva, and tumoral tissues. The active surface of the stochastic microdisks was composed of a thin layer of graphene paste decorated with nitrogen, boron, and sulfur and modified with inutec (SP) and with frutafit (FT), respectively. High sensitivities were recorded when the SP was used as modifier. All limits of determination were of fg mL−1 magnitude order. The paired student‐t‐test done at 99.00 % confidence level revealed that there are no significative differences between the results obtained using the two stochastic microdisks. Validation of the screening tests of whole blood, urine, saliva, and tumoral tissues was also done using the recovery tests when % recovery was higher than 87.00 %, with %RSD values lower than 1.00 %.https://doi.org/10.1002/celc.202300273DNA mismatch repairGastric cancerColon cancerMismatch repair proteinsStochastic microdisks |
| spellingShingle | Prof. Dr. Raluca‐Ioana Stefan‐van Staden Alexandru Adrian Bratei Dr. Ruxandra‐Maria Ilie‐Mihai Damaris‐Cristina Gheorghe Bianca Maria Tuchiu DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks ChemElectroChem DNA mismatch repair Gastric cancer Colon cancer Mismatch repair proteins Stochastic microdisks |
| title | DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks |
| title_full | DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks |
| title_fullStr | DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks |
| title_full_unstemmed | DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks |
| title_short | DNA Mismatch Repair Assessment in Gastric and Colon Cancers Using Stochastic Microdisks |
| title_sort | dna mismatch repair assessment in gastric and colon cancers using stochastic microdisks |
| topic | DNA mismatch repair Gastric cancer Colon cancer Mismatch repair proteins Stochastic microdisks |
| url | https://doi.org/10.1002/celc.202300273 |
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